| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030; 2 Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030
Submitted 8 April 2003; accepted in final form 7 May 2003
 |
ABSTRACT |
|---|
 TOP ABSTRACT INTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
|
INTRODUCTION |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
). A large number of studies show that several protein kinases are critical in the modulation of nociception. In particular, strong evidence implicates protein kinase C (PKC), protein kinase A (PKA), and the extracellular signal-regulated kinases (ERKs) in the establishment and/or maintenance of central sensitization (Ji and Woolf 2001; Karim et al. 2001). Although several studies have addressed the modulation of pain behaviors by these kinases, little is known about the phosphorylation targets that underlie nociceptive central sensitization (Ji and Woolf 2001). Increases in neuronal excitability, such as those associated with central sensitization, frequently result from phosphorylation-dependent modulation of ion channels. Voltage-gated potassium (K+) channels are critical determinants of neuronal excitability in the central nervous system (CNS). Thus changes in K+ channel function are likely to contribute to the increased excitability in central sensitization, yet to date the role of these channels in dorsal horn neurons has not been studied in detail.
Transient potassium currents (A-type currents, IA) have been described in neurons from many regions of the CNS (Hoffman and Johnston 1998; Song et al. 1998; Wolff et al. 1998). These currents are often defined by their sensitivity to millimolar concentrations of 4-aminopyridine (4-AP), and their rapidly activating and fast inactivating characteristics. A-type channels are prominently expressed in dorsal horn neurons (Grudt and Perl 2002; Wolff et al. 1998), where their activation may reduce the slope of the rising phase of synaptic potentials (Yoshimura and Jessell 1989b). Finally, A-type potassium channels in other regions of the CNS are modulated by PKC, PKA, and ERKs (Adams et al. 2000; Anderson et al. 2000; Hoffman and Johnston 1998; Watanabe et al. 2002; Yang et al. 2001; Yuan et al. 2002). However, little is known about the modulation of potassium channel function in dorsal horn neurons by these kinases. In the present study, we tested the effects of PKC, PKA, and ERKs on A-type K+ currents in mouse superficial dorsal horn neurons to determine whether modulation of A currents by these kinases could be involved in central sensitization.
|
METHODS |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
Primary cultures of spinal cord dorsal horn neurons were prepared from 5- to 10-day-old ICR mice using a procedure modified from a previously described method (Hugel and Schlichter 2000). Briefly, after decapitation a laminectomy was performed and the spinal cord was carefully removed. Spinal cord superficial dorsal horn was dissected with a surgical blade cut in approximately lamina III. This results in cultures composed primarily of neurons from laminae I–II, although the presence of lamina III neurons cannot be entirely ruled out. The superficial dorsal horn strips were then cut into 2- to 3-mm fragments, which were incubated for 45 min at 37°C in Hanks' balanced salt solution (HBSS; Invitrogen Life Technologies, Carlsbad, CA) (in mM: 137 NaCl, 5.4 KCl, 0.4 KH2PO4, 1 CaCl2, 0.5 MgCl2, 0.4 MgSO4, 4.2 NaHCO3, 0.3 Na2HPO4, 5.6 glucose) containing papain (15 U/ml; Worthington Biochemical, Lakewood, NJ), rinsed 3 times with HBSS, and placed in culture medium containing Neurobasal (Invitrogen Life Technologies), fetal calf serum (5%, Invitrogen Life Technologies), heat-inactivated horse serum (5%, Invitrogen Life Technologies), penicillin (100 U/ml, Invitrogen Life Technologies) and streptomycin (100 µg/ml), L-glutamax-1 (2 mM, Invitrogen Life Technologies), B-27 (1%, Invitrogen Life Technologies), and glucose (20 mM, Sigma-Aldrich, St. Louis, MO). The fragments were mechanically dissociated by gently triturating with a fire-polished Pasteur pipette. The resulting cell suspension was plated onto 12-mm poly-D-lysine– and collagen-coated coverslips, and cultured for 1 to 2 days in humidified air with 5% CO2 at 37°C.
