| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Neurophysiology Vol. 87 No. 2 February 2002, pp. 1132-1137
Copyright ©2002 by the American Physiological Society
RAPID COMMUNICATION
Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030
Liang, Yong,
Li-Lian Yuan,
Daniel Johnston, and
Richard Gray.
Calcium Signaling at Single Mossy Fiber Presynaptic Terminals in
the Rat Hippocampus. J. Neurophysiol. 87: 1132-1137, 2002. We investigated internal
Ca2+ release at mossy fiber synapses on CA3
pyramidal neurons (mossy fiber terminals, MFTs) in the hippocampus.
Presynaptic Ca2+ influx was induced by giving a
brief train of 20 stimuli at 100 Hz to the mossy fiber pathway. Using
Ca2+ imaging techniques, we recorded the
Ca2+ response as 
F/F,
which increased rapidly with stimulation, but was often accompanied by
a delayed peak that occurred after the train. The rise in presynaptic
[Ca2+] could be completely blocked by
application of 400 µM Cd2+. Furthermore, the
evoked Ca2+ signals were reduced by group II
mGluR agonists. Under the same experimental conditions, we investigated
the effects of several agents on MFTs that disrupt regulation of
intracellular Ca2+ stores resulting in depletion
of internal Ca2+. We found that ryanodine,
cyclopiazonic acid, thapsigargin, and ruthenium red all decreased both
the early and the delayed increase in the Ca2+
signals. We applied D,L-2-amino-5-phosphonovaleric acid
(D,L-APV; 50 µM) and 6,7-Dinitroquinoxaline-2,3-dione
(DNQX; 20 µM) to exclude the action of
N-methyl-D-aspartate (NMDA) and non-NMDA
receptors. Experiments with alternative lower affinity indicators for
Ca2+ (fura-2FF and calcium green-2) and the
transient K+ channel blocker, 4-aminopyridine
were performed to control for the possible saturation of fura-2. Taken
together, these results strongly support the hypothesis that the
recorded terminals were from the mossy fibers of the dentate gyrus and
suggest that a portion of the presynaptic Ca2+
signal in response to brief trains of stimuli is due to release of
Ca2+ from internal stores.
This article has been cited by other articles:
|
|
 |
|
  H. Y. Sun, A. F. Bartley, and L. E. Dobrunz Calcium-Permeable Presynaptic Kainate Receptors Involved in Excitatory Short-Term Facilitation Onto Somatostatin Interneurons During Natural Stimulus Patterns J Neurophysiol, February 1, 2009; 101(2): 1043 - 1055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Scott, T. Lalic, D. M. Kullmann, M. Capogna, and D. A. Rusakov Target-Cell Specificity of Kainate Autoreceptor and Ca2+-Store-Dependent Short-Term Plasticity at Hippocampal Mossy Fiber Synapses J. Neurosci., December 3, 2008; 28(49): 13139 - 13149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shimizu, M. Fukaya, M. Yamasaki, M. Watanabe, T. Manabe, and H. Kamiya Use-dependent amplification of presynaptic Ca2+ signaling by axonal ryanodine receptors at the hippocampal mossy fiber synapse PNAS, August 19, 2008; 105(33): 11998 - 12003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Nichols, A. F. Dengler, E. M. Nakagawa, M. Bashkin, B. T. Paul, J. Wu, and G. M. Khan A Constitutive, Transient Receptor Potential-like Ca2+ Influx Pathway in Presynaptic Nerve Endings Independent of Voltage-gated Ca2+ Channels and Na+/Ca2+ Exchange J. Biol. Chem., December 7, 2007; 282(49): 36102 - 36111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Scott and D. A. Rusakov Main determinants of presynaptic Ca2+ dynamics at individual mossy fiber-CA3 pyramidal cell synapses. J. Neurosci., June 28, 2006; 26(26): 7071 - 7081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Moldavan, R. P. Irwin, and C. N. Allen Presynaptic GABAB Receptors Regulate Retinohypothalamic Tract Synaptic Transmission by Inhibiting Voltage-Gated Ca2+ Channels J Neurophysiol, June 1, 2006; 95(6): 3727 - 3741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zissimopoulos, D. J. West, A. J. Williams, and F. A. Lai Ryanodine receptor interaction with the SNARE-associated protein snapin J. Cell Sci., June 1, 2006; 119(11): 2386 - 2397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verkhratsky Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons Physiol Rev, January 1, 2005; 85(1): 201 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. K. Unni, S. S. Zakharenko, L. Zablow, A. J. DeCostanzo, and S. A. Siegelbaum Calcium Release from Presynaptic Ryanodine-Sensitive Stores Is Required for Long-Term Depression at Hippocampal CA3-CA3 Pyramidal Neuron Synapses J. Neurosci., October 27, 2004; 24(43): 9612 - 9622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Martin and W. Buno Caffeine-Mediated Presynaptic Long-Term Potentiation in Hippocampal CA1 Pyramidal Neurons J Neurophysiol, June 1, 2003; 89(6): 3029 - 3038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Jackson and S. J. Redman Calcium Dynamics, Buffering, and Buffer Saturation in the Boutons of Dentate Granule-Cell Axons in the Hilus J. Neurosci., March 1, 2003; 23(5): 1612 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kirischuk and R. Grantyn Inter-Bouton Variability of Synaptic Strength Correlates With Heterogeneity of Presynaptic Ca2+ Signals J Neurophysiol, October 1, 2002; 88(4): 2172 - 2176. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
