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This Article Full Text Full Text (PDF) All Versions of this Article: 96/5/2274    most recent 00328.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Jean-Xavier, C. Articles by Vinay, L. Search for Related Content PubMed PubMed Citation Articles by Jean-Xavier, C. Articles by Vinay, L.

Inhibitory Postsynaptic Potentials in Lumbar Motoneurons Remain Depolarizing After Neonatal Spinal Cord Transection in the Rat

Laboratoire Plasticité et Physio-Pathologie de la Motricité, Centre National de la Recherche Scientifique (CNRS) and Aix-Marseille Université, CNRS, Marseille, France

Submitted 29 March 2006; accepted in final form 26 June 2006

GABA and glycine are excitatory in the immature spinal cord and become inhibitory during development. The shift from depolarizing to hyperpolarizing inhibitory postsynaptic potentials (IPSPs) occurs during the perinatal period in the rat, a time window during which the projections from the brain stem reach the lumbar enlargement. In this study, we investigated the effects of suppressing influences of the brain on lumbar motoneurons during this critical period for the negative shift of the reversal potential of IPSPs (E_IPSP). The spinal cord was transected at the thoracic level on the day of birth [postnatal day 0 (P0)]. E_IPSP, at P4–P7, was significantly more depolarized in cord-transected than in cord-intact animals (E_IPSP above and below resting potential, respectively). E_IPSP at P4–P7 in cord-transected animals was close to E_IPSP at P0–P2. K-Cl cotransporter KCC2 immunohistochemistry revealed a developmental increase of staining in the area of lumbar motoneurons between P0 and P7 in cord-intact animals; this increase was not observed after spinal cord transection. The motoneurons recorded from cord-transected animals were less sensitive to the experimental manipulations aimed at testing the functionality of the KCC2 system, which is sensitive to [K⁺]_o and blocked by bumetanide. Although bumetanide significantly depolarized E_IPSP, the shift was less pronounced than in cord-intact animals. In addition, a reduction of [K⁺]_o affected E_IPSP significantly only in cord-intact animals. Therefore influences from the brain stem may play an essential role in the maturation of inhibitory synaptic transmission, possibly by upregulating KCC2 and its functionality.

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