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J Neurophysiol 91: 1006-1012, 2004. First published October 15, 2003; doi:10.1152/jn.00771.2003
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Differences in Glycinergic mIPSCs in the Auditory Brain Stem of Normal and Congenitally Deaf Neonatal Mice

Richardson N. Leao1, Sharon Oleskevich1, Hong Sun2, Melissa Bautista2, Robert E.W. Fyffe2 and Bruce Walmsley1

1Synaptic Structure and Function Group, Division of Neuroscience, The John Curtin School of Medical Research, The Australian National University, Canberra ACT 0200, Australia; and 2Center for Brain Research, Wright State University, Dayton, Ohio 45435

Submitted 7 August 2003; accepted in final form 2 October 2003

We have investigated the fundamental properties of central auditory glycinergic synapses in early postnatal development in normal and congenitally deaf (dn/dn) mice. Glycinergic miniature inhibitory postsynaptic currents (mIPSCs) were recorded using patch-clamp methods in neurons from a brain slice preparation of the medial nucleus of the trapezoid body (MNTB), at 12-14 days postnatal age. Our results show a number of significant differences between normal and deaf mice. The frequency of mIPSCs is greater (50%) in deaf versus normal mice. Mean mIPSC amplitude is smaller in deaf mice than in normal mice (mean mIPSC amplitude: deaf, 64 pA; normal, 106 pA). Peak-scaled fluctuation analysis of mIPSCs showed that mean single channel conductance is greater in the deaf mice (deaf, 64 pS; normal, 45 pS). The mean decay time course of mIPSCs is slower in MNTB neurons from deaf mice (mean half-width: deaf, 2.9 ms; normal, 2.3 ms). Light- and electron-microscopic immunolabeling results showed that MNTB neurons from deaf mice have more (30%) inhibitory synaptic sites (postsynaptic gephyrin clusters) than MNTB neurons in normal mice. Our results demonstrate substantial differences in glycinergic transmission in normal and congenitally deaf mice, supporting a role for activity during development in regulating both synaptic structure (connectivity) and the fundamental (quantal) properties of mIPSCs at central glycinergic synapses.

Address for reprint requests and other correspondence: B. Walmsley, The Synaptic Structure and Function Group, Division of Neuroscience, The John Curtin School of Medical Research, The Australian National University, PO Box 334, Canberra ACT 2601, Australia (E-mail: Bruce.Walmsley{at}anu.edu.au).




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