Electrophysiological recording
Whole cell recordings were made by standard procedures at room temperature with an AXOPATCH 200B amplifier and CLAMPEX 8.0 software (Axon Instruments, Union City, CA). Electrodes were pulled from filamented borosilicate glass and fire-polished. Pipette resistances were 3–6 M
. Most neurons had series resistances around 6–10 M (range, 5–18 M), which were compensated 
65%. Input resistance was 1.00 ± 0.05 G (n = 45). Most neurons had leak currents <100 pA (at –80 mV), which were not subtracted on-line. The bath solution (HBSS) contained 500 nM TTX and 2.5 mM CoCl2 to block voltage-gated Na+ currents, Ca2+ currents, and Ca2+-activated K+ currents. The electrode solution contained (in mM): 140 KCl, 1 MgCl2, 0.5 CaCl2, 5 EGTA, 10 HEPES, 3 Na2ATP, 0.3 Na2GTP, pH adjusted to 7.4 with KOH. The membrane voltage was held at –80 mV and transient potassium currents (IA) were isolated by a two-step voltage protocol. To determine the voltage dependency of activation, voltage steps of 500 ms were applied at 5-s intervals in +10-mV increments to a maximum of +70 mV. To determine the voltage dependency of inactivation, conditioning prepulses ranging from –100 to +30 mV were applied at 5-s intervals in +10-mV increments for 150 ms followed by a voltage step to +40 mV for 500 ms.
Drug application
Phorbol 12-myristate 13-acetate (PMA) and PD98059 were purchased from Sigma-Aldrich (St. Louis, MO) and dissolved in DMSO as stock solutions. 4
-PMA, forskolin (FSK), IBMX, PKI(6–22) amide, KT5720, U-0126, GF 109203X, and Ro31-8220 were obtained from Biomol (Plymouth Meeting, PA); 1,9-dideoxyforskolin from CN Biosciences (San Diego, CA); U0124 from Calbiochem (La Jolla, CA), all of which were prepared as concentrated stock solutions in DMSO. All of these were diluted to final concentrations in HBSS. Coverslips were placed in a small laminar flow perfusion chamber and continuously perfused at approximately 2–3 ml/min.
Data analysis
Off-line evaluation was done using clampfit 8.0 software (Axon Instruments) and Origin (Microcal Software, Northampton, MA). Data are expressed as original traces or as means ± SEM. The voltage dependency of activation and inactivation of IA was fitted with the Boltzmann function. For activation, peak currents were converted to conductances (G) by the formula G = I/(Vm – Vrev), where Vm is the membrane voltage of depolarization pulses and Vrev is the calculated potassium reversal potential (–84 mV). The function G/Gmax = 1/(1 + exp[(V1/2 – V)/k]) was used, where Gmax is the maximal conductance obtained with a depolarizing pulse to +70 mV, V1/2 is the half-maximal voltage, and k is the slope factor. For inactivation, I/Imax = 1/(1 + exp[(V1/2 – V)/k]) was used, where Imax is the maximal current obtained with a –100 mV prepulse. Treatment effects were statistically analyzed by one-way ANOVA followed by Bonferroni's multiple comparison test. Paired or two-sample t-test was used when comparisons were restricted to two means. Error probabilities of P < 0.05 were considered statistically significant.
|
RESULTS |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
|
PKC decreases A-type potassium currents in dorsal horn neurons
As mentioned above, PKC has been implicated in central sensitization (Coderre 1992) and also shown to be a modulator of the A current in hippocampal dendrites (Hoffman and Johnston 1998; Yuan et al. 2002). To ascertain the effects of PKC activation on A currents in dorsal horn neurons, we bath-applied the PKC activator PMA (2.5–10 µM) and measured the changes in the peak amplitude of A currents. We found that 5 µM PMA was the lowest concentration necessary to elicit the maximal effect on IA, decreasing the peak current (measured at a step of depolarization to +40 mV from –80 mV holding potential) by 22.1 ± 2.0% (Fig. 2, A–C). The decay rate of A-type current was best fitted with double exponential (at +40 mV). Neither the fast nor the slow time constant was changed after PMA treatment (Table 2). The control analog for PMA, 4-PMA (5 µM), had no effect on A-type currents (Fig. 2, A and B). Steady-state inactivation and activation curves generated from these experiments show a shift of the activation curve to the right 7.3 mV and a shift of the inactivation curve to the left 12.4 mV in response to 5 µM PMA (Fig. 2D, Table 1). The slope factors of both the activation and the inactivation curves were significantly changed (Table 1). These data indicate that PMA modulates A-type potassium channels, at least in part, by reducing both the total current produced by the channels and the percentage of channels available for activation.
