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Department of Physiology, Queen's University, Kingston, Ontario, Canada
Submitted 11 March 2008; accepted in final form 21 April 2008
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
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; Hille 2003). Changes in Ca2+ can be amplified or prolonged through Ca2+-induced Ca2+ release (CICR) from intracellular stores (Endo et al. 1970; Fabiato and Fabiato 1975). Neuronal CICR was first seen in Aplysia (Gorman and Thomas 1980), then confirmed in bullfrog (Lipscombe et al. 1988; Smith et al. 1983) and rat (Neering and McBurney 1984). CICR is typically considered the product of voltage-gated Ca2+ influx opening ryanodine receptors (RyRs) on the endoplasmic reticulum (ER) to cause Ca2+ release (Bardo et al. 2006; Verkhratsky 2005). CICR is sensitive to the alkyloid, ryanodine (Imagawa et al. 1987; Meissner 1985), as well as depletion of ER Ca2+ by the Ca2+-ATPase blocker, cyclopiazonic acid (CPA) (Seidler et al. 1989), or the RyR agonist, caffeine (Rousseau et al. 1988; Weber 1968).
On entry from the extracellular space or the ER, mitochondria may take Ca2+ up via the mitochondrial Ca2+ uniporter (Colegrove et al. 2000a,b; Gunter and Gunter 1994; Gunter and Pfeiffer 1990; Kim et al. 2005; Kirichok et al. 2004). After sequestering Ca2+, mitochondria can release it back into the cytosol, typically through Na+/Ca2+ and H+/Ca2+ exchangers (Colegrove et al. 2000a,b; Gunter and Pfeiffer 1990; Pauceka and Jab
rekb 2004; Puskin et al. 1976;Wingrove and Gunter 1986). Mitochondrial Ca2+ buffering and release is involved in post-tetanic potentiation (Tang and Zucker 1997; Zhong et al. 2001) and exocytosis (Billups and Forsythe 2002; Giovannucci et al. 1999). Moreover, while not traditionally considered as a source for CICR, mitochondria have been found to modulate CICR (Friel and Tsien 1994; Jackson and Thayer 2006). In the present study, we further this role considerably by suggesting that mitochondrial Ca2+ release is a prerequisite for CICR in the bag cell neurons of Aplysia.
The bag cell neurons from the marine mollusk, Aplysia californica, are neuroendocrine cells that control reproduction (Dudek et al. 1979; Kupfermann 1967; Kupfermann and Kandel 1970; Pinsker and Dudek 1977). Following brief synaptic input, these neurons undergo a 30-min afterdischarge that triggers neuropeptide release and initiates egg-laying behavior (Arch 1972a,b; Loechner et al. 1990; Stuart et al. 1980). The bag cell neurons have been employed to examine ion channels, peptide release, and intracellular Ca2+. For example, Fisher et al. (1994) used Ca2+-sensitive electrodes to show that Ba2+ entry caused Ca2+ release, suggestive of CICR. In addition, both Ca2+ entry and release in these neurons have been linked to the synthesis and secretion of neuropeptides (Berry and Arch 1981; Loechner et al. 1990; Michel and Wayne 2002). Here we demonstrate that bag cell neurons do in fact exhibit CICR and that the underlying mechanism involves store interaction, i.e., following Ca2+ influx, mitochondrial Ca2+ release stimulates the ER to release Ca2+. More comprehensively, neurons may use store interaction to translate episodes of short-term electrical activity into long-term changes in intracellular Ca2+, thereby activating membrane conductances as well as trigger secretion, transcription, or translation.
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METHODS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
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Primary cultures of isolated bag cell neurons were obtained from adult A. californica weighing 150–450 g. Animals were obtained from Marinus (Long Beach, CA) and housed in an 
300-l aquarium containing continuously circulating, aerated artificial seawater (Instant Ocean; Aquarian System; Mentor, OH) at 14–16°C on 12/12 h light/dark cycle and fed Romaine lettuce 3–5 times/wk. Following anesthesia by injection of isotonic MgCl2 (around 50% of body weight), the abdominal ganglion was removed and treated for 18 h with neutral protease (13.33 mg/ml; 165859; Roche Diagnostics, Indianapolis, IN) dissolved in tissue culture artificial seawater (tcASW; containing in mM): 460 NaCl, 10.4 KCl, 11 CaCl2, 55 MgCl2, and 10 HEPES, pH 7.8 supplemented with glucose (1 mg/ml), penicillin (100 u/ml), and streptomycin (0.1 mg/ml). The ganglia were then transferred to fresh tcASW, and the two bag cell neuron clusters were dissected from their surrounding connective tissue. Using a fire-polished Pasteur pipette and gentle trituration, neurons were dispersed in tcASW onto regular 35 x 10 mm polystyrene tissue culture dishes (430165; Corning, Corning, NY). Cultures were maintained in tcASW for 1–3 days in a 14°C incubator. Experiments were carried out at 22°C in normal ASW (nASW; composition same as tcASW but with glucose and antibiotics omitted). Salts were obtained from Fisher Scientific (Ottawa, ON, Canada) or Sigma-Aldrich (St. Louis, MO).
