The Journal of Neurophysiology Vol. 86 No. 1 July 2001, pp. 321-325
Copyright ©2001 by the American Physiological Society
Chemical Induction of mGluR5- and Protein Synthesis-Dependent
Long-Term Depression in Hippocampal Area CA1
Kimberly M.
Huber,1
John
C.
Roder,2 and
Mark F.
Bear1
1Department of Neuroscience, Howard Hughes
Medical Institute, Brown University, Providence, Rhode Island 02912;
and 2Program in Developmental and Fetal Health,
Samuel Lunenfeld Research Institute, Toronto, Ontario M5G 1X5, Canada
Huber, Kimberly M.,
John
C. Roder, and
Mark F. Bear.
Chemical Induction of mGluR5- and Protein Synthesis-Dependent
Long-Term Depression in Hippocampal Area CA1. J. Neurophysiol. 86: 321-325, 2001. Recent work has
demonstrated that specific patterns of synaptic stimulation can induce
long-term depression (LTD) in area CA1 that depends on activation of
metabotropic glutamate receptors (mGluRs) and rapid protein synthesis.
Here we show that the same form of synaptic modification can be induced
by brief application of the selective mGluR agonist
(RS)-3,5-dihydroxyphenylglycine (DHPG). DHPG-LTD 1) is a
saturable form of synaptic plasticity, 2) requires mGluR5,
3) is mechanistically distinct from
N-methyl-D-aspartate receptor (NMDAR)-dependent
LTD, and 4) shares a common expression mechanism with
protein synthesis-dependent LTD evoked using synaptic stimulation.
DHPG-LTD should be useful for biochemical analysis of mGluR5- and
protein synthesis-dependent synaptic modification.
