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This Article Full Text Full Text (PDF) All Versions of this Article: 94/3/1928    most recent 00272.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Datta, S. Articles by Prutzman, S. L. Search for Related Content PubMed PubMed Citation Articles by Datta, S. Articles by Prutzman, S. L.

Novel Role of Brain Stem Pedunculopontine Tegmental Adenylyl Cyclase in the Regulation of Spontaneous REM Sleep in the Freely Moving Rat

Sleep and Cognitive Neuroscience Laboratory, Department of Psychiatry, Boston University School of Medicine, Boston, Massachusetts

Submitted 15 March 2005; accepted in final form 6 May 2005

Physiological activation of kainate receptors and GABA_B receptors within the pedunculopontine tegmentum (PPT) is involved in regulation of rapid-eye-movement (REM) sleep. Because these two types of receptors may also directly and/or indirectly activate the intracellular cyclic adenosine monophosphate (cAMP) signaling pathway, we hypothesized that this signaling pathway may be involved in the PPT to regulate spontaneous REM sleep. To test this hypothesis, four different doses (0.25, 0.50, 0.75, and 1.0 nmol) of a specific adenylyl cyclase (AC) inhibitor, 9-(tetrahydro-2-furanyl)-9H-purin-6-amine (SQ22536), were microinjected bilaterally (100 nl/site) into the PPT, and the effects on REM sleep in freely moving chronically instrumented rats were quantified. By comparing alterations in the patterns of REM sleep after control injections of vehicle or one of the four different doses of SQ22536, the contributions made by each dose of SQ22536 to REM sleep were evaluated. The results demonstrated that the local microinjection of AC inhibitor SQ22536 into the PPT decreased the total amount of REM sleep for 3 h and increased slow-wave sleep (SWS) for 2 h in a dose-dependent manner. This reduction in REM sleep was due to increased latency and decreased frequency of REM sleep episodes. These results provide evidence that inhibition of AC within the PPT can successfully reduce REM sleep. These findings suggest that activation of the cAMP-signaling pathway within the cholinergic cell compartment of the PPT is an intracellular biochemical/molecular step for generating REM sleep in the freely moving rat.

This article has been cited by other articles:

				S. Datta Activation of Pedunculopontine Tegmental PKA Prevents GABAB Receptor Activation-Mediated Rapid Eye Movement Sleep Suppression in the Freely Moving Rat J Neurophysiol, June 1, 2007; 97(6): 3841 - 3850. [Abstract] [Full Text] [PDF]

				R. S. Bandyopadhya, S. Datta, and S. Saha Activation of pedunculopontine tegmental protein kinase a: a mechanism for rapid eye movement sleep generation in the freely moving rat. J. Neurosci., August 30, 2006; 26(35): 8931 - 8942. [Abstract] [Full Text] [PDF]