Sharp-electrode current-clamp recording
Current-clamp recordings were made using an Axoclamp 2B amplifier (Axon Instruments/ Molecular Devices, Sunnyvale, CA) in bridge mode and the sharp-electrode method. Microelectrodes were pulled from 1.2 mm ID, boroscilicate glass capillaries (IB120F-4; World Precision Instruments, Sarasota, FL), with a resistance of 7–12 M
when filled with 2 M K-acetate (supplemented with 100 mM KCl and 10 mM HEPES, pH = 7.3 with KOH). Voltage signals were filtered at 3 kHz and sampled at 2 kHz using an IBM-compatible personal computer, a Digidata 1300 A/D converter (Axon Instruments) and the Clampex acquisition program of pCLAMP 8.1 (Axon Instruments). Stimulation current was delivered using a S88 stimulator (Grass, Warwick, MA). Electrophysiology was typically carried out simultaneously with Ca2+ imaging.
Ca2+ imaging
The Ca2+-sensitive dye, fura-PE3 (K+ salt; 0110; Teflabs, Austin, TX) (Vorndran et al. 1995), was pressure injected via sharp-electrode using a PMI-100 pressure microinjector (Dagan, Minneapolis, MN), while simultaneously monitoring membrane potential with the Axoclamp. Microelectrodes (as per sharp-electrode current-clamp) had a resistance of 15–30 M when the tip was filled with 10 mM fura-PE3 then backfilled with 3 M KCl. Injections required 3–10 0.2-ms pulses at 50–100 kPa to fill the neurons with an optimal amount of dye-estimated to be 50–100 µM. All neurons used subsequently for imaging showed resting potentials of –50 to –60 mV and displayed action potentials that overshot 0 mV following depolarizing current injection (0.5–1 nA, directly from the amplifier). After dye injection, neurons were allowed to equilibrate for 
30 min. Imaging was performed using a Nikon TS100-F inverted microscope (Nikon, Mississauga, ON, Canada) equipped with Nikon Plan Fluor x20 (numerical aperture (NA) = 0.5) or x40 (NA = 0.6) objectives.The light source was a 75 W Xenon arc lamp and a multi-wavelength DeltaRAM V monochromatic illuminator (Photon Technology International, London, ON, Canada) coupled to the microscope with a UV-grade liquid-light guide. Excitation wavelengths were 340 and 380 nm. Between acquisition episodes, the excitation illumination was blocked by a shutter, which along with the excitation wavelength, was controlled by an IBM-compatible personal computer, a Photon Technology International computer interface, and ImageMaster Pro software (version 1.49, Photon Technology International). The emitted light passed through a 510/40 nm barrier filter prior to being detected by a Photon Technology International IC200 intensified charge coupled device camera. The camera intensifier voltage was set based on the initial fluorescence intensity of the cells at the beginning of each experiment and maintained constant thereafter. The camera black level was set prior to an experiment using the camera controller such that, at a gain of 1, there was a 50:50 distribution of blue and black pixels on the image display with no light going to the camera. Fluorescence intensities were sampled at 10-, 20-, or 60-s intervals using regions of interest (ROIs) defined over the neuronal somata prior to the start of the experiment and, if necessary, averaged four to eight frames per acquisition. The emission following 340 and 380 nm excitation was ratioed (340/380) to reflect free intracellular Ca2+ and saved for subsequent analysis. The black level determination, image acquisition, frame averaging, emitted light ROI sampling, and ratio calculations were carried out using the ImageMaster Pro software.
