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The Journal of Neurophysiology Vol. 87 No. 6 June 2002, pp. 3048-3058
Copyright ©2002 by the American Physiological Society
Department of Neurobiology, Institute of Life Sciences and the Interdisciplinary Center for Neuronal Computation, Hebrew University, Jerusalem 91904, Israel
Devor, Anna and
Yosef Yarom.
Electrotonic Coupling in the Inferior Olivary Nucleus Revealed by
Simultaneous Double Patch Recordings. J. Neurophysiol. 87: 3048-3058, 2002. Electrotonic coupling in
the inferior olivary (IO) nucleus is assumed to play a crucial role in
generating the subthreshold membrane potential oscillations in olivary
neurons and in synchronizing climbing fiber input into the cerebellar
cortex. We studied the strength and spatial distribution of the
coupling by simultaneous double patch recordings from olivary neurons
in the brain slice preparation. Electrotonic coupling was observed in
50% of the cell pairs. The coupling coefficient (CC),
defined as the ratio between voltage responses of the post- and the
prejunctional cell, varied between 0.002 and 0.17; most of the pairs
were weakly coupled. In more than 75% of the pairs, the CC
was <0.05. The coupling resistance varied between 0.7 to 19.8 G
,
and 68% of the values fell between 0.7 to 8 G. The difference
between the coupling coefficient measured on stimulation of cell 1 or
cell 2 of a coupled pair was 27 ± 16%. Direct calculation of the
coupling resistance revealed an asymmetry of 24 ± 12%,
suggesting a directional preference of coupling. The coupling was
voltage independent, although depolarization of either the pre- or the
postjunctional neuron reduced the CC. The chance of a cell
pair being coupled was 80% in immediate neighboring cells, but dropped
to about 30% at a distance of 40 µm. No coupled pairs were observed
at distances larger than 70 µm. In 52% of staining experiments
neurobiotin injection into an olivary neuron produced indirect labeling
of 1-11 nearby cells with an average of 3.8 ± 2.9. All
indirectly labeled cells were found in, or immediately adjacent, to the
dendritic field of the directly stained neuron. Two distinct
morphological types of olivary neurons, "curly" and "straight"
cells, were found. In each case all neurons stained indirectly by dye
passage through gap junctions belonged to the same type. Using the
physiological data we estimated that each olivary neuron is directly
coupled to about 50 neurons. Since somatic recordings may not reveal
coupling through remote dendrites, we conclude that each neuron is
directly connected to 
50 neurons forming two distinct networks of
curly and straight cells.
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