| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Department of Neurophysiology, Faculty of Medicine, Ruhr-University Bochum, D-44780 Bochum, Germany; 2Institute of Higher Nervous Activity and Neurophysiology RAS, 117865 Moscow, Russia; 3Department of Electrical Engineering and Computer Science, Technical University of Berlin, 10587 Berlin, Germany
Submitted 5 December 2003; accepted in final form 28 February 2004
The probability of transmitter release at synaptic terminals is one of the key characteristics of communication between nerve cells because it determines both the strength and dynamic properties of synaptic connections. To assess the distribution of the release probabilities at excitatory synapses on supragranular pyramidal cells in rat visual cortex, we have used the MK-801, a blocker of the open N-methyl-D-aspartate (NMDA) receptor-gated channels. With this method, the release probability can be calculated from the time course of the blockade of NMDA-receptor mediated postsynaptic currents in the presence of MK-801. At temperatures >32°C, the distribution of release probabilities covered the range from 0.05 to 0.43 [mean: 0.171 ± 0.012 (SE), n = 65], being skewed toward low values. When estimated at room temperature (22–25°C), the release probabilities were significantly lower (mean: 0.123 ± 0.009, n = 54), and almost the whole distribution was restricted to values <0.2. Furthermore, warming from room temperature to >32°C led to a pronounced overshooting increase of the release probability. Taken together, the results of the present study show that release probabilities at synapses formed onto layer 2/3 pyramidal cells in the visual cortex vary significantly, but values >0.3 are rare and the results obtained either at room or variable temperature differ significantly from those made under conditions of constant temperature in the physiological range.
This article has been cited by other articles:
|
|
 |
|
  A.-M. M. Oswald and A. D. Reyes Maturation of Intrinsic and Synaptic Properties of Layer 2/3 Pyramidal Neurons in Mouse Auditory Cortex J Neurophysiol, June 1, 2008; 99(6): 2998 - 3008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhang and M. B. Jackson Temperature Dependence of Fusion Kinetics and Fusion Pores in Ca2+-triggered Exocytosis from PC12 Cells J. Gen. Physiol., January 28, 2008; 131(2): 117 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nouvian Temperature enhances exocytosis efficiency at the mouse inner hair cell ribbon synapse J. Physiol., October 15, 2007; 584(2): 535 - 542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Corlew, Y. Wang, H. Ghermazien, A. Erisir, and B. D. Philpot Developmental Switch in the Contribution of Presynaptic and Postsynaptic NMDA Receptors to Long-Term Depression J. Neurosci., September 12, 2007; 27(37): 9835 - 9845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kushmerick, R. Renden, and H. von Gersdorff Physiological Temperatures Reduce the Rate of Vesicle Pool Depletion and Short-Term Depression via an Acceleration of Vesicle Recruitment J. Neurosci., February 1, 2006; 26(5): 1366 - 1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Rose and R. Metherate Auditory Thalamocortical Transmission Is Reliable and Temporally Precise J Neurophysiol, September 1, 2005; 94(3): 2019 - 2030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Micheva and S. J. Smith Strong Effects of Subphysiological Temperature on the Function and Plasticity of Mammalian Presynaptic Terminals J. Neurosci., August 17, 2005; 25(33): 7481 - 7488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-F. Yang, Y. Ouyang, B. R Kennedy, and S. M Rothman Cooling blocks rat hippocampal neurotransmission by a presynaptic mechanism: observations using 2-photon microscopy J. Physiol., August 15, 2005; 567(1): 215 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Beck, M. Chistiakova, K. Obermayer, and M. Volgushev Adaptation at Synaptic Connections to Layer 2/3 Pyramidal Cells in Rat Visual Cortex J Neurophysiol, July 1, 2005; 94(1): 363 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. H. van den Burg, R. R. Peeters, M. Verhoye, J. Meek, G. Flik, and A. Van der Linden Brain Responses to Ambient Temperature Fluctuations in Fish: Reduction of Blood Volume and Initiation of a Whole-Body Stress Response J Neurophysiol, May 1, 2005; 93(5): 2849 - 2855. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
