HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 93/5/2987    most recent 01023.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Blanche, T. J. Articles by Swindale, N. V. Search for Related Content PubMed PubMed Citation Articles by Blanche, T. J. Articles by Swindale, N. V.

INNOVATIVE METHODOLOGY

Polytrodes: High-Density Silicon Electrode Arrays for Large-Scale Multiunit Recording

¹Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, British Columbia, Canada; and ²Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan

Submitted 28 September 2004; accepted in final form 14 November 2004

We developed a variety of 54-channel high-density silicon electrode arrays (polytrodes) designed to record from large numbers of neurons spanning millimeters of brain. In cat visual cortex, it was possible to make simultaneous recordings from >100 well-isolated neurons. Using standard clustering methods, polytrodes provide a quality of single-unit isolation that surpasses that attainable with tetrodes. Guidelines for successful in vivo recording and precise electrode positioning are described. We also describe a high-bandwidth continuous data-acquisition system designed specifically for polytrodes and an automated impedance meter for testing polytrode site integrity. Despite having smaller interconnect pitches than earlier silicon-based electrodes of this type, these polytrodes have negligible channel crosstalk, comparable reliability, and low site impedances and are capable of making high-fidelity multiunit recordings with minimal tissue damage. The relatively benign nature of planar electrode arrays is evident both histologically and in experiments where the polytrode was repeatedly advanced and retracted hundreds of microns over periods of many hours. It was possible to maintain stable recordings from active neurons adjacent to the polytrode without change in their absolute positions, neurophysiological or receptive field properties.

This article has been cited by other articles:

				J. Du, I. H. Riedel-Kruse, J. C. Nawroth, M. L. Roukes, G. Laurent, and S. C. Masmanidis High-Resolution Three-Dimensional Extracellular Recording of Neuronal Activity With Microfabricated Electrode Arrays J Neurophysiol, March 1, 2009; 101(3): 1671 - 1678. [Abstract] [Full Text] [PDF]

				K. A. Ludwig, R. M. Miriani, N. B. Langhals, M. D. Joseph, D. J. Anderson, and D. R. Kipke Using a Common Average Reference to Improve Cortical Neuron Recordings From Microelectrode Arrays J Neurophysiol, March 1, 2009; 101(3): 1679 - 1689. [Abstract] [Full Text] [PDF]

				H. Merchant, T. Naselaris, and A. P. Georgopoulos Dynamic Sculpting of Directional Tuning in the Primate Motor Cortex during Three-Dimensional Reaching J. Neurosci., September 10, 2008; 28(37): 9164 - 9172. [Abstract] [Full Text] [PDF]

				S.-C. Yen, J. Baker, and C. M. Gray Heterogeneity in the Responses of Adjacent Neurons to Natural Stimuli in Cat Striate Cortex J Neurophysiol, February 1, 2007; 97(2): 1326 - 1341. [Abstract] [Full Text] [PDF]

				P. J. Magill, A. Pogosyan, A. Sharott, J. Csicsvari, J. P. Bolam, and P. Brown Changes in functional connectivity within the rat striatopallidal axis during global brain activation in vivo. J. Neurosci., June 7, 2006; 26(23): 6318 - 6329. [Abstract] [Full Text] [PDF]