Reagents and drug application
CPA (C1530; Sigma-Aldrich or 239805; Calbiochem, San Diego, CA), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP; 21857; Sigma-Aldrich, ryanodine (559276; Calbiochem), and phorbol 12-myristate 13-acetate (PMA; P8139; Sigma-Aldrich) all required dimethyl sulfoxide (DMSO; BP231; Fisher) as a vehicle. The maximal final concentration of DMSO was 0.01 µM, which in control experiments had no effect on resting intracellular Ca2+ or Ca2+ transients evoked by action potential trains. The lack of an effect of DMSO is consistent with prior work by ourselves (Kachoei et al. 2006) and others (Jonas et al. 1997; Knox et al. 1992, 1996, 2004), who employ the bag cell neurons to study Ca2+ or Ca2+ channels. Tetraphenylphosphonium chloride (TTP; 218790; Sigma-Aldrich) was made up in water. Drug application or solution exchanges were accomplished by manual perfusion using a calibrated transfer pipette to exchange the bath (tissue culture dish) solution. In most cases, drugs were introduced directly into the bath by pipetting a small volume (<10 µl) of concentrated stock solution or a larger volume of saline (100 µl) that was initially removed from the bath, mixed with the stock solution, and then reintroduced. Care was taken to perform all pipetting near the side of the dish and as far away as possible from the neurons. Pretreatment duration was 20–30 min unless stated otherwise.
Analysis
Origin (version 7; OriginLab, Northampton, MA) was used to import and plot ImageMaster Pro files as line graphs. For intracellular Ca2+, analysis usually compared the steady-state value of the baseline 340/380 ratio with the ratio from regions that had reached a peak or new steady-state during either action potential firing or CICR. For CICR, these regions are indicted in the figures by short dotted lines. Averages of both regions were determined by eye or with adjacent-averaging. Change was expressed as a percent change (peak % 
340/380 or CICR % 340/380) of the new ratio over the baseline ratio. In one instance (Fig. 7C), the absolute change in the 340/380 during action potential firing was calculated. The time course of recovery for the Ca2+ signal was quantified by determining the time required, after delivery of the stimulus, for the 340/380 ratio to return to 75% of the baseline ratio observed before the stimulus. Summary data are presented as the means ± SE. Statistics were performed using Instat (version 3.0; GraphPad Software, San Diego, CA). The Kolmogorov-Smirnov method was used to test data sets for normality. If the data were normal, Student's paired or unpaired (with the Welch correction as required) t-test was used to test for differences between two means, while a standard one-way ANOVA with Dunnett's post hoc test were used to test for differences between multiple means. If the data were not normally distributed, a Mann-Whitney test was used to test for differences between two means, while a Kruskal-Wallis ANOVA and Dunn's post hoc test were used to test for differences between multiple means. Data were considered significantly different if the two-tailed P value was <0.05.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
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Previous studies of action potential-induced Ca2+-influx in cultured bag cell neurons showed that both single spikes and bursts elevated intracellular Ca2+ (Fink et al. 1988; Fisher et al. 1994; Knox et al. 1992). However, in none of these cases were the rates and duration of the stimulus controlled to reflect physiological firing rates. Typically, the afterdischarge in the intact cluster is evoked by a 4- to 6-Hz, 10-s train, which then results in two phases of action potential firing: a fast phase of 1 min at 2–6 Hz, followed by a slow phase of close to 30 min at 0.5–1 Hz (Fisher et al. 1994; Kaczmarek et al. 1982; Magoski and Kaczmarek 2005; Zhang et al. 2002). Thus for the present study, changes to intracellular Ca2+ were evoked with either a short train of action potentials corresponding to the stimulus used to trigger the afterdischarge or a long train corresponding to the fast phase of the afterdischarge itself. In either case, we sought to determine if the Ca2+ rise produced by the stimulus resulted in subsequent intracellular Ca2+ release.
The short train of action potentials was delivered at 5 Hz for 10 s to individual cultured bag cell neurons current-clamped to –60 mV. This elicited a distinct, fast-rising episode of Ca2+ influx, followed by a slow, essentially monoexponential decay back to baseline over the course of a few minutes (Fig. 1 A; n = 14). An endoplasmic reticulum Ca2+-ATPase blocker, CPA, was added before delivering the train to determine if the Ca2+ elevation was influenced by ER Ca2+ uptake and/or release. Following the 5-Hz, 10-s train, the peak percent change in Ca2+ for neurons treated with 20 µM CPA was not statistically different from control (Fig. 1, B and C; n = 10). Similarly the time to return to 75% of baseline Ca2+, following administration of the train, was not significantly affected by CPA (Fig. 1D).