|
|
|
|
|
|
To confirm that the effect of PMA on IA was a direct result of PKC activation, we tested the effects of the PKC inhibitor GF 109203X (GF) (Toullec et al. 1991) on A currents. Bath-application of a range of concentrations (0.25–2 µM) of GF increased IA in a dose-dependent manner (Fig. 3, A and B). The decay kinetics were changed by higher concentrations of GF. The slow time constant was decreased from 120 ± 9 to 76 ± 4 ms after 1 µM GF treatment without changing the fast time constant (Table 2). A significant shift of the activation curve to the left 8.8 mV and of the inactivation curve to the right 7.5 mV (Fig. 3C, Table 1) resulted from application of 1 µM GF. The slope factor of the inactivation curve was also significantly changed (Table 1). Application of 5 µM GF produced a smaller enhancement of the A current (15.5 ± 2.3, n = 4, P < 0.05 compared with 2 µM GF 49.3 ± 5.0), likely as a result of nonspecific effects. In contrast to the effect of PMA on A currents, GF enhanced the activation of A-type channels, increasing A currents.
To test whether PKC inhibitors block the inhibition of IA by PMA, we first applied GF and subsequently tested PMA's ability to reverse the GF-mediated enhancement of IA. Application of 1 µM GF induced a time-dependent increase in A currents that reached a plateau at 37.1 ± 7.0% above the basal current after 5 min. Consistent with our hypothesis that the inhibition of IA by PMA was attributed to PKC activation, PMA did not significantly decrease IA in the presence of GF (Fig. 4, B–D). Another selective PKC inhibitor, Ro31-8220 (1 µM) (McKenna and Hanson 1993; Shen and Glazer 1998), also enhanced A currents (by 18.5 ± 4.6%), decreased the slow time constant (Table 2), and prevented PMA's effect on the A current (Fig. 4D).
|
PKA activation suppresses A-type currents
Activation of PKA is also implicated in central sensitization (Malmberg et al. 1997; Sluka 2002). To determine whether PKA modulates A-type currents in dorsal horn neurons, we examined the effects of forskolin (FSK), an adenylyl cyclase activator, on these channels. The A-current peak amplitude was reduced by 18.3 ± 2.9% after application of 50 µM FSK with 50 µM IBMX (Fig. 5, A and D). The decay time constants (both of fast and slow) were reduced after FSK treatment (Table 2). FSK caused a slight but significant rightward shift in the activation curve (V1/2, –3.8 ± 1.0 and 0.3 ± 1.3 mV for control and FSK, respectively, n = 6, P < 0.05; Fig. 5C, Table 1). We also found the inactivation curve to be shifted toward the left –9.4 mV (Fig. 5C, Table 1). Similarly, 1 mM 8Br-cAMP, a membrane-permeable analog of cAMP, significantly reduced the peak amplitude of the A current by 14.6 ± 1.6% (n = 5, P < 0.05; Fig. 5, A and D), but did not change its kinetics (Table 2). The negative control for FSK, 1,9-dideoxyforskolin (50 µM) did not decrease the A current (Fig. 5, A and D). KT5720 (1 µM), a specific inhibitor of PKA (Hidaka et al. 1984), had no significant effect on the A current when applied alone, but blocked the inhibition of IA by FSK (Fig. 5D). When 50 µM PKI(6–22) amide, another specific PKA inhibitor (Kemp et al. 1988), was added to the patch pipette, bath-application of FSK had no effect on A currents (Fig. 5, A and D), but still significantly decreased both the fast and slow time constants. These results indicate that inhibition of peak amplitude of A current by FSK is attributable to PKA activation, and the change in kinetics is likely a nonspecific effect on the channel.
Inhibition of ERK signaling enhances A-type currents
We previously demonstrated that ERK activation is necessary for dorsal horn plasticity in inflammatory pain (Karim et al. 2001). Because there are no direct activators or inhibitors of ERK, to test whether ERK activation modulates the A-type currents in dorsal horn, we applied PD98059 and U0126 (inhibitors of mitogen-activated/ERK kinase (MEK), the upstream activator of ERKs) to the neurons. PD98059 (20 µM) significantly increased A currents by 21.7 ± 3.8% without changing their kinetics (Table 2), and shifted the activation curve toward the left –6.3 mV (Fig. 6C, Table 1) while shifting the inactivation curve toward the right 7.2 mV (Fig. 6C, Table 1). Similarly, 0.5 µM U0126 increased the A current by 20.7 ± 4.7% (Fig. 6, A and B). Bath-application of U0124 (0.5 µM), a negative control analog of U0126, had no significant effect on IA (Fig. 6, A and B). These results suggested that there is basal ERK activity in dorsal horn neurons that tonically inhibits IA in these neurons.
