Topography and Response Timing of Intact Cerebellum Stained With Absorbance Voltage-Sensitive Dye
J Neurophysiol Brown and Ariel
101: 474
Supplemental Figures and Video
19 video files in MPG format; 4 images in PDF format.
Files in this Data Supplement:
Supplement to Figure 4C -
Photograph of stained turtle cerebellum, ventricular side up, transilluminated by the 150 W tungsten-halogen bulb through the microscope condenser. The magnified view of Figure 4C shows the placement of the bipolar stimulating electrode on the ventricular surface of rostrocaudal fiber bundles (white matter). The edge of the cover glass is visible on the left side, resulting in a displaced unfocused second image of the electrode. Unlike the true color of figure 4C photographed in ambient illumination, the color of this transilluminated tissue is distorted.
Supplement to Figure 5A -
Photograph of cerebellum, showing magnified view of top photograph of Figure 5A. Refer to legend of Supp. Fig. 4C. Note that this cerebellum is larger because it was removed from a 30 cm turtle.
Supplement to Figure 5B -
Photograph of cerebellum. Refer to legend of Supp. Fig. 5A, except this is a magnified view of top photograph of Figure 5B.
Supplement to Figure 8D (colored traces) -
Spatial display of traces for diodes that imaged the cerebellum, pial side up, superimposed on a monochrome image of the tissue. Blue lines represent positions of depolarizing responses; orange lines represent positions of hyperpolarizing responses. The transverse place of the tissue was not placed horizontal in the chamber so, unlike Figure 8D, this display is not corrected for that misalignment.
Video - Supplement to Figure 2
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Pseudocolor movie superimposed on photograph of stained turtle brain preparation. A red dot flashes at the onset of the inferior olive microstimulation.
Video - Supplement to Figure 3A
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Pseudocolor movie superimposed on photograph of turtle cerebellum, ventricular side up. A red dot flashes in the upper left at the onset of the inferior olive microstimulation. A black line in the color bar on the right indicates the pseudocolor threshold.
Video - Supplement to Figure 5A (top panel)
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Pseudocolor movie superimposed on photograph of turtle cerebellum, ventricular side up. White matter microstimulation occurs when thee red dot flashes in the upper left corner. A black line in the color bar on the right indicates the pseudocolor threshold.
Video - Supplement to Figure 5B (top panel)
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Pseudocolor movie. Refer to legend of Supp. Fig. 5A (top), except the cerebellum is pial side up and the stimulation is 700 µm below the surface.
Video - Supplement to Figure 5B (middle panel)
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Pseudocolor movie. Refer to legend of Supp. Fig. 5A (top), except the cerebellum is pial side up and the stimulation is 700 µm below the surface.
Video - Supplement to Figure 5B (bottom panel)
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Pseudocolor movie. Refer to legend of Supp. Fig. 5A (top), except the cerebellum is pial side up and the stimulation is 700 µm below the surface.
Video - Supplement to Figure 5C (middle panel)
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Pseudocolor movie. Refer to legend of Supp. Fig. 5A (top), except the stimulation intensity is 15 µA, 400 µm below the surface. The movie was generated using a pseudocolor threshold half that of Figure 5A,B. Note the black line in the color bar on the right indicates the lower threshold.
Video - Supplement to Figure 6 (top row)
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Pseudocolor movie superimposed on photograph of the cerebellum, ventricular side up. A red dot flashes in the upper left at the onset of the inferior olive microstimulation.
Video - Supplement to Figure 6 (middle row)
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Pseudocolor movie superimposed on photograph of the cerebellum, ventricular side up. A red dot flashes in the upper left at the onset of the white matter microstimulation.
Video - Supplement to Figure 6 (bottom row)
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Pseudocolor movie superimposed on photograph of the cerebellum, ventricular side up. A red dot flashes in the upper left at the onset of the molecular layer microstimulation.
Video - Supplement to Figure 8A
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Pseudocolor movie superimposed on photograph of the cerebellum, pial side up. A red dot flashes at the onset of microstimulation within the cerebellum.
Video - Supplement to Figure 8B
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Pseudocolor movie superimposed on photograph of the cerebellum, pial side up. A red dot flashes at the onset of the molecular layer microstimulation.
Video - Supplement to Figure 8B filtered
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Pseudocolor movie. Refer to legend of Supp. Fig. 5A (top), except the movie was generated using a high pass filter to demonstrate the bidirectional motion of the response.
Video - Supplement to Figure 8C (left panel)
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Pseudocolor movie superimposed on photograph of the cerebellum, pial side up. A red dot flashes in the upper left at the onset of the molecular layer microstimulation.
Video - Supplement to Figure 8C (right panel)
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Pseudocolor movie superimposed on photograph of the cerebellum, pial side up. A red dot flashes in the upper left at the onset of the inferior olive microstimulation.
Video - Supplement to Figure 8D
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Pseudocolor movie superimposed on photograph of the cerebellum, pial side up. A red dot flashes in the upper left at the onset of the molecular layer microstimulation
Video - Supplement to Figure 8D (no threshold)
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Pseudocolor movie. Refer to legend of Supp. Fig. 8D, except the movie was generated without a pseudocolor threshold, thereby revealing depolarizing and hyperpolarizing responses.