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). An immediate and robust Ca2+ influx was observed with the delivery of this stimulus, followed by a slower, prolonged Ca2+ elevation (Fig. 2 A; n = 6). This subsequent increase lasted several minutes and showed a distinct plateau suggestive of CICR. A hallmark of CICR is that Ca2+ influx subsequently opens RyRs on the ER, thereby causing a secondary Ca2+ rise. A potential role for the RyR-ER pathway in releasing and sustaining Ca2+ was investigated by blocking RyRs prior to delivering the 5-Hz, 1-min train. Pretreatment with 100 µM ryanodine significantly attenuated the amplitude of CICR by almost 2/3 when compared with control (Fig. 2, B and C; n = 5) without affecting the time to 75% recovery (data not shown).
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The role of the ER in releasing and sustaining Ca2+ during CICR was further investigated with the addition of 20 µM CPA prior to the 5-Hz, 1-min train of action potentials. Delivery of the stimulus to untreated neurons evoked characteristic rapid influx and subsequent CICR (Fig. 3 A; n = 17). However, as a result of CPA-induced depletion, the amplitude of CICR was significantly attenuated by nearly half when compared with control (Fig. 3, B and C; n = 7). The time course of recovery to 75% of basal Ca2+ was not altered (data not shown).
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; Neering and McBurney 1984; Orkand and Thomas 1995; Shmigol et al. 1995; Solovyova et al. 2002; Usachev and Thayer 1997), it was not employed in the present study. If applied at concentrations required to deplete the ER (in the mM range), caffeine strongly depolarizes bag cell neuron resting potential and inhibits voltage-dependent K+ channels (N. S. Magoski, unpublished results). Moreover, many bag cell neuron conductances are sensitive to adenosine 3':5'-cyclic monophosphate (cAMP) (Kazcmarek and Strumwasser 1984), and the inhibitory actions of caffeine on phosphodiesterases (Beavo et al. 1970) would confound matters by elevating cAMP levels. Collapse of the mitochondrial membrane potential largely eliminates CICR
Although not considered requisite for CICR, other reports have suggested that mitochondrial buffering or release of Ca2+ can influence CICR (Friel and Tsien 1994; Jackson and Thayer 2006; Werth and Thayer 1994). Thus, the role of mitochondrial stores in bag cell neuron CICR was examined by applying FCCP prior to delivering the stimulus. FCCP is a protonophore that collapses the mitochondrial membrane potential and both depletes the organelle of Ca2+ and prevents subsequent Ca2+ release from the store (Collins et al. 2000; Heytler and Prichard 1962; Simpson and Russell 1996). As expected, pretreatment with FCCP before the stimulus resulted in robust Ca2+ elevation. Moreover in preliminary experiments, FCCP was seen to evoke a prominent depolarization coincident with depletion of mitochondrial Ca2+. We have since learned that this depolarization appears to be caused by Ca2+-dependent activation of a nonselective cation current (C. M. Hickey, J. E. Geiger, and N. S. Magoski, unpublished data). For all experiments included here, the FCCP-induced depolarization was counteracted manually by increasing, and later decreasing, delivery of hyperpolarizing current, such that the membrane potential was maintained at –60 mV throughout.
While delivery of a 5-Hz, 1-min train from –60 mV resulted in a robust Ca2+ influx and CICR in nASW (Fig. 4 A; n = 7), pretreatment with 20 µM FCCP significantly reduced CICR amplitude to one-fifth of the control amplitude (Fig. 4, B and E; n = 12). The time required to reach 75% recovery to baseline Ca2+ was significantly shortened by FCCP (Fig. 4D). Provided CPA and FCCP act on separate Ca2+ stores (see next section), both of which appear to be involved in CICR, the response to the long train was examined following dual depletion. As was done before, neurons were pretreated with 20 µM CPA to deplete the ER store. Once the fluorescence ratio intensity returned to baseline (20–40 min), FCCP was applied and the membrane potential was maintained at –60 mV. Subsequent to dual depletion, the 5-Hz, 1-min train was delivered with the result being a clear and significant reduction in CICR (Fig. 4C; n = 6). The effects of both drugs together, however, were not additive, and compared with control, the combined CPA/FCCP treatment was no more effective than FCCP alone (Fig. 4E).
