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Biophysical Properties and Ionic Signature of Neuronal Progenitors of the Postnatal Subventricular Zone In Situ

¹ Department of Neurosurgery and Cellular and Molecular Physiology and, Yale University School of Medicine, New Haven, Connecticut 06520-8082; ² Interdepartmental Neuroscience Graduate Program, Yale University School of Medicine, New Haven, Connecticut 06520-8082

Submitted 12 December 2002; accepted in final form 10 June 2003

Previous studies have reported the presence of neuronal progenitors in the subventricular zone (SVZ) and rostral migratory stream (RMS) of the postnatal mammalian brain. Although many studies have examined the survival and migration of progenitors after transplantation and the factors influencing their proliferation or differentiation, no information is available on the electrophysiological properties of these progenitors in a near-intact environment. Thus we performed whole cell and cell-attached patch-clamp recordings of progenitors in brain slices containing either the SVZ or the RMS from postnatal day 15 to day 25 mice. Both regions displayed strong immunoreactivity for nestin and neuron-specific class III -tubulin, and recorded cells displayed a morphology typical of the neuronal progenitors known to migrate throughout the SVZ and RMS to the olfactory bulb. Recorded progenitors had depolarized zero-current resting potentials (mean more depolarized than –28 mV), very high input resistances (about 4 G), and lacked action potentials. Using the reversal potential of K⁺ currents through a cell-attached patch a mean resting potential of –59 mV was estimated. Recorded progenitors displayed Ca²⁺-dependent K⁺ currents and TEA-sensitive-delayed rectifying K⁺ (K_DR) currents, but lacked inward K⁺ currents and transient outward K⁺ currents. K_DR currents displayed classical kinetics and were also sensitive to 4-aminopyridine and -dendrotoxin, a blocker of Kv1 channels. Na⁺ currents were found in about 60% of the SVZ neuronal progenitors. No developmental changes were observed in the passive membrane properties and current profile of neuronal progenitors. Together these data suggest that SVZ neuronal progenitors display passive membrane properties and an ionic signature distinct from that of cultured SVZ neuronal progenitors and mature neurons.

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