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J Neurophysiol 92: 2909-2919, 2004. First published July 7, 2004; doi:10.1152/jn.01198.2003
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Taste Receptor Cells Express pH-Sensitive Leak K+ Channels

W. Lin1,2, C. A. Burks4, D. R. Hansen4, S. C. Kinnamon2,3 and T. A. Gilbertson4

1Cell and Developmental Biology and 2The Rocky Mountain Taste and Smell Center, University of Colorado Health Sciences Center at Fitzsimons, Aurora 80045; 3Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523; and 4Department of Biology and The Center for Integrated BioSystems, Utah State University, Logan, Utah 84322

Submitted 11 December 2003; accepted in final form 6 July 2004

Two-pore domain K+ channels encoded by genes KCNK1-17 (K2p1-17) play important roles in regulating cell excitability. We report here that rat taste receptor cells (TRCs) highly express TASK-2 (KCNK5; K2p5.1), and to a much lesser extent TALK-1 (KCNK16; K2p16.1) and TASK-1 (KCNK3; K2p3.1), and suggest potentially important roles for these channels in setting resting membrane potentials and in sour taste transduction. Whole cell recordings of isolated TRCs show that a leak K+ (Kleak) current in a subset of TRCs exhibited high sensitivity to acidic extracellular pH similar to reported properties of TASK-2 and TALK-1 channels. A drop in bath pH from 7.4 to 6 suppressed 90% of the current, resulting in membrane depolarization. K+ channel blockers, BaCl2, but not tetraethylammonium (TEA), inhibited the current. Interestingly, resting potentials of these TRCs averaged –70 mV, which closely correlated with the amplitude of the pH-sensitive Kleak, suggesting a dominant role of this conductance in setting resting potentials. RT-PCR assays followed by sequencing of PCR products showed that TASK-1, TASK-2, and a functionally similar channel, TALK-1, were expressed in all three types of lingual taste buds. To verify expression of TASK channels, we labeled taste tissue with antibodies against TASK-1, TASK-2, and TASK-3. Strong labeling was seen in some TRCs with antibody against TASK-2 but not TASK-1 and TASK-3. Consistent with the immunocytochemical staining, quantitative real-time PCR assays showed that the message for TASK-2 was expressed at significantly higher levels (10–100 times greater) than was TASK-1, TALK-1, or TASK-3. Thus several K2P channels, and in particular TASK-2, are expressed in rat TRCs, where they may contribute to the establishment of resting potentials and sour reception.

Address for reprint requests and other correspondence: T. A. Gilbertson, Dept. of Biology and Center for Integrated BioSystems, Utah State Univ., Logan, UT 84322-5305 (E-mail: tag{at}biology.usu.edu).




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