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Key to suggesting that the effects of CPA and FCCP are disparate is the certainty that they target separate Ca2+ stores. To confirm the ability of CPA to effectively deplete the ER, 20 µM CPA was added to fura-loaded neurons in Ca2+-free ASW. With no Ca2+ present in the bath, CPA addition resulted in a rise in Ca2+ followed by a return to near baseline. With subsequent addition of CaCl2 to the bath, a second prominent rise in Ca2+ was observed (Fig. 5 A; n = 11). This second Ca2+ elevation is referred to as store-operated Ca2+ influx, a processes observed in a diversity of cell types and species. Store-operated Ca2+ influx only occurs when the ER has been depleted of Ca2+, resulting in a signal to the plasma membrane that opens Ca2+-selective channels to replete the store (Putney 2003). Our laboratory has previously confirmed that bag cell neuron store-operated Ca2+ influx is pharmacologically and functionally the same as other systems (Kachoei et al. 2006). To determine whether the effects of CPA and FCCP depletion could be distinguished from one another, both agents were added in succession in Ca2+-free ASW. After depleting the ER stores with 20 µM CPA and allowing recovery to near baseline Ca2+, 20 µM FCCP was added to the bath. The introduction of FCCP resulted in a second elevation of Ca2+ (Fig. 5B; n = 13), thus signifying that the ER and the mitochondria are distinct Ca2+ stores.
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FCCP decreases ATP synthesis as a result of dissipating the mitochondrial membrane potential (Abas et al. 2000; Allemann and Schneider 2000). Moreover, lowered ATP levels can reduce CICR (Abas et al. 2000; Murayama et al. 2000), presumably because of a loss of the direct stimulatory effect of ATP on the RyR channel (Rousseau et al. 1986). To rule out the possibility that a reduction in ATP was involved in decreasing CICR amplitude, the mitochondrial Ca2+ release blocker, tetraphenylphosphonium (TPP), was used. Unlike FCCP, TPP hinders mitochondrial Ca2+ release without affecting ATP production (Aiuchi et al. 1985). Specifically, TPP inhibits both the Na+/Ca2+ and H+/Ca2+ exchanger on the mitochondrial membrane and thus blocks Ca2+ release without depleting the mitochondria of Ca2+ (Karodjov et al. 1986; Wingrove and Gunter 1986).
In control cells, delivery of the 5-Hz, 1-min train triggered substantial Ca2+ influx and CICR (Fig. 6 A; n = 13). Consistent with other reports (Tang and Zucker 1997), pretreatment with 100 µM TPP did not change the membrane potential or intracellular Ca2+. However, in the presence of TPP, there was a significant reduction of CICR amplitude by nearly three-quarters with only negligible effects on peak Ca2+ influx (Fig. 6B; n = 5). TPP did not significantly change the duration of CICR (Fig. 6D) despite a FCCP-like trend in the group data toward shortened recovery time. Depletion of the ER store by 20 µM CPA, followed by 100 µM TPP treatment, also significantly attenuated CICR amplitude (Fig. 6C; n = 11). That said, the decrease resulting from combined CPA/TPP treatment was not significantly different from TPP alone (Fig. 6E). Thus as was the case for FCCP, elimination of the mitochondrial store effectively eliminated CICR. We also attempted to use KBR 7943, a different Ca2+ exchanger antagonist (Iwamoto et al. 1996); however, this drug blocked bag cell neuron Ca2+ current and prevented the initial influx.
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Approximately 5 min following the onset of the afterdischarge in intact bag cell neurons, protein kinase C (PKC) activity is upregulated (Wayne et al. 1999), which in turn enhances the voltage-gated Ca2+ current (Conn et al. 1989a,b; DeReimer et al. 1985; Strong et al. 1987). Consequently, we tested the effects of phorbol 12-myristate 13-acetate (PMA), an effective PKC activator in bag cell neurons (Castagna et al. 1982; Sossin and Schwartz 1994), on CICR. Delivery of the 5-Hz, 1-min train of action potentials in untreated neurons induced robust Ca2+ influx and CICR (Fig. 7A; n = 7). In contrast, neurons pretreated with 100 nM PMA for 25 min prior to the stimulus showed an approximately one-quarter reduction in peak Ca2+ influx during the train and no change in CICR amplitude (Fig. 7, B–D; n = 10). The Ca2+ influx during the train could have been lowered because of Ca2+-dependent inactivation of the Ca2+ current (Tillotson 1979). This form of use-dependent inactivation may have been increased due to a combination of the rapid stimulation and PKC-mediated enhancement of the Ca2+ current itself.
Changes to Ca2+ during a simulated afterdischarge
In examining the responses to the 5-Hz, 1-min train, the Ca2+ dynamics associated with the fast phase of the afterdischarge were studied in isolation. However, in vivo the fast phase is followed by a prolonged period of slow firing (around 1 Hz for 30 min). Moreover, in the prior experiment involving PKC activation, PMA was added well before the train was delivered. Yet in the intact cluster, PKC activity is not upregulated until 5 min subsequent to the onset of the afterdischarge (Wayne et al. 1999). Thus in an effort to recapitulate an afterdischarge-like stimulus, a compound train consisting of a 5-Hz, 1-min train (to mimic the fast phase), immediately followed by a 1-Hz, 30-min train (to mimic the slow phase) was delivered. Although the in vivo afterdisharge has a depolarizing component, we confined the stimulus to mimicking just action potential firing, so to limit both the extent of Ca2+ current inactivation (which would speed up if evoked repeatedly from a more depolarized potential) and the activation of any persistent voltage-dependent Ca2+ or cation channels (which would provide additional, uncontrolled Ca2+ influx). In addition, PMA was applied to some neurons just 10 min prior to the compound train, such that the kinetics for PKC activation would be more physiological. Conn et al. (1989b) showed that bath-applied PMA increased Ca2+ current in cultured bag cell neurons within 10–15 min.
Delivery of the simulated afterdischarge produced a biphasic Ca2+ elevation. This included an initial spike of Ca2+ during the 5-Hz, 1-min phase, followed by a slowly declining plateau through the course of the 1-Hz, 30-min phase (Fig. 8 A; n = 10). The Ca2+ elevation was quantified by taking the percentage change from baseline over the early and late periods of the 1-Hz, 30-min phase. On termination of the compound train, the Ca2+ returned to baseline relatively quickly, with a time to 75% recovery of close to 5 min (Fig. 8E). Remarkably, this appeared to be similar to, or even faster than, that seen following CICR induced by the 5-Hz, 1-min train alone (compare with Figs. 4D or 6D). Treatment with 100 nM PMA 10 min prior to the compound train significantly enhanced the Ca2+ elevation (Fig. 8B; n = 7). In comparison to control, the Ca2+ change during both the early and late period of the 30-min, 1-Hz phase was nearly a third-again as large with PMA. Moreover, PKC activation significantly prolonged the return to baseline duration, with almost a fourfold increase in the time to 75% recovery (Fig. 8E).
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
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; Richter et al. 2005; Shmigol et al. 1995; Smith et al. 1983) but is unlike other versions where brief firing or single action potentials are sufficient to initiate CICR (Cohen et al. 1997; Gorman and Thomas 1980; Hua et al. 1993; Orkand and Thomas 1995; Solovyova et al. 2002). Because the response in bag cell neurons occurs only subsequent to the fast phase-like train, it would suggest that Ca2+ release is more likely once the afterdischarge itself is underway rather than just after the initial stimulation. This may serve to avoid erroneous Ca2+ release.
In part, bag cell neuron CICR is consistent with similar phenomena observed in other preparations. First, it is reduced by ryanodine, which acts on RyRs found on the ER (Bardo et al. 2006; Berridge 1998; Meldolesi 2001). Typically between 50 and 100 µM is sufficient to eliminate CICR as reported in leech (Trueta et al. 2004), salamander (Suryanarayanan and Slaughter 2006), rat dorsal root ganglion (DRG) (Shmigol et al. 1995), frog sympathetic (Hua et al. 1993), and Hermissenda (Kawai et al. 2004) neurons. The present result is in agreement with RyRs playing a role in generating bag cell neuron CICR. Second, depletion of CPA-sensitive stores also reduces CICR in a manner much like that of ryanodine. This is further indicative of a RyR-ER pathway and is similar to that observed in Helix (Orkand and Thomas 1995), rat DRG (Usachev and Thayer 1997), rat hippocampal (Emptage et al. 1999), and rat thalamic (Richter et al. 2005) neurons. Nevertheless, given that bag cell neuron CICR is only partially blocked by either a high concentration of ryanodine or ER depletion, it suggests a secondary mechanism may be at work. It is unlikely that either ryanodine or CPA impact other Ca2+ pumps or exchangers in a nonspecific manner. For example, ryanodine does not alter basal cytoplasmic Ca2+ in our preparation or others (Hua et al. 1993; Ivanenko et al. 1993; Kang and Holz 2003), and CPA does not block the plasma membrane Ca2+-ATPase (Seidler et al. 1989). Similarly we are confident that the bag cell neuron ER is actually depleted by CPA because not only does it result in store-operated Ca2+ influx, but it also eliminates IP3-induced Ca2+ elevations (Jonas et al. 1997) and liberates the Ca2+ that remains in the ER after RyR activation by other agonists (Kachoei et al. 2006).
