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Department of Biology, Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454-9110
Submitted 27 January 2003; accepted in final form 28 March 2003
To better understand regulation of N-methyl-D-aspartate
(NMDA) and 
-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)
receptor complements across the cortex, and to investigate NMDA receptor
(NMDAR)-based models of persistent activity, we compared NMDA/AMPA ratios in
prefrontal (PFC) and visual cortex (VC) in rat. Whole cell voltage-clamp
responses were recorded in brain slices from layer 2/3 pyramidal cells of the
medial PFC and VC of rats aged p16–p21. Mixed miniature excitatory
postsynaptic currents (mEPSCs) having AMPA receptor (AMPAR)- and
NMDAR-mediated components were isolated in nominally 0
Mg2+ ACSF. Averaged mEPSCs were well-fit by double
exponentials. No significant differences in the NMDA/AMPA ratio (PFC: 27
± 1%; VC: 28 ± 3%), peak mEPSC amplitude (PFC: 19.1 ± 1
pA; VC: 17.5 ± 0.7 pA), NMDAR decay kinetics (PFC: 69 ± 8 ms;
VC: 67 ± 6 ms), or degree of correlation between NMDAR- and
AMPAR-mediated mEPSC components were found between the areas (PFC: n
= 27; VC: n = 28). Recordings from older rats (p26–29) also
showed no differences. EPSCs were evoked extracellularly in 2 mM
Mg2+ at depolarized potentials; although the average
NMDA/AMPA ratio was larger than that observed for mEPSCs, the ratio was
similar in the two regions. In nominally 0 Mg2+ and in
the presence of CNQX, spontaneous activation of NMDAR increased recording
noise and produced a small tonic depolarization which was similar in both
areas. We conclude that this basic property of excitatory transmission is
conserved across PFC and VC synapses and is therefore unlikely to contribute
to differences in firing patterns observed in vivo in the two regions.
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