ERK inhibitors block PKC and PKA inhibition of A-type currents
The results discussed above show that the effect of ERK is similar to that of PKA and PKC, acting to inhibit the A current. This led us to question the pathway by which the kinases were acting. Both PKA and PKC activation can stimulate ERK (Roberson et al. 1999; Yuan et al. 2002). Do PKA and PKC act through ERK, or do these kinases directly modulate A-type potassium channels? To determine whether the PKC- and PKA-induced suppression of the A current depends on activation of the ERK pathway, we examined the effects of the MEK inhibitor PD98059 on the PMA- and FSK-mediated decrease in A currents. As shown in Fig. 7, neurons were exposed to 20 µM PD98059 for 5 min to inhibit activation of ERK. This led to a gradual increase in IA that reached a plateau (Fig. 7, A and B). Neither PMA nor FSK reduced A currents in the presence of PD98059, indicating that blocking ERK activation also blocks the effects of PKC and PKA on A currents (Fig. 7C).
|
Finally, we tested the effect of the PKC inhibitor GF on the PD98059-mediated increase in A currents. As shown in Fig. 8, 2 µM GF elicited an increase in the A current of 43.2 ± 3.9%. PD98059 (20 µM) did not produce a further increase in IA in the presence of GF. These results are consistent with the hypothesis that PKC is acting upstream of ERK to modulate IA.
|
|
DISCUSSION |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
; Johnston et al. 1999). Furthermore, PKA, PKC, and ERK are localized in dendrites along with the A-type potassium channels, and inhibition and activation of these kinases modulates A currents (Yuan et al. 2002). ERK is known to phosphorylate Kv4.2, an A-type potassium channel subunit (Adams et al. 2000), and was shown to modulate the effects of GDNF on transient potassium channel currents (Yang et al. 2001). Because PKC, PKA, and ERK play important roles both in nociception and modulation of A-type potassium channels, we sought to test whether these kinases modulate A-type potassium channels in spinal cord dorsal horn neurons.
Our patch-clamp data demonstrate that outward currents recorded in our conditions are consistent with those of A-type channels in rat spinal dorsal horn neurons in slice (Wolff et al. 1998; Yoshimura and Jessell 1989a). The A-type K+ channel subunit, Kv4.2, has been shown to localize to some central neurons (Alonso and Widmer 1997; Sheng et al. 1992), and Kv4.2 is a substrate for PKA- and ERK-mediated phosphorylation (Adams et al. 2000; Anderson et al. 2000). Our data show that A currents in dorsal horn neurons have many pharmacological and biophysical properties, such as activation, inactivation, and recovery from inactivation, that are similar to those of Kv4 subunits (Bahring et al. 2001; Tseng et al. 1996), indicating that Kv4 channels may be expressed in dorsal horn neurons. Further studies using anatomical and genetic approaches are needed to identify the exact Kv subunits that underlie IA in dorsal horn neurons, and further which subunits mediate the kinase-dependent regulation of IA demonstrated here.
This study provides strong support for the hypothesis that PKC, PKA, and ERK are involved in modulation of A-type currents in spinal cord superficial dorsal horn neurons. Activation of PKC had significant effects on IA, reducing its amplitude without changing current kinetics, and shifting the activation and the inactivation curves. In contrast, inhibition of PKC dramatically and dose-dependently increased A-type currents and shifted the activation and inactivation curves in the opposite direction. Interestingly, PKC inhibitors increased the rate of current decay for the slow component but not the fast component. This change in current decay kinetics may be a nonspecific effect of the PKC inhibitors because, as mentioned above, PKC activators had no effect on current decay kinetics.
