JN Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 87: 361-384, 2002;
0022-3077/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (40)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cruikshank, S. J.
Right arrow Articles by Metherate, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cruikshank, S. J.
Right arrow Articles by Metherate, R.

The Journal of Neurophysiology Vol. 87 No. 1 January 2002, pp. 361-384
Copyright ©2002 by the American Physiological Society

Auditory Thalamocortical Synaptic Transmission In Vitro

Scott J. Cruikshank, Heather J. Rose, and Raju Metherate

Department of Neurobiology and Behavior, University of California, Irvine, California 92697

Cruikshank, Scott J., Heather J. Rose, and Raju Metherate. Auditory Thalamocortical Synaptic Transmission In Vitro. J. Neurophysiol. 87: 361-384, 2002. To facilitate an understanding of auditory thalamocortical mechanisms, we have developed a mouse brain-slice preparation with a functional connection between the ventral division of the medial geniculate (MGv) and the primary auditory cortex (ACx). Here we present the basic characteristics of the slice in terms of physiology (intracellular and extracellular recordings, including current source density analysis), pharmacology (including glutamate receptor involvement), and anatomy (gross anatomy, Nissl, parvalbumin immunocytochemistry, and tract tracing with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate). Thalamocortical transmission in this preparation (the "primary" slice) involves both alpha -amino-3-hydroxy-5-methylisoxazole-4-proprionic acid/kainate and N-methyl-D-aspartate-type glutamate receptors that appear to mediate monosynaptic inputs to layers 3-4 of ACx. MGv stimulation also initiates disynaptic inhibitory postsynaptic potentials and longer-duration intracortical, polysynaptic activity. Important differences between responses elicited by MGv versus conventional columnar ("on-beam") stimulation emphasize the necessity of thalamic activation to infer thalamocortical mechanisms. We also introduce a second slice preparation, the "shell" slice, obtained from the brain region immediately ventral to the primary slice, that may contain a nonprimary thalamocortical pathway to temporal cortex. In the shell slice, stimulation of the thalamus or the region immediately ventral to it appears to produce fast activation of synapses in cortical layer 1 followed by robust intracortical polysynaptic activity. The layer 1 responses may result from orthodromic activation of nonprimary thalamocortical pathways; however, a plausible alternative could involve antidromic activation of corticotectal neurons and their layer 1 collaterals. The primary and shell slices will provide useful tools to investigate mechanisms of information processing in the ACx.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
A.-M. M. Oswald and A. D. Reyes
Maturation of Intrinsic and Synaptic Properties of Layer 2/3 Pyramidal Neurons in Mouse Auditory Cortex
J Neurophysiol, June 1, 2008; 99(6): 2998 - 3008.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
M. Steinschneider, Y. I. Fishman, and J. C. Arezzo
Spectrotemporal Analysis of Evoked and Induced Electroencephalographic Responses in Primary Auditory Cortex (A1) of the Awake Monkey
Cereb Cortex, March 1, 2008; 18(3): 610 - 625.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Xu, V. C. Kotak, and D. H. Sanes
Conductive Hearing Loss Disrupts Synaptic and Spike Adaptation in Developing Auditory Cortex
J. Neurosci., August 29, 2007; 27(35): 9417 - 9426.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
T. Chomiak and B. Hu
Axonal and somatic filtering of antidromically evoked cortical excitation by simulated deep brain stimulation in rat brain
J. Physiol., March 1, 2007; 579(2): 403 - 412.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. C. Kotak, A. D. Breithaupt, and D. H. Sanes
Developmental hearing loss eliminates long-term potentiation in the auditory cortex
PNAS, February 27, 2007; 104(9): 3550 - 3555.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
M.-R. Jafari, Y. Zhang, and J. Yan
Multiparametric Changes in the Receptive Field of Cortical Auditory Neurons Induced by Thalamic Activation in the Mouse
Cereb Cortex, January 1, 2007; 17(1): 71 - 80.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. Soto, N. Kopell, and K. Sen
Network Architecture, Receptive Fields, and Neuromodulation: Computational and Functional Implications of Cholinergic Modulation in Primary Auditory Cortex
J Neurophysiol, December 1, 2006; 96(6): 2972 - 2983.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y. I. Verbny, F. Erdelyi, G. Szabo, and M. I. Banks
Properties of a Population of GABAergic Cells in Murine Auditory Cortex Weakly Excited by Thalamic Stimulation
J Neurophysiol, December 1, 2006; 96(6): 3194 - 3208.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Sasaki, K. Huda, T. Inoue, M. Miyata, and K. Imoto
Impaired feedforward inhibition of the thalamocortical projection in epileptic Ca2+ channel mutant mice, tottering.
J. Neurosci., March 15, 2006; 26(11): 3056 - 3065.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
R. Narayan, A. Ergun, and K. Sen
Delayed Inhibition in Cortical Receptive Fields and the Discrimination of Complex Stimuli
J Neurophysiol, October 1, 2005; 94(4): 2970 - 2975.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H. J. Rose and R. Metherate
Auditory Thalamocortical Transmission Is Reliable and Temporally Precise
J Neurophysiol, September 1, 2005; 94(3): 2019 - 2030.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. C. Kotak, S. Fujisawa, F. A. Lee, O. Karthikeyan, C. Aoki, and D. H. Sanes
Hearing Loss Raises Excitability in the Auditory Cortex
J. Neurosci., April 13, 2005; 25(15): 3908 - 3918.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
M. Steinschneider, I. O. Volkov, Y. I. Fishman, H. Oya, J. C. Arezzo, and M. A. Howard III
Intracortical Responses in Human and Monkey Primary Auditory Cortex Support a Temporal Processing Mechanism for Encoding of the Voice Onset Time Phonetic Parameter
Cereb Cortex, February 1, 2005; 15(2): 170 - 186.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Y. Y. Tan, L. I. Zhang, M. M. Merzenich, and C. E. Schreiner
Tone-Evoked Excitatory and Inhibitory Synaptic Conductances of Primary Auditory Cortex Neurons
J Neurophysiol, July 1, 2004; 92(1): 630 - 643.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Kaur, R. Lazar, and R. Metherate
Intracortical Pathways Determine Breadth of Subthreshold Frequency Receptive Fields in Primary Auditory Cortex
J Neurophysiol, June 1, 2004; 91(6): 2551 - 2567.
[Abstract] [Full Text] [PDF]

Home page
 Learn. Mem.Home page
R. Metherate
Nicotinic Acetylcholine Receptors in Sensory Cortex
Learn. Mem., January 1, 2004; 11(1): 50 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
J. F. Linden and C. E. Schreiner
Columnar Transformations in Auditory Cortex? A Comparison to Visual and Somatosensory Cortices
Cereb Cortex, January 1, 2003; 13(1): 83 - 89.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Beierlein and B. W. Connors
Short-Term Dynamics of Thalamocortical and Intracortical Synapses Onto Layer 6 Neurons in Neocortex
J Neurophysiol, October 1, 2002; 88(4): 1924 - 1932.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online