HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 96/3/1053    most recent 01313.2005v2 01313.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Parnas, I. Articles by Parnas, H. Search for Related Content PubMed PubMed Citation Articles by Parnas, I. Articles by Parnas, H.

Role of NSF in Neurotransmitter Release: A Peptide Microinjection Study at the Crayfish Neuromuscular Junction

¹Department of Neurobiology, The Hebrew University, Jerusalem, Israel; and ²Department of Neurochemistry, Max-Planck Institute for Brain Research, Frankfurt am Main, Germany

Submitted 14 December 2005; accepted in final form 20 May 2006

Peptides that inhibit the SNAP-stimulated ATPase activity of N-ethylmaleimide-sensitive fusion protein (NSF-2, NSF-3) were injected intra-axonally to study the role of this protein in the release of glutamate at the crayfish neuromuscular junction. Macropatch recording was used to establish the quantal content and to construct synaptic delay histograms. NSF-2 or NSF-3 injection reduced the quantal content, evoked by either direct depolarization of a single release bouton or by axonal action potentials, on average by 66 ± 12% (mean ± SD; n = 32), but had no effect on the time course of release. NSF-2 had no effect on the amplitude or shape of the presynaptic action potential nor on the excitatory nerve terminal current. Neither NSF-2 nor NSF-3 affected the shape or amplitude of single quantal currents. Injection of a peptide with the same composition as NSF-2, but with a scrambled amino acid sequence, failed to alter the quantal content. We conclude that, at the crayfish neuromuscular junction, NSF-dependent reactions regulate quantal content without contributing to the presynaptic mechanisms that control the time course of release.

This article has been cited by other articles:

				Y. M. Kupchik, G. Rashkovan, L. Ohana, T. Keren-Raifman, N. Dascal, H. Parnas, and I. Parnas Molecular mechanisms that control initiation and termination of physiological depolarization-evoked transmitter release PNAS, March 18, 2008; 105(11): 4435 - 4440. [Abstract] [Full Text] [PDF]

				T. Kuner, Y. Li, K. R. Gee, L. F. Bonewald, and G. J. Augustine Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release PNAS, January 8, 2008; 105(1): 347 - 352. [Abstract] [Full Text] [PDF]