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J Neurophysiol 87: 1132-1137, 2002;
0022-3077/02 $5.00
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The Journal of Neurophysiology Vol. 87 No. 2 February 2002, pp. 1132-1137
Copyright ©2002 by the American Physiological Society

RAPID COMMUNICATION

Calcium Signaling at Single Mossy Fiber Presynaptic Terminals in the Rat Hippocampus

Yong Liang, Li-Lian Yuan, Daniel Johnston, and Richard Gray

Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030

Liang, Yong, Li-Lian Yuan, Daniel Johnston, and Richard Gray. Calcium Signaling at Single Mossy Fiber Presynaptic Terminals in the Rat Hippocampus. J. Neurophysiol. 87: 1132-1137, 2002. We investigated internal Ca2+ release at mossy fiber synapses on CA3 pyramidal neurons (mossy fiber terminals, MFTs) in the hippocampus. Presynaptic Ca2+ influx was induced by giving a brief train of 20 stimuli at 100 Hz to the mossy fiber pathway. Using Ca2+ imaging techniques, we recorded the Ca2+ response as Delta F/F, which increased rapidly with stimulation, but was often accompanied by a delayed peak that occurred after the train. The rise in presynaptic [Ca2+] could be completely blocked by application of 400 µM Cd2+. Furthermore, the evoked Ca2+ signals were reduced by group II mGluR agonists. Under the same experimental conditions, we investigated the effects of several agents on MFTs that disrupt regulation of intracellular Ca2+ stores resulting in depletion of internal Ca2+. We found that ryanodine, cyclopiazonic acid, thapsigargin, and ruthenium red all decreased both the early and the delayed increase in the Ca2+ signals. We applied D,L-2-amino-5-phosphonovaleric acid (D,L-APV; 50 µM) and 6,7-Dinitroquinoxaline-2,3-dione (DNQX; 20 µM) to exclude the action of N-methyl-D-aspartate (NMDA) and non-NMDA receptors. Experiments with alternative lower affinity indicators for Ca2+ (fura-2FF and calcium green-2) and the transient K+ channel blocker, 4-aminopyridine were performed to control for the possible saturation of fura-2. Taken together, these results strongly support the hypothesis that the recorded terminals were from the mossy fibers of the dentate gyrus and suggest that a portion of the presynaptic Ca2+ signal in response to brief trains of stimuli is due to release of Ca2+ from internal stores.




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