JN Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 93: 94-107, 2005. First published June 30, 2004; doi:10.1152/jn.00270.2004
0022-3077/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
93/1/94    most recent
00270.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wong, K. Y.
Right arrow Articles by Dowling, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, K. Y.
Right arrow Articles by Dowling, J. E.

Retinal Bipolar Cell Input Mechanisms in Giant Danio. II. Patch-Clamp Analysis of ON Bipolar Cells

Kwoon Y. Wong, Ethan D. Cohen and John E. Dowling

Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts

Submitted 18 March 2004; accepted in final form 23 June 2004

Glutamate receptors on giant danio retinal ON bipolar cells were studied with whole cell patch clamping using a slice preparation. Cone-driven ON bipolars (Cbs) and mixed-input ON bipolars (Mbs) were identified morphologically. Most Cbs responded to the excitatory amino acid transporter (EAAT) substrate D-aspartate but not to the group III metabotropic glutamate receptor (mGluR) agonist L-(+)-2-amino-4-phosphonobutyric acid (L-AP4) or the AMPA/kainate receptor agonist kainate, suggesting EAATs are the primary glutamate receptors on Cbs. The EAAT inhibitor DL-threo-{beta}-benzyloxyasparate (TBOA) blocked all light-evoked responses of Cbs, suggesting these responses are mediated exclusively by EAATs. Conversely, all Mbs responded to D-aspartate and L-AP4 but not to kainate, indicating they have both EAATs and group III mGluRs (presumably mGluR6). The light responses of Mbs involve both receptors because they could be blocked by TBOA plus (RS)-{alpha}-cyclopropyl-4-phosphonophenylglycine (CPPG, a group III mGluR antagonist) but not by either alone. Under dark-adapted conditions, the responses of Mbs to green (rod-selective) stimuli were reduced by CPPG but enhanced by TBOA. In contrast, both antagonists reduced the responses to red (cone-selective) stimuli, although TBOA was more effective. Furthermore, under photopic conditions, TBOA failed to eliminate light-evoked responses of Mbs. Thus on Mbs, rod inputs are mediated predominantly by mGluR6, whereas cone inputs are mediated mainly by EAATs but also by mGluR6 to some extent. Finally, we explored the interactions between EAATs and mGluR6 in Mbs. Responses to D-aspartate were reduced by L-AP4 and vice versa. Therefore mGluR6 and EAATs suppress each other, and this might underlie mutual suppression between rod and cone signals in Mbs.

Address for reprint requests and other correspondence: K. Y. Wong, Department of Neuroscience, Box 1953, Brown University, Providence, RI 02912 (E-mail: Kwoon_Wong{at}brown.edu)




This article has been cited by other articles:


Home page
 J. Physiol.Home page
Y. Ping, H. Huang, X.-J. Zhang, and X.-L. Yang
Melatonin potentiates rod signals to ON type bipolar cells in fish retina
J. Physiol., June 1, 2008; 586(11): 2683 - 2694.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Emran, J. Rihel, A. R. Adolph, K. Y. Wong, S. Kraves, and J. E. Dowling
OFF ganglion cells cannot drive the optokinetic reflex in zebrafish
PNAS, November 27, 2007; 104(48): 19126 - 19131.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. Y. Wong, F. A. Dunn, D. M. Graham, and D. M. Berson
Synaptic influences on rat ganglion-cell photoreceptors
J. Physiol., July 1, 2007; 582(1): 279 - 296.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D.-Q. Zhang, T.-R. Zhou, and D. G. McMahon
Functional Heterogeneity of Retinal Dopaminergic Neurons Underlying Their Multiple Roles in Vision
J. Neurosci., January 17, 2007; 27(3): 692 - 699.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
E. Wersinger, Y. Schwab, J.-A. Sahel, A. Rendon, D. V. Pow, S. Picaud, and M. J. Roux
The glutamate transporter EAAT5 works as a presynaptic receptor in mouse rod bipolar cells
J. Physiol., November 15, 2006; 577(1): 221 - 234.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. I. Matsui, A. L. Egana, T. R. Sponholtz, A. R. Adolph, and J. E. Dowling
Effects of ethanol on photoreceptors and visual function in developing zebrafish.
Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4589 - 4597.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Vigh and H. von Gersdorff
Prolonged Reciprocal Signaling via NMDA and GABA Receptors at a Retinal Ribbon Synapse
J. Neurosci., December 7, 2005; 25(49): 11412 - 11423.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Y. Wong and J. E. Dowling
Retinal Bipolar Cell Input Mechanisms in Giant Danio. III. ON-OFF Bipolar Cells and Their Color-Opponent Mechanisms
J Neurophysiol, July 1, 2005; 94(1): 265 - 272.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. P. Dryja, T. L. McGee, E. L. Berson, G. A. Fishman, M. A. Sandberg, K. R. Alexander, D. J. Derlacki, and A. S. Rajagopalan
Night blindness and abnormal cone electroretinogram ON responses in patients with mutations in the GRM6 gene encoding mGluR6
PNAS, March 29, 2005; 102(13): 4884 - 4889.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the The American Physiological Society.