| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Vollum Institute, Oregon Health and Science University, Portland, Oregon
Submitted 28 July 2005; accepted in final form 14 August 2005
 |
ABSTRACT |
|---|
 TOP ABSTRACT INTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
|
INTRODUCTION |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
). These actions potentials then backpropagate into the extensive lateral dendrites within the external plexiform layer (EPL). Lateral dendrites taper along their course and are the site of lateral and recurrent inhibition mediated by dendrodendritic synapses with granule cells. Action potentials in lateral dendrites are more labile than in apical dendrites and thus are particularly subject to modulation by both intrinsic and synaptic conductances (Christie and Westbrook 2003; Lowe 2002; Xiong and Chen 2002). The summed activity of the olfactory bulb is relayed to the olfactory cortex as action potential trains in mitral cell axons.
At dendrodendritic synapses in lateral dendrites, mitral cells release glutamate, whereas adjacent granule cell dendrites release GABA (Isaacson and Strowbridge 1998; Schoppa et al. 1998). Glutamate activates not only receptors on "postsynaptic" granule cells but also AMPA and NMDA autoreceptors on the dendrite adjacent to the release site (Isaacson 1999; Montague and Greer 1999; Petralia et al. 1994; Schoppa and Westbrook 2002). NMDA autoreceptors may be important for temporal patterning of action potentials (Friedman and Strowbridge 2000), but the intrinsic membrane properties of mitral cell dendrites also are important for dendritic excitability. For example, in mitral cell lateral dendrites, backpropagating action potentials can be regulated by A-type potassium channels (Christie and Westbrook 2003; Davison et al. 2004). In other areas of the brain, calcium-activated potassium channels regulate action potential interspike interval and spike frequency adaptation (Sah and Faber 2002; Vergara et al. 1998). Small-conductance calcium-activated potassium (SK) channels, responsible for slow afterhyperpolarizations in CA1 pyramidal cells (Bond et al. 2004), are also expressed in the olfactory bulb (Stocker and Pedarzani 2000).
We examined the role of SK channels in olfactory bulb using whole cell recordings. Mitral cells, but not granule cells, had apamin-sensitive potassium currents consistent with expression of SK channels. Inhibition of SK channels increased action potential firing in mitral cells and thus increased dendrodendritic inhibition. SK currents and NMDA autoreceptor currents had a similar time course, and NMDA receptor-mediated calcium influx was capable of activating SK channels.
|
METHODS |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
Olfactory bulb slices were prepared from 9- to 20-day-old C57BL6/J. Mice were deeply anesthetized with halothane and then decapitated per protocols approved by the IACUC at Oregon Health and Science University. Bulbs were rapidly removed and immersed in ice-cold oxygenated (95% O2-5% CO2) artificial cerebrospinal fluid (ACSF). This solution was the same as our standard extracellular recording solution (see following text) except that 2 mM CaCl2 and 1 mM MgCl2 were replaced with 0.5 mM CaCl2 and 4 mM MgCl2. Horizontal slices (290 µM) were cut using a vibrating blade vibrotome (VT1000S; Leica, Bannockburn, IL). Slices were incubated in an oxygenated holding chamber for 30 min at 37°C and then stored at room temperature.
Slices were visualized using infrared differential interference contrast microscopy (IR/DIC, Zeiss Axioskop) equipped with a CCD camera (XC-ST30, Sony, Japan) and 40x Zeiss water-immersion objective (0.75 N.A.). Mitral and granule cells could be discriminated easily on the basis of morphology. All experiments were performed at 31–34°C.
