Supplemental Movie

Movie Clip

Files in this Data Supplement:

• movie 1-3 - A file containing 3 movie clips (Quick-time format) is submitted separately on-line. Movie 1: A VZ wave with a circular trajectory, recorded from an E30 rabbit retina loaded with Fura 2-AM. The images are subtracted by a control image averaged from 4 frames immediately before the wave. The movie is played at 6x real speed. The frames are 1.15mm x 1.15mm. Movie 2: Movies of background-subtracted images from the VZ of Fluo4-AM loaded E29 retina, showing a VZ wave induced by KA puff at the GCL, followed by a spontaneous VZ wave. Notice that the second wave did not invade the boundary set by the preceding wave. The image contrast in this movie was inverted so that the dark regions indicate the areas of elevated intracellular free Ca2+ concentration. The frames are 1.15mm x 1.15mm, played back at 18x real speed. Movie 3: Simultaneous MEA recording from GCL and Ca2+ imaging from VZ of an E29 rabbit retina. The propagation of spikes in GCL (shown on the left) is played back together (at 6x real speed) with background-subtracted fluorescence images from the corresponding VZ area (on the right). The frames are 1.6mm x 1.6mm on the left and 1.15mm x 1.15mm on the right.