| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Brain-Body Institute, 2Department of Psychiatry and Behavioral Neurosciences, and 3Department of Medicine, McMaster University, Hamilton, Ontario, Canada
Submitted 25 February 2006; accepted in final form 11 April 2006
We recorded from myenteric AH/Dogiel type II cells, demonstrated mechanosensitive responses, and characterized their basic properties. Recordings were obtained using the mouse longitudinal muscle myenteric plexus preparation with patch-clamp and sharp intracellular electrodes. The neurons had an action potential hump and a slow afterhyperpolarization (AHP) current. The slow AHP was carried by intermediate conductance Ca2+-dependent K+-channel currents sensitive to charybdotoxin and clotrimazole. All possessed a hyperpolarization-activated current that was blocked by extracellular cesium. They also expressed a TTX-resistant Na+ current with an onset near the resting potential. Pressing on the ganglion containing the patched neuron evoked depolarizing potentials in 17/18 cells. The potentials persisted after synaptic transmission was blocked. Volleys of presynaptic electrical stimuli evoked slow excitatory postsynaptic potentials (EPSPs) in 9/11 sensory neurons, but 0/29 cells received fast EPSP input. The slow EPSP was generated by removal of a voltage-insensitive K+ current. Patch-clamp recording with a KMeSO4-containing, but not a conventional KCl-rich, intracellular solution reproduced the single-spike slow AHPs and low input resistances seen with sharp intracellular recording. Cell-attached recording of intermediate conductance potassium channels supported the conclusion that the single-spike slow AHP is an intrinsic property of intestinal AH/sensory neurons. Unitary current recordings also suggested that the slow AHP current probably does not contribute significantly to the high resting background conductance seen in these cells. The characterization of mouse myenteric sensory neurons opens the way for the study of their roles in normal and pathological physiology.
This article has been cited by other articles:
|
|
 |
|
  E. J. Dickson, G. W. Hennig, D. J. Heredia, H.-T. Lee, P. O. Bayguinov, N. J. Spencer, and T. K. Smith Polarized intrinsic neural reflexes in response to colonic elongation J. Physiol., September 1, 2008; 586(17): 4225 - 4240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nurgali, T. V. Nguyen, H. Matsuyama, M. Thacker, H. L. Robbins, and J. B. Furness Phenotypic changes of morphologically identified guinea-pig myenteric neurons following intestinal inflammation J. Physiol., September 1, 2007; 583(2): 593 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhu, J. Ye, and J. D. Huizinga Clotrimazole-sensitive K+ currents regulate pacemaker activity in interstitial cells of Cajal Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1715 - G1725. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. V. Nguyen, H. Matsuyama, J. Baell, B. Hunne, C. J. Fowler, J. E. Smith, K. Nurgali, and J. B. Furness Effects of Compounds That Influence IK (KCNN4) Channels on Afterhyperpolarizing Potentials, and Determination of IK Channel Sequence, in Guinea Pig Enteric Neurons J Neurophysiol, March 1, 2007; 97(3): 2024 - 2031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C Bornstein Intrinsic Sensory Neurons of Mouse Gut--Toward a Detailed Knowledge of Enteric Neural Circuitry Across Species. Focus on "Characterization of Myenteric Sensory Neurons in the Mouse Small Intestine" J Neurophysiol, September 1, 2006; 96(3): 973 - 974. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