Voltage-clamp recordings
Whole cell patch recording from mitral and granule cells were obtained using previously described methods (Stuart et al. 1993). For all experiments, oxygenated (95% O2-5% CO2) ACSF contained (in mM) 125 NaCl, 25 NaHCO3, 1.25 NaH2PO4, 3 KCl, and 25 dextrose, pH 7.3. Depending on the experiment, as indicated, we added either 100 µM or 1 mM MgCl2 and 0.5 mM or 2 mM CaCl2. For some experiments, synaptic currents were blocked with 100 µM D,L-2-amino-5-phosphonopentanoic acid (AP5), 10 µM 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzoquinoxaline-7-sulfonamide (NBQX), 5 µM gabazine (Tocris, Ellisville, MO); sodium conductances were blocked with 0.5 µM TTX; and voltage-dependent K+ channels and BK channels were blocked with 1 mM tetraethylammonium chloride (TEA). Patch pipettes were fabricated from borosilicate glass (TW150F-6; WPI, Sarasota, FL) with resistances of 1–3 M
for mitral cells and 3–7 M for granule cells. Except where indicated, pipettes were filled with (in mM) 135 Kgluconate, 10 KCl, 1 MgCl2, 10 HEPES, 2 Na-ATP, and 0.4 Na-GTP; pH was adjusted to 7.3 with KOH. To minimize the effect of exogenous buffers on intracellular Ca2+, pipettes contained 0.5 mM EGTA. Mitral cell IPSCs were recorded using an internal solution containing (in mM) 125 KCl, 2 MgCl2, 10 HEPES, 2 Na-ATP, 0.4 Na-GTP, and 0.5 EGTA, adjusted to pH 7.3 with KOH.
Voltage-clamp currents, recorded with a Multiclamp 700A amplifier (Axon instruments, Foster City, CA), were filtered at 2 kHz (4-pole Bessell) and digitized at 10 kHz. Data were acquired using Axograph 4.9 (Axon Instruments). In all recordings membrane voltage Vm and access resistance Rs were monitored. Data acquisition was terminated when Vm was more positive than –48 mV or Rs was >15 M. The holding potential for current recordings was –55 or –70 mV as indicated for each experiment. For electrical stimulation of the glomerular layer, we used a tungsten bipolar electrode (FHC, Brunswick, ME) placed on the surface of the slice. Computer-generated stimulation pulses triggered a stimulus isolation unit (10–100 V, 0.1 ms; Digitimer, Herfordshire, UK).
Current-clamp recordings
For measurements of cell firing patterns, mitral and granule cells were held at –65 to –72 mV by steady current injection. Action potential trains were evoked with current pulses (100–400 pA, 3 s). Experiments were terminated if action potential threshold or amplitude changed during recordings.
Data analysis and statistics
We used AxoGraph 4.9 on a Macintosh G4 computer for data analysis. For quantification of SK currents, we integrated the currents beginning at the peak of the response (ca. 5 ms following the end of a voltage command pulse, see e.g., Fig. 3C). Results are reported as means ± SE. For all experiments, statistical significance was determined using ANOVA with repeated measures and Student's t-test within Microsoft EXCEL (Richmond, WA). Significance was set at P 
0.05.
|
|
RESULTS |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
We first were interested in identifying cells in the olfactory bulb that express SK currents. To isolate SK currents, we blocked transmitter and voltage-gated channels with NBQX, AP5, gabazine, TTX, and TEA. Under these conditions, somatic depolarizing steps of mitral cells (+10 mV, 100 ms) produced long-lasting outward currents (12.3 ± 1.1 pC, n = 27) that were inhibited by the SK channel antagonist 100 nM apamin (2.5 ± 0.4 pC (n = 18); P < 0.0001 Fig. 1). In granule cells, the same protocol did not evoke an outward current (n = 6, Fig. 1C). Likewise, apamin had no effect on granule cells. Thus mitral cells, but not granule cells express SK currents. Calcium-activated potassium channels can couple with several classes of voltage-gated calcium channels (Davies et al. 1996; Marrion and Tavalin 1998; Prakriya and Lingle 1999; Wolfart et al. 2001; Womack et al. 2004). As shown in Fig. 1B, the nonselective calcium channel blocker Cd2+ (200 µM) strongly inhibited slow outward currents in mitral cells [2.98 ± 0.5 pC (n = 9); P < 0.001 Fig. 1D]. Co-application of Cd2+ and apamin did not produce any further inhibition, indicating that Cd2+ completely blocked the apamin-sensitive current under these conditions. Thus voltage-activated calcium channels are a primary source of Ca2+ for SK channel activation.