Activation of PKA by forskolin also decreased A currents and shifted the activation and inactivation curves. This effect was mimicked by the PKA activator 8-Br-cAMP and blocked by the specific peptide inhibitor of PKA, PKI(6–22) amide. Forskolin has been shown to have nonspecific effects on some types of potassium channels (Herness et al. 1997). We observed this nonspecific effect as an increasing current decay rate of both the fast and slow components, which was not blocked by PKI(6–22) amide. MEK inhibitors also resulted in an increase in A-type currents and shifted both the activation and inactivation curves without changing channel kinetics, whereas a negative control analog had no effect on A-type currents.
Our data suggest that PKC, PKA, and ERK modulate A-current activation and inactivation. It is likely that these gating changes are at least in part responsible for the inhibition of peak currents we observed. It is interesting to note that activators of PKC and PKA appear to induce nonparallel shifts in the activation and inactivation curves. This suggests that there may be heterogeneity in the channels that are mediating the A currents in these neurons, and that these activators may have differential or voltage-dependent effects on these channels. Future studies will endeavor to identify the specific Kv subunits that mediate the A currents in dorsal horn neurons.
Experiments in rat primary sensory neurons separated the PKC, PKA, and ERK pathways to hyperalgesia by showing that inhibition of the ERK pathway did not affect hyperalgesia produced by PKA or PKC activation and also that PKA and PKC inhibitors were unable to block hyperalgesia mediated through the ERK pathway (Aley et al. 2001). This was not necessarily the case in dorsal horn neurons. We addressed the issue by combining inhibitors of ERK with activators of PKC or PKA, and our data demonstrate that ERK inhibition blocks the effects of both PKC and PKA on A-type currents. This finding is consistent with the idea that ERK activation and modulation of IA are downstream of both PKA and PKC, as shown in the model depicted in Fig. 8C. Alternatively, it is also possible that PKA and PKC modulation of KA requires prior phosphorylation by ERK to achieve this modulation. Our data cannot rule out either model. The potential mechanism of activation of ERK by PKA and PKC in dorsal horn neurons is unknown. Recent studies suggest that PKC might act on Raf, and PKA on B-Raf through Rap1 to activate ERK (Gutkind 2000). Thus the PKA- and PKC-mediated signaling pathways converge onto ERKs, which we suggest phosphorylate A-type K+ channel subunits in dorsal horn neurons.
Prolonged activation of nociceptive primary afferents by noxious stimuli can increase the excitability of nociceptive neurons in the dorsal horn (Ji and Woolf 2001). This central sensitization is the result of the activation of several kinase cascades, some of which have been linked in this study to modulation of the A-type potassium current by ERK. Studies presented in the accompanying report show that the changes in KA reported here are paralleled by the predicted changes in action firing properties, leading to a dramatic increase in excitability of dorsal horn neurons (Hu and Gereau 2003). We propose that ERK modulation of A-type potassium channels may play an important role in establishing central sensitization associated with injury and disease.
The results obtained here from cultures of superficial dorsal horn neurons leave some open questions. For example, are similar changes seen in identified laminae I–II neurons in acute slices? Although we isolated only the most superficial portion of the dorsal horn, our cultures may contain some cells from deeper laminae. Will similar neuromodulatory effects be seen in cells from more mature animals? The roles of PKC, PKA, and ERK in modulating pain perception were established in adult animals, whereas the cultures used here were prepared from very young animals. Are A-type currents seen mostly in glutamatergic cells or do they and their modulation also have an impact on the physiological properties of inhibitory interneurons? All of these questions are difficult to answer using cell culture systems. These studies must be extended to recordings from visually identified and characterized neurons in acute slices from more mature animals. Such efforts will help to answer these questions and provide a more direct link between the types of neuromodulation we have identified here and the modulation of nociception identified at the behavioral level.
|
DISCLOSURES |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
|
ACKNOWLEDGMENTS |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
|
FOOTNOTES |
|---|
Address for reprint requests: Robert W. Gereau IV, Division of Neuroscience, Baylor College of Medicine, Houston, TX 77030 (E-mail: rgereau{at}bcm.tmc.edu).
|
REFERENCES |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONDISCLOSURESACKNOWLEDGMENTSREFERENCES |
|---|
Aley KO, Martin A, McMahon T, Mok J, Levine JD, and Messing RO. Nociceptor sensitization by extracellular signal-regulated kinases. J Neurosci 21: 6933–6939, 2001.
