JN Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 94: 2105-2110, 2005. First published June 15, 2005; doi:10.1152/jn.00414.2005
0022-3077/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
94/3/2105    most recent
00414.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ermilov, S. A.
Right arrow Articles by Anvari, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ermilov, S. A.
Right arrow Articles by Anvari, B.

Effects of Salicylate on Plasma Membrane Mechanics

Sergey A. Ermilov1, David R. Murdock1, Dania El-Daye1, William E. Brownell2 and Bahman Anvari1

1Department of Bioengineering, Rice University; and 2Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine, Houston, Texas

Submitted 22 April 2005; accepted in final form 7 June 2005

High concentrations of the amphipathic drug salicylate (Sal) block outer hair cell (OHC) electromotility resulting in reversible hearing loss. We used optical tweezers to study the effects of Sal on the mechanics of the cell plasma membrane. Membrane tethers were formed from guinea pig OHCs and cultured human embryonic kidney (HEK) cells as controls. HEK cells are commonly used in functional expression studies of electromotility. Effective tether viscosity ({eta}eff), steady-state tethering force extrapolated to zero pulling rate Fss(0), and time constant for tether growth ({tau}tg) were estimated from the measurements of the instantaneous tethering force at different tether pulling rates. Average values of {eta}eff, Fss(0), and {tau}tg for the OHC lateral wall plasma membrane and control cell plasma membrane remained the same after Sal perfusion, which is consistent with the hypothesis that Sal-induced reversible hearing loss appears to be more the result of its competition with essential anions and less the result of a change in plasma membrane mechanics.

Address for reprint requests and other correspondence: B. Anvari, Rice University, Department of Bioengineering, P.O. Box 1892, MS 142, Houston, TX 77251-1892 (E-mail: anvari{at}rice.edu)




This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
J. Ashmore
Cochlear Outer Hair Cell Motility
Physiol Rev, January 1, 2008; 88(1): 173 - 210.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
R. Zhang, F. Qian, L. Rajagopalan, F. A. Pereira, W. E. Brownell, and B. Anvari
Prestin Modulates Mechanics and Electromechanical Force of the Plasma Membrane
Biophys. J., July 1, 2007; 93(1): L07 - L09.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
I. Titushkin and M. Cho
Distinct Membrane Mechanical Properties of Human Mesenchymal Stem Cells Determined Using Laser Optical Tweezers
Biophys. J., April 1, 2006; 90(7): 2582 - 2591.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the The American Physiological Society.