Rapid and Sensitive Mapping of Long-Range Connections In Vitro Using Flavoprotein Autofluorescence Imaging Combined With Laser Photostimulation
J Neurophysiol Llano et al.
101: 3325
Supplemental Figures and Videos
2 video files in AVI format, plus 2 figures in PDF format.
Video 1
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Real-time illustration of laser-mapping of projections from VPM-TRN, as shown in the still images in Figure 11. Amplitude of response is expressed as change in pixel intensity averaged over five frames, compared to previous five frames (Δf/f slope).
Video 2
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Real-time illustration of the spatiotemporal pattern of activation in the S1BF after picospritzing 2mM L-glutamic acid (in ACSF) into the VPM. Data are the same as in Figure 3. Shown is a series of Δf/f images. Stimulus parameters were 20 Hz pulse train, 8 msec pulse width, 1 psi pulse amplitude, total train duration of 1 second. Acquisition at 4 frames per second.