Alonso G and Widmer H. Clustering of KV4.2 potassium channels in postsynaptic membrane of rat supraoptic neurons: an ultrastructural study. Neuroscience 77: 617–621, 1997.[Web of Science][Medline]
Anderson AE, Adams JP, Qian Y, Cook RG, Pfaffinger PJ, and Sweatt JD. Kv4.2 phosphorylation by cyclic AMP-dependent protein kinase. J Biol Chem 275: 5337–5346, 2000.
Bahring R, Boland LM, Varghese A, Gebauer M, and Pongs O. Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channels. J Physiol 535: 65–81, 2001.
Coderre TJ. Contribution of protein kinase C to central sensitization and persistent pain following tissue injury. Neurosci Lett 140: 181–184, 1992.[Web of Science][Medline]
Eide PK. Pathophysiological mechanisms of central neuropathic pain after spinal cord injury. Spinal Cord 36: 601–612, 1998.[Web of Science][Medline]
Grudt TJ and Perl ER. Correlations between neuronal morphology and electrophysiological features in the rodent superficial dorsal horn. J Physiol 540: 189–207, 2002.
Gutkind JS. Regulation of mitogen-activated protein kinase signaling networks by G protein-coupled receptors. Sci STKE 2000: RE1, 2000.
Hidaka H, Inagaki M, Kawamoto S, and Sasaki Y. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry 23: 5036–5041, 1984.[Medline]
Hoffman DA and Johnston D. Downregulation of transient K+ channels in dendrites of hippocampal CA1 pyramidal neurons by activation of PKA and PKC. J Neurosci 18: 3521–3528, 1998.
Hu HJ and Gereau RW. ERK integrates PKA and PKC signaling in superficial dorsal horn neurons. II. Modulation of neuronal excitability. J Neurophysiol 90: 1680–1688, 2003.
Hugel S and Schlichter R. Presynaptic P2X receptors facilitate inhibitory GABAergic transmission between cultured rat spinal cord dorsal horn neurons. J Neurosci 20: 2121–2130, 2000.
Ji RR and Woolf CJ. Neuronal plasticity and signal transduction in nociceptive neurons: implications for the initiation and maintenance of pathological pain. Neurobiol Dis 8: 1–10, 2001.[Web of Science][Medline]
Johnston D, Hoffman DA, Colbert CM, and Magee JC. Regulation of back-propagating action potentials in hippocampal neurons. Curr Opin Neurobiol 9: 288–292, 1999.[Web of Science][Medline]
Karim F, Wang C-C, and Gereau RW. Metabotropic glutamate receptor subtypes 1 and 5 are activators of extracellular signal-regulated kinase signaling required for inflammatory pain in mice. J Neurosci 21: 3771–3779, 2001.
Kemp BE, Cheng HC, and Walsh DA. Peptide inhibitors of cAMP-dependent protein kinase. Methods Enzymol 159: 173–183, 1988.[Web of Science][Medline]
Malmberg AB, Brandon EP, Idzerda RL, Liu H, McKnight GS, and Basbaum AI. Diminished inflammation and nociceptive pain with preservation of neuropathic pain in mice with a targeted mutation of the type I regulatory subunit of cAMP-dependent protein kinase. J Neurosci 17: 7462–7470, 1997.
McKenna JP and Hanson PJ. Inhibition by Ro 31-8220 of acid secretory activity induced by carbachol indicates a stimulatory role for protein kinase C in the action of muscarinic agonists on isolated rat parietal cells. Biochem Pharmacol 46: 583–588, 1993.[Web of Science][Medline]
Roberson ED, English JD, Adams JP, Selcher JC, Kondratick C, and Sweatt JD. The mitogen-activated protein kinase cascade couples PKA and PKC to cAMP response element binding protein phosphorylation in area CA1 of hippocampus. J Neurosci 19: 4337–4348, 1999.
Shen L and Glazer RI. Induction of apoptosis in glioblastoma cells by inhibition of protein kinase C and its association with the rapid accumulation of p53 and induction of the insulin-like growth factor-1-binding protein-3. Biochem Pharmacol 55: 1711–1719, 1998.[Web of Science][Medline]
Sheng M, Tsaur ML, Jan YN, and Jan LY. Subcellular segregation of two A-type K+ channel proteins in rat central neurons. Neuron 9: 271–284, 1992.[Web of Science][Medline]
Sluka KA. Stimulation of deep somatic tissue with capsaicin produces long-lasting mechanical allodynia and heat hypoalgesia that depends on early activation of the cAMP pathway. J Neurosci 22: 5687–5693, 2002.