|
Although 100 ms pulses are typically used to evoke SK currents, the duration and pattern of depolarization will affect the calcium entry and thus the activation of SK channels. In particular, the duration of action potentials in neurons is much briefer, ca. 2 ms. As expected, the apamin-sensitive current decreased as we decreased the duration of the step depolarization (Fig. 2). However, even 2 ms depolarizations activated apamin-sensitive currents [1.86 ± 0.25 pC (n = 6); Fig. 2B]. Thus single backpropagating action potentials may be sufficient to activate SK channels in mitral cell dendrites.
|
; Nicoll and Jahr 1982), resulting in activation of dendritic AMPA and NMDA autoreceptors (Friedman and Strowbridge 2000; Schoppa and Westbrook 2002). NMDA autoreceptor currents can last hundreds of milliseconds and are an additional source of dendritic calcium transients (Isaacson 1999). We reasoned that currents evoked by depolarizing step pulses should contain SK as well as NMDA autoreceptor components. To more easily detect NMDA autoreceptor currents, we lowered extracellular Mg2+ to 100 µM. Under these conditions, currents evoked by 100 ms voltage steps often had a prominent shoulder that followed the peak response (Fig. 3A, control). Apamin blocked the outward current and unmasked an underlying inward current (Fig. 3A, apamin) that was subsequently blocked by AP5, consistent with an NMDA autoreceptor current (Fig. 3A1, AP5 + apamin). Similar results were obtained with 2 ms voltage steps (Fig. 3B). Interestingly, both the SK and NMDA autoreceptor currents had similar durations. When measured after a 100 ms voltage step, the duration for the SK current was 337 ± 38 and 449.2 ± 97 ms for the NMDA autoreceptor current. Thus under these conditions the NMDA current was obscured by the outward SK current. We next examined whether NMDA autoreceptor-mediated calcium influx could activate SK channels in mitral cells. Block of the autoreceptor current with AP5 actually increased the net outward current evoked by 100 ms voltage steps (Fig. 4A), Therefore Ca2+ influx via NMDA autoreceptors is not necessary for full activation of SK channels. Rather, calcium entry through voltage-activated Ca2+ channels appears sufficient. However currents evoked by 2 ms voltage steps were decreased by AP5 [49 ± 9% of control (n = 8); Fig. 4, B and C]. As the duration of voltage step increased AP5 increased the apamin-sensitive current, suggesting that the contribution of autoreceptors to SK activation was overwhelmed by calcium influx through voltage-gated Ca2+ channels (Fig. 4C). In physiological concentrations of Mg2+ (1 mM), SK channel activation with 2 ms voltage steps was not affected by AP5 [110 ± 10% of control (n = 8); P = 0.65], consistent with the small amplitude of the NMDA autoreceptor current under these conditions.
|
The afterhyperpolarizations due to SK channels have prominent effects on cell firing (Sah and Faber 2002; Vergara et al. 1998). We thus examined mitral cell firing patterns after blocking synaptic receptors with NBQX, AP5, and gabazine. Long depolarizing current pulses (3 s) were used to produce trains of action potentials that showed relatively little accommodation. As previously shown in rat olfactory bulb (Friedman and Strowbridge 2000), a reduction in extracellular Ca2+ increased the firing frequency in action potential trains [278 ± 65% of control (n = 3); Fig. 5A]. Reduction of extracellular Ca2+ also reduced the afterhyperpolarization following each action potential in the train (Fig. 5A). As shown in Fig. 6A, apamin had a similar effect as low extracellular Ca2+ on firing frequency in action potential trains [214 ± 27% of control (n = 9)], suggesting that SK channels regulate cell firing in mitral cells. In the absence of AP5, the NMDA autoreceptor response could be seen as a persistent depolarization at the end of the current pulse (Fig. 6A, inset). However, AP5 did not influence the effect of apamin on cell firing, consistent with voltage-clamp recordings that showed little SK activation by NMDA autoreceptors in physiological Mg2+. Apamin had no effect on action potential frequency in granule cells (107 ± 8% of control; P = 0.41, n = 5 Fig. 6B).
