JN Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 89: 806-813, 2003. First published October 30, 2002; doi:10.1152/jn.00614.2002
0022-3077/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
89/2/806    most recent
00614.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ziskind-Conhaim, L.
Right arrow Articles by Hinckley, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ziskind-Conhaim, L.
Right arrow Articles by Hinckley, C.

J Neurophysiol (February 1, 2003). 10.1152/jn.00614.2002
Submitted on Submitted 1 August 2002; accepted in final form 15 October 2002

Ethanol Dual Modulatory Actions on Spontaneous Postsynaptic Currents in Spinal Motoneurons

Lea Ziskind-Conhaim, Bao-Xi Gao, and Christopher Hinckley

Department of Physiology and Center for Neuroscience University of Wisconsin Medical School, Madison, Wisconsin 53706

Ziskind-Conhaim, Lea, Bao-Xi Gao, and Christopher Hinckley. Ethanol Dual Modulatory Actions on Spontaneous Postsynaptic Currents in Spinal Motoneurons. J. Neurophysiol. 89: 806-813, 2003. Recently we have shown that acute ethanol (EtOH) exposure suppresses dorsal root-evoked synaptic potentials in spinal motoneurons. To examine the synaptic mechanisms underlying the reduced excitatory activity, EtOH actions on properties of action potential-independent miniature excitatory and inhibitory postsynaptic currents (mEPSCs and mIPSCs) were studied in spinal motoneurons of newborn rats. Properties of mEPSCs generated by activation of N-methyl-D-aspartate receptors (NMDARs) and non-NMDA receptors and of mIPSCs mediated by glycine and gamma -aminobutyric acid-A receptors (GlyR and GABAAR) were examined during acute exposure to 70 and 200 mM EtOH. In the presence of 70 mM EtOH, the frequency of NMDAR- and non-NMDAR-mediated mEPSCs decreased to 53 ± 5 and 45 ± 7% (means ± SE) of control values, respectively. In contrast, the frequency of GlyR- and GABAAR-mediated mIPSCs increased to 138 ± 15 and 167 ± 23% of control, respectively. Based on the quantal theory of transmitter release, changes in the frequency of miniature currents are correlated with changes in transmitter release, suggesting that EtOH decreased presynaptic glutamate release and increased the release of both glycine and GABA. EtOH did not change the amplitude or rise and decay times of either mEPSCs or mIPSCs, indicating that the presynaptic changes were not associated with changes in the properties of postsynaptic receptors/channels. Acute exposure to 200 mM EtOH increased mIPSC frequency two- to threefold, significantly higher than the increase induced by 70 mM EtOH. However, the decrease in mEPSC frequency was similar to that observed in 70 mM EtOH. Those findings implied that the regulatory effect of EtOH on glycine and GABA release was dose-dependent. Exposure to the higher EtOH concentration had opposite actions on mEPSC and mIPSC amplitudes: it attenuated the amplitude of NMDAR- and non-NMDAR-mediated mEPSCs to ~80% of control and increased GlyR- and GABAAR-mediated mIPSC amplitude by ~20%. EtOH-induced changes in the amplitude of postsynaptic currents were not associated with changes in their basic kinetic properties. Our data suggested that in spinal networks of newborn rats, EtOH was more effective in modulating the release of excitatory and inhibitory neurotransmitters than changing the properties of their receptors/channels.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
M. K. Kelm, H. E. Criswell, and G. R. Breese
The Role of Protein Kinase A in the Ethanol-Induced Increase in Spontaneous GABA Release Onto Cerebellar Purkinje Neurons
J Neurophysiol, December 1, 2008; 100(6): 3417 - 3428.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Ziskind-Conhaim, L. Wu, and E. P. Wiesner
Persistent Sodium Current Contributes to Induced Voltage Oscillations in Locomotor-Related Hb9 Interneurons in the Mouse Spinal Cord
J Neurophysiol, October 1, 2008; 100(4): 2254 - 2264.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
H. E. Criswell, Z. Ming, M. K. Kelm, and G. R. Breese
Brain Regional Differences in the Effect of Ethanol on GABA Release from Presynaptic Terminals
J. Pharmacol. Exp. Ther., August 1, 2008; 326(2): 596 - 603.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. A. Hinckley and L. Ziskind-Conhaim
Electrical Coupling between Locomotor-Related Excitatory Interneurons in the Mammalian Spinal Cord.
J. Neurosci., August 15, 2006; 26(33): 8477 - 8483.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. Ziskind-Conhaim and S. Redman
Spatiotemporal Patterns of Dorsal Root-Evoked Network Activity in the Neonatal Rat Spinal Cord: Optical and Intracellular Recordings
J Neurophysiol, September 1, 2005; 94(3): 1952 - 1961.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Mameli, P. A. Zamudio, M. Carta, and C. F. Valenzuela
Developmentally Regulated Actions of Alcohol on Hippocampal Glutamatergic Transmission
J. Neurosci., August 31, 2005; 25(35): 8027 - 8036.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. A. Hinckley, R. Hartley, L. Wu, A. Todd, and L. Ziskind-Conhaim
Locomotor-Like Rhythms in a Genetically Distinct Cluster of Interneurons in the Mammalian Spinal Cord
J Neurophysiol, March 1, 2005; 93(3): 1439 - 1449.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C.-Y. Chen and A. C. Bonham
Glutamate suppresses GABA release via presynaptic metabotropic glutamate receptors at baroreceptor neurones in rats
J. Physiol., January 15, 2005; 562(2): 535 - 551.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. W. Hendricson, J. R. Sibbald, and R. A. Morrisett
Ethanol Alters the Frequency, Amplitude, and Decay Kinetics of Sr2+-Supported, Asynchronous NMDAR mEPSCs in Rat Hippocampal Slices
J Neurophysiol, June 1, 2004; 91(6): 2568 - 2577.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Carta, M. Mameli, and C. F. Valenzuela
Alcohol Enhances GABAergic Transmission to Cerebellar Granule Cells via an Increase in Golgi Cell Excitability
J. Neurosci., April 14, 2004; 24(15): 3746 - 3751.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Y. Sebe, E. D. Eggers, and A. J. Berger
Differential Effects of Ethanol on GABAA and Glycine Receptor-Mediated Synaptic Currents in Brain Stem Motoneurons
J Neurophysiol, August 1, 2003; 90(2): 870 - 875.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online