Song WJ, Tkatch T, Baranauskas G, Ichinohe N, Kitai ST, and Surmeier DJ. Somatodendritic depolarization-activated potassium currents in rat neostriatal cholinergic interneurons are predominantly of the A type and attributable to coexpression of Kv4.2 and Kv4.1 subunits. J Neurosci 18: 3124–3137, 1998.
Toullec D, Pianetti P, Coste H, Bellevergue P, Grand-Perret T, Ajakane M, Baudet V, Boissin P, Boursier E, Loriolle F, Duhamel L, Charon D, and Kirilovsky J. The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem 266: 15771–15781, 1991.
Tseng GN, Jiang M, and Yao JA. Reverse use dependence of Kv4.2 blockade by 4-aminopyridine. J Pharmacol Exp Ther 279: 865–876, 1996.
Watanabe S, Hoffman DA, Migliore M, and Johnston D. Dendritic K+ channels contribute to spike-timing dependent long-term potentiation in hippocampal pyramidal neurons. Proc Natl Acad Sci USA 99: 8366–8371, 2002.
Wolff M, Vogel W, and Safronov BV. Uneven distribution of K+ channels in soma, axon and dendrites of rat spinal neurones: functional role of the soma in generation of action potentials. J Physiol 509: 767–776, 1998.
Yang F, Feng L, Zheng F, Johnson SW, Du J, Shen L, Wu CP, and Lu B. GDNF acutely modulates excitability and A-type K(+) channels in midbrain dopaminergic neurons. Nat Neurosci 4: 1071–1078, 2001.[Web of Science][Medline]
Yoshimura M and Jessell TM. Membrane properties of rat substantia gelatinosa neurons in vitro. J Neurophysiol 62: 109–118, 1989a.
Yoshimura M and Jessell TM. Primary afferent-evoked synaptic responses and slow potential generation in rat substantia gelatinosa neurons in vitro. J Neurophysiol 62: 96–108, 1989b.
Yuan LL, Adams JP, Swank M, Sweatt JD, and Johnston D. Protein kinase modulation of dendritic K+ channels in hippocampus involves a mitogen-activated protein kinase pathway. J Neurosci 22: 4860–4868, 2002.
This article has been cited by other articles:
|
|
 |
|
  J. Sandkuhler Models and Mechanisms of Hyperalgesia and Allodynia Physiol Rev, April 1, 2009; 89(2): 707 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Liang, H. Wang, H. Chen, Y. Cui, L. Gu, J. Chai, and K. Wang Structural Insights into KChIP4a Modulation of Kv4.3 Inactivation J. Biol. Chem., February 20, 2009; 284(8): 4960 - 4967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Y. Teos, A. Zhao, Z. Alvin, G. G. Laurence, C. Li, and G. E. Haddad Basal and IGF-I-dependent regulation of potassium channels by MAP kinases and PI3-kinase during eccentric cardiac hypertrophy Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H1834 - H1845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. I. Cohen-Matsliah, Y. Seroussi, K. Rosenblum, and E. Barkai Persistent ERK activation maintains learning-induced long-lasting modulation of synaptic connectivity Learn. Mem., October 2, 2008; 15(10): 756 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kohno, H. Wang, F. Amaya, G. J. Brenner, J.-K. Cheng, R.-R. Ji, and C. J. Woolf Bradykinin Enhances AMPA and NMDA Receptor Activity in Spinal Cord Dorsal Horn Neurons by Activating Multiple Kinases to Produce Pain Hypersensitivity J. Neurosci., April 23, 2008; 28(17): 4533 - 4540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Wang and G. I. Hatton Interaction of Extracellular Signal-Regulated Protein Kinase 1/2 with Actin Cytoskeleton in Supraoptic Oxytocin Neurons and Astrocytes: Role in Burst Firing J. Neurosci., December 12, 2007; 27(50): 13822 - 13834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Hu, B. J. Alter, Y. Carrasquillo, C.-S. Qiu, and R. W. Gereau IV Metabotropic Glutamate Receptor 5 Modulates Nociceptive Plasticity via Extracellular Signal-Regulated Kinase Kv4.2 Signaling in Spinal Cord Dorsal Horn Neurons J. Neurosci., November 28, 2007; 27(48): 13181 - 13191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Cohen-Matsliah, I. Brosh, K. Rosenblum, and E. Barkai A Novel Role for Extracellular Signal-Regulated Kinase in Maintaining Long-Term Memory-Relevant Excitability Changes J. Neurosci., November 14, 2007; 27(46): 12584 - 12589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. A. Santos, S. Rebelo, V. A. Derkach, and B. V. Safronov Excitatory interneurons dominate sensory processing in the spinal substantia gelatinosa of rat J. Physiol., May 15, 2007; 581(1): 241 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Carrasquillo and R. W. Gereau IV Activation of the Extracellular Signal-Regulated Kinase in the Amygdala Modulates Pain Perception J. Neurosci., February 14, 2007; 27(7): 1543 - 1551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ruiz-Gomez, B. Mellstrom, D. Tornero, E. Morato, M. Savignac, H. Holguin, K. Aurrekoetxea, P. Gonzalez, C. Gonzalez-Garcia, V. Cena, et al. G Protein-coupled Receptor Kinase 2-mediated Phosphorylation of Downstream Regulatory Element Antagonist Modulator Regulates Membrane Trafficking of Kv4.2 Potassium Channel J. Biol. Chem., January 12, 2007; 282(2): 1205 - 1215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Sun, B. Yang, D. F. Donnelly, C. Ma, and R. H. LaMotte MCP-1 Enhances Excitability of Nociceptive Neurons in Chronically Compressed Dorsal Root Ganglia J Neurophysiol, November 1, 2006; 96(5): 2189 - 2199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Schrader, S. G. Birnbaum, B. M. Nadin, Y. Ren, D. Bui, A. E. Anderson, and J. D. Sweatt ERK/MAPK regulates the Kv4.2 potassium channel by direct phosphorylation of the pore-forming subunit Am J Physiol Cell Physiol, March 1, 2006; 290(3): C852 - C861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Wei, K. I. Vadakkan, H. Toyoda, L.-J. Wu, M.-G. Zhao, H. Xu, F. W.F. Shum, Y. H. Jia, and M. Zhuo Calcium Calmodulin-Stimulated Adenylyl Cyclases Contribute to Activation of Extracellular Signal-Regulated Kinase in Spinal Dorsal Horn Neurons in Adult Rats and Mice J. Neurosci., January 18, 2006; 26(3): 851 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-L. Yuan, X. Chen, K. Kunjilwar, P. Pfaffinger, and D. Johnston Acceleration of K+ channel inactivation by MEK inhibitor U0126 Am J Physiol Cell Physiol, January 1, 2006; 290(1): C165 - C171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Michlig, A. Mercier, A. Doucet, L. Schild, J.-D. Horisberger, B. C. Rossier, and D. Firsov ERK1/2 Controls Na,K-ATPase Activity and Transepithelial Sodium Transport in the Principal Cell of the Cortical Collecting Duct of the Mouse Kidney J. Biol. Chem., December 3, 2004; 279(49): 51002 - 51012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.-Q. Sun, Y.-J. Tu, N. B. Lawand, J.-Y. Yan, Q. Lin, and W. D. Willis Calcitonin Gene-Related Peptide Receptor Activation Produces PKA- and PKC-Dependent Mechanical Hyperalgesia and Central Sensitization J Neurophysiol, November 1, 2004; 92(5): 2859 - 2866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-R. Ji and G. Strichartz Cell Signaling and the Genesis of Neuropathic Pain Sci. Signal., September 28, 2004; 2004(252): re14 - re14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kawasaki, T. Kohno, Z.-Y. Zhuang, G. J. Brenner, H. Wang, C. Van Der Meer, K. Befort, C. J. Woolf, and R.-R. Ji Ionotropic and Metabotropic Receptors, Protein Kinase A, Protein Kinase C, and Src Contribute to C-Fiber-Induced ERK Activation and cAMP Response Element-Binding Protein Phosphorylation in Dorsal Horn Neurons, Leading to Central Sensitization J. Neurosci., September 22, 2004; 24(38): 8310 - 8321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Hu and R. W. Gereau IV ERK Integrates PKA and PKC Signaling in Superficial Dorsal Horn Neurons. II. Modulation of Neuronal Excitability J Neurophysiol, September 1, 2003; 90(3): 1680 - 1688. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