Bag cell neuron CICR is unique in that it is nearly eliminated by depletion of the mitochondrial store with FCCP, or preventing mitochondrial Ca2+ release with TPP. Moreover, simultaneous ER depletion by CPA does not reduce CICR further. Thus, although supported by Ca2+ from the ER, CICR appears to depend on mitochondrial Ca2+. FCCP both depletes mitochondria of Ca2+ and lowers oxidative phosphorylation by collapsing the mitochondrial membrane potential (Collins et al. 2000; Heytler and Prichard 1962). Because a decrease in ATP levels can inhibit the RyR channel (Rousseau et al. 1986), FCCP could suppress CICR indirectly. To eliminate this uncertainty, TPP, which blocks Ca2+ release from mitochondria (Wingrove and Gunter 1986) with little effect on ATP production (Aiuchi et al. 1985), was employed. TPP also appears to be specific, as it does not change basal Ca2+ or membrane potential in our preparation or the presynaptic terminals of crayfish motor neurons (Tang and Zucker 1997) and rat Caylx of Held (Billups and Forsythe 2002); this might be the case if it blocked Ca2+ pumps or exchangers not on the mitochondria. For bag cell neurons, the FCCP and TPP results are essentially identical and suggest that mitochondrial Ca2+ release is required for CICR.
There is evidence from other preparations that mitochondria can influence CICR. For example, when Werth and Thayer (1994) uncoupled mitochondria and prevented electron transport, it blocked depolarization-induced Ca2+ release in rat DRG neurons. They suggest that mitochondria exclusively take up Ca2+ as it enters during the depolarization and then release it during a plateau phase. Similarly, Friel and Tsien (1994) showed in frog sympathetic neurons that high K+ caused Ca2+ influx, presumed mitochondrial filling, and subsequent mitochondrial release of Ca2+ in a CICR-like fashion. This sustained Ca2+ release was prevented by FCCP but not altered by ryanodine or ER depletion with thapsigargin (Colegrove et al. 2000a; Jackson et al. 1988). Friel and Tsien (1992) also observed true CICR in these neurons, which was blocked by depletion with caffeine. Essentially, these studies concluded that a large Ca2+ influx engaged mitochondria, while a modest Ca2+ influx engaged ER with no interaction between the two.
However, bag cell neuron CICR hinges on the mitochondria, which raises the possibility of an interaction between mitochondrial and ER stores, as proposed by Jackson and Thayer (2006). They showed that both ATP and Ca2+ released from mitochondria influence the frequency and magnitude of caffeine-induced, RyR-dependent Ca2+ oscillations in rat DRG neurons. But is this the case for CICR in bag cell neurons? There are two plausible explanations for the apparent dependence of bag cell neuron CICR on mitochondria. First, Ca2+ influx during the stimulus may load the mitochondria with Ca2+, which would be subsequently released through one or both exchanger pathways. This Ca2+ would then activate RyRs, which in turn causes Ca2+ release from the ER (CPA and ryanodine sensitive). Depletion of the ER (with CPA) or blocking RyRs (with ryanodine) does not eliminate CICR because mitochondrial release would also contribute to the plateau. However, depletion of mitochondrial Ca2+ (with FCCP) or elimination of its release (with TPP) impedes both mechanisms. Second, Ca2+ influx during the 5-Hz, 1-min train could simply activate, through a currently unknown mechanism, the mitochondrial exchangers, which would release Ca2+ and initiate CICR as described.
FCCP may have changed bag cell neuron ATP levels, which could in turn affect RyRs or other ATP- or proton-dependent mechanisms, but this is not supported by the TPP result. Furthermore, FCCP-treated neurons recover from the 5-Hz, 1-min train-induced Ca2+ influx in a manner similar to controls, suggesting a nonspecific effect on Ca2+ handling is unlikely. Thus, although similar to that in rats and frogs, CICR in bag cell neurons is novel in that it appears to have absolute dependence on mitochondria. CICR is initiated and supported by Ca2+ buffering and release from the mitochondria with a subsequent contribution from the ER but without ATP-RyR interactions. This represents a departure from the classic model of CICR where Ca2+ influx activates RyRs directly and the mitochondria merely buffer Ca2+ entry (Fig. 9).
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). However, during the slow phase of the afterdischarge, PKC is activated, and a larger-conductance, PKC-sensitive channel is inserted into the plasma membrane (Conn et al. 1989b; DeRiemer et al. 1985; Strong et al. 1987; Wayne et al. 1999; White and Kaczmarek 1997; Zhang et al. 2008). Attempts at enhancing Ca2+ influx during the fast phase alone with PMA pretreatment were unsuccessful. This may have been due to a combination of greater initial Ca2+ and the high firing rate causing more Ca2+-dependent inactivation of the Ca2+ current and less overall Ca2+ influx. In many respects, this protocol fails to replicate natural phenomena; thus, PMA application was timed to elevate PKC activity several minutes into the slow phase of a compound train that mimicked the firing frequency of the afterdischarge. PKC activation resulted in a prominent increase in the overall amount of Ca2+ during the slow phase. Moreover, post-train recovery time was clearly lengthened by PMA as compared with control. This suggests that CICR may have occurred subsequent to the compound train. Other factors contributing to this prolonged post-compound train signal include the larger Ca2+ load from PKC-dependent upregulation of the Ca2+ current during the slow phase and possible effects of PKC on Ca2+ removal (Usachev et al. 2006).
That bag cell neurons have voltage-gated Ca2+ influx renders CICR somewhat enigmatic. Possibly CICR is needed to amplify or intensify peptide secretion. For example, CICR augments acetylcholine release from Aplysia buccal neurons (Mothet et al. 1998), serotonin from leech Retzius neurons (Trueta et al. 2004), glutamate from salamander photoreceptors (Suryanarayanan and Slaughter 2006), and insulin from a beta cell line (Kang and Holz 2003). Moreover Michel and Wayne (2002) found that bag cell neuron peptide secretion could persist well beyond the end of the afterdischarge. Perhaps this is due to the very prolonged recovery of intracellular Ca2+ we observed after the compound train in PMA-treated neurons. Postulations aside, bag cell neuron CICR should be considered in the context that our recordings were made from single neurons in culture at room temperature. In vivo these cells are electrically coupled to one another and typically operate at several degrees below room temperature. Thus the kinetics of Ca2+ influx, release, and handling may not be identical in the intact animal.
Bag cell neuron CICR may also activate membrane conductances contributing to the depolarizing drive for the afterdischarge, such as Ca2+-dependent, nonselective cation channels (Hung and Magoski 2007; Lupinsky and Magoski 2006; Magoski et al. 2002). This would be important during the slow phase, when voltage-gated Ca2+ influx is lessened. Our laboratory has observed cation channel activation subsequent to mitochondrial Ca2+ release (C. M. Hickey, J. E. Geiger, and N. S. Magoski, unpublished data), the latter of which appears to be critical for CICR. In chick DRG neurons, CICR activates Cl– channels (Ivanenko et al. 1993), while in rat hippocampal neurons, RyR blockade attenuates cation channel-mediated depolarization (Partridge and Valenzuela 1999).
Finally, CICR may impact several forms of Ca2+-dependent plasticity occurring during the afterdischarge. Such changes include the induction of the refractory period (Kaczmarek and Kauer 1983; Kaczmarek et al. 1982; Magoski et al. 2000) and enhanced translation of peptide hormone (Berry and Arch 1981; Wayne et al. 2004). These events may be mediated by CaM-kinase, which has increased activity during the afterdischarge (DeRiemer et al. 1984). CICR influences plasticity in other systems; for example, conditioning-mediated changes to Hermissenda photoreceptor excitability and morphology are RyR-dependent (Blackwell and Alkon 1999; Kawai et al. 2004). In hippocampal dendrites, CICR triggered by Ca2+ influx through N-methyl-D-aspartate receptors, has a role in inducing some forms of long-term potentiation and depression (Emptage et al. 1999; Harvey and Collingridge 1992; Reyes and Stanton 1996). Thus, there is the potential for mitochondrial/ER-dependent CICR in bag cell neurons to augment neurosecretion, enhance the depolarization necessary for secretion, or trigger plasticity.
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Address for reprint requests and other correspondence: N. S. Magoski, Dept. of Physiology, Queen's University, 4th Floor, Botterell Hall, 18 Stuart St., Kingston, ON K7L 3N6, Canada (E-mail: magoski{at}post.queensu.ca)
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). Baseline 340/380 is 0.392. The fluorescence ratio scale applies to A and B. In this and all subsequent Ca2+ traces concerning CICR, ··· indicates the region used to calculate the percent change in 340/380 as compared with the baseline ratio (see METHODS). B: following treatment with 100 µM ryanodine, the second phase of the Ca2+ elevation is markedly reduced. Baseline 340/380 is 0.303. C: summary graph indicating that prior application of ryanodine (Ryn) significantly attenuates CICR amplitude (unpaired t-test).
), 10 min prior to the delivery of the compound train. PMA results in a greater initial Ca2+ elevation, increased Ca2+ during both the early and late periods of the slow phase, less of a decline during the course of the slow phase, and a much longer time to recovery after the train. A short, spontaneous burst of action potentials occurs 