|
|
). Apamin strongly increased dendrodendritic inhibition (Fig. 7A2) regardless of the extracellular Mg2+ concentration: 170 ± 10% of control in 1 mM Mg2+ (n = 6); 193 ± 34% of control in 0.1 mM Mg2+ (n = 10; Fig. 7B). As in previous studies (Schoppa et al. 1998), application of AP5 considerably reduced dendrodendritic inhibition in all neurons tested [44 ± 22% of control in 1 mM Mg2+ (n = 5); 12 ± 5% of control in 0.1 mM Mg2+ (n = 4); Fig. 7B].
|
|
DISCUSSION |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
Our results indicate that an apamin-sensitive current is present in mitral cells of the olfactory bulb. By reducing action potential frequency in mitral cells, the SK current can dampen inhibition driven by dendrodendritic synapses with granule cells. Apamin-sensitive currents are present in neurons in many brain regions, in agreement with in situ hybridization and immunohistochemical studies depicting a partially overlapping but distinct distribution pattern of SK subunits (Gehlert and Gackenheimer 1993; Sailer et al. 2004; Stocker and Pedarzani 2000). Calcium entry during action potentials activates SK channels that underlie a component of the afterhyperpolarization, thus positioning these current to control the frequency and regularity of action potentials.
Depending on the neuronal subtype and combination of SK channel subunits, SK channels contribute to distinct patterns of neuronal output including the shaping of synaptic responses as well as rhythmic and burst firing (Bond et al. 2005). For example, in dopaminergic midbrain neurons that are tonically active, block of SK channels increases spontaneous action potentials and also reduces the precision of evoked action potential trains (Wolfart et al. 2001). In the subthalamic nucleus, apamin converts tonic action potential firing into burst activity (Hallworth et al. 2003). SK channels contribute to rhythmic bursting activity early in the development of Purkinje cells (Cingolani et al. 2002). Consistent with the presence of SK currents, mitral cells showed the expected loss of the afterhyperpolarization and increased evoked firing in the presence of apamin. However, natural stimuli cause bursts of action potentials in mitral cells locked to the respiratory cycle (Cang and Isaacson 2003), thus SK currents may also affect these more complex firing patterns.
SK channel subunits
In the olfactory bulb, SK1 and SK3 are highly expressed in the glomerulus and the EPL with SK2 expressed at lower levels in these layers. None of the subunits are highly expressed in the granule cell layer (Sailer et al. 2004). This pattern is consistent with the presence of robust SK current in mitral cells but not in granule cells. The strong immunostaining pattern in the EPL and glomerular layer could indicate that SK channels are preferentially expressed in mitral cell dendrites, although our data do not directly address the distribution of SK channels in mitral cells. In the neocortex and hippocampus, SK1 and SK2 appear to be expressed in pyramidal cell dendrites, whereas SK3 has been reported to be located in hippocampal presynaptic terminals (Obermair et al. 2003; Sailer et al. 2002). Recently, transfected myc-tagged SK2 subunits have been localized to dendritic spines in cultured hippocampal neurons (Ngo-Anh et al. 2005). The prominent staining of SK3 in the EPL (Sailer et al. 2004) suggests that the expression of SK subunits in mitral cells may differ from hippocampal pyramidal cells. This may not be surprising given the distinctive morphology of mitral cell dendrites that lack spines and have presynaptic glutamate release sites.
SK channels and NMDA autoreceptors
The dendritic release of glutamate from mitral cells activates not only the postsynaptic granule cells but also glutamate receptors on the surrounding dendritic membrane. This can be observed after block of dendrodendritic inhibition as a long-lasting excitation of mitral cells (Nicoll and Jahