HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 95/3/1949    most recent 00959.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Kay, A. R. Articles by Tóth, K. Search for Related Content PubMed PubMed Citation Articles by Kay, A. R. Articles by Tóth, K.

Influence of Location of a Fluorescent Zinc Probe in Brain Slices on Its Response to Synaptic Activation

¹Biological Sciences, University of Iowa, Iowa City, Iowa; and ²Centre de Recherche Robert Giffard and Department of Psychiatry, Université Laval, Quebec, Canada

Submitted 12 September 2005; accepted in final form 22 November 2005

The precise role of the high concentration of ionic zinc found in the synaptic vesicles of certain glutamatergic terminals is unknown. Fluorescent probes with their ability to detect ions at low concentrations provide a powerful approach to monitoring cellular Zn²⁺ levels. In the last few years, a number of fluorescent probes (indicators) have been synthesized that can be used to visualize Zn²⁺ in live cells. The interpretation of data gathered using such probes depends crucially on the location of the probe. Using acutely prepared hippocampal slices, we provide evidence that the Zn²⁺ probes, ZnAF-2 and ZP4, are membrane permeant and are able to pass into synaptic vesicles. In addition, we show that changes in fluorescence of the Zn²⁺ probes can be used to monitor presynaptic activity; however, these changes are inconsistent with Zn²⁺ release.

This article has been cited by other articles:

				A. R. Kay and K. Toth Is Zinc a Neuromodulator? Sci. Signal., May 13, 2008; 1(19): re3 - re3. [Abstract] [Full Text] [PDF]

				N. Lavoie, M. R. Peralta III, M. Chiasson, K. Lafortune, L. Pellegrini, L. Seress, and K. Toth Extracellular chelation of zinc does not affect hippocampal excitability and seizure-induced cell death in rats J. Physiol., January 1, 2007; 578(1): 275 - 289. [Abstract] [Full Text] [PDF]