HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (23) Citing Articles via Google Scholar Google Scholar Articles by Martín, E. D. Articles by Buño, W. Search for Related Content PubMed PubMed Citation Articles by Martín, E. D. Articles by Buño, W.

Caffeine-Mediated Presynaptic Long-Term Potentiation in Hippocampal CA1 Pyramidal Neurons

Instituto Cajal, CSIC, 28002-Madrid, Spain

Submitted 25 July 2002; accepted in final form 6 February 2003

We report a new form of long-term potentiation (LTP) in Schaffer collateral (SC)-CA1 pyramidal neuron synapses that originates presynaptically and does not require N-methyl-D-aspartate (NMDA) receptor activation nor increases in postsynaptic-free Ca²⁺. Using rat hippocampal slices, application of a brief "pulse" of caffeine in the bath evoked a nondecremental LTP (_CAFLTP) of SC excitatory postsynaptic currents. An increased probability of transmitter release paralleled the _CAFLTP, suggesting that it originated presynaptically. The P₁ adenosine receptor antagonist 8-cyclopentyltheophylline and the P₂ purinoreceptor antagonists suramin and piridoxal-5'-phosphate-azophenyl 2',4'-disulphonate blocked the _CAFLTP. Inhibition of Ca²⁺ release from caffeine/ryanodine stores by bath-applied ryanodine inhibited the _CAFLTP, but ryanodine in the pipette solution was ineffective, suggesting a presynaptic effect of ryanodine. Previous induction of the "classical" LTP did not prevent the _CAFLTP, suggesting that the LTP and the _CAFLTP have different underlying cellular mechanisms. The _CAFLTP is insensitive to the block of NMDA receptors by 2-amino-5-phosphonopentanoic acid and to Ca²⁺ chelation with intracellular 1,2-bis (2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid, indicating that neither postsynaptic NMDA receptors nor increases in cytosolic-free Ca²⁺ participate in the _CAFLTP. We conclude that the _CAFLTP requires the interaction of caffeine with presynaptic P₁, P₂ purinoreceptors, and ryanodine receptors and is caused by an increased probability of glutamate release at SC terminals.

This article has been cited by other articles:

				N. Granado, O. Ortiz, L. M. Suarez, E. D. Martin, V. Cena, J. M. Solis, and R. Moratalla D1 but not D5 Dopamine Receptors Are Critical for LTP, Spatial Learning, and LTP-Induced arc and zif268 Expression in the Hippocampus Cereb Cortex, January 1, 2008; 18(1): 1 - 12. [Abstract] [Full Text] [PDF]

				T. Kenet, R. C. Froemke, C. E. Schreiner, I. N. Pessah, and M. M. Merzenich Perinatal exposure to a noncoplanar polychlorinated biphenyl alters tonotopy, receptive fields, and plasticity in rat primary auditory cortex PNAS, May 1, 2007; 104(18): 7646 - 7651. [Abstract] [Full Text] [PDF]

				C. Cabezas and W. Buno Distinct Transmitter Release Properties Determine Differences in Short-Term Plasticity at Functional and Silent Synapses J Neurophysiol, May 1, 2006; 95(5): 3024 - 3034. [Abstract] [Full Text] [PDF]

				D. Fernandez de Sevilla, M. Rodrigo-Angulo, A. Nunez, and W. Buno Cholinergic Modulation of Synaptic Transmission and Postsynaptic Excitability in the Rat Gracilis Dorsal Column Nucleus J. Neurosci., April 12, 2006; 26(15): 4015 - 4025. [Abstract] [Full Text] [PDF]

				A. Verkhratsky Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons Physiol Rev, January 1, 2005; 85(1): 201 - 279. [Abstract] [Full Text] [PDF]