The Journal of Neurophysiology Vol. 82 No. 1 July 1999
CORRIGENDUM
Volume 81, April 1999
Pages 1636-1644: H. R. Koerber, K. Mirnics, A. M. Kavookjian, and
A. R. Light, "Ultrastructural Analysis of Ectopic Synaptic Boutons
Arising From Peripherally Regenerated Primary Afferent Fibers."
The reproduction quality of the micrographs shown in the original
printing was unsatisfactory. All of the micrographs are reproduced here
displaying a sharper
contrast.
View larger version (141K):
[in this window]
[in a new window]
|
Fig. 3.
Examples of 3 sections through the same bouton (fiber 2).
A: noncounterstained section through HRP-labeled
profile. Arrow shows asymmetric synaptic specialization.
B: section through the same profile counterstained with
uranyl acetate and lead citrate. Arrow shows same axo-dendritic
contact. C: high-power view of a 3rd section through the
same profile showing a 2nd axo-dendritic (long arrow) and an asymmetric
axo-axonic (short arrow) contact. Note numerous mitochondria contained
in the terminal. Scale bars, 0.5 µm; d, dendrite; at, axon
terminal.
|
|
View larger version (175K):
[in this window]
[in a new window]
|
Fig. 4.
Counterstained section through an ectopic bouton from fiber 1.  , asymmetric contact with a dendritic (d) profile that
contains a few clear round vesicles. Note close apposition between axon
terminals (at) containing pleomorphic vesicles and the HRP-stained
profile. Scale bar = 0.5 µm.
|
|
View larger version (150K):
[in this window]
[in a new window]
|
Fig. 5.
Additional examples of frequent synaptic contacts of HRP-stained
ectopic profiles. A and B: adjacent
sections through a bouton from fiber 2. Long arrows denote an
asymmetric contact with a dendritic profile (d) and short arrows point
to an asymmetric contact with a presynaptic axon terminal (at)
containing pleomorphic vesicles. C: section through a
different profile also from fiber 2 demonstrating similar
ultrastructure. Scale bar = 0.5 µm.
|
|
View larger version (149K):
[in this window]
[in a new window]
|
Fig. 6.
A: light micrograph of 100-µm-thick sagittal section.
Asterisk, locations of ectopic boutons (fiber 2) isolated for EM
analysis. B: example of a typical ectopic profile
forming an asymmetric axo-denritic contact (curved arrow). Profile is
filled with numerous clear round vesicles and a few large dense core
vesicles (arrows). Different arrows distinguish light and dark dense
core vesicles in the ectopic profile and surrounding neuropil.
C: example of a stained unmyelinated fiber filled with
clear round vesicles. Open arrow denotes an en passant bouton.
D: high-power view of same section. Scale bars:
A = 100 µm; B and
D = 0.5 µm; C = 1.0 µm.
|
|
View larger version (160K):
[in this window]
[in a new window]
|
Fig. 7.
Example of similar ultrastructure seen in Fig. 6, C and
D, for a bouton from fiber 1. A: section
through a bouton and unmyelinated fiber. , asymmetric
axo-dendritic contact. Note numerous clear round vesicles in the fiber.
B: enlargement of same section. Scale bars = 0.5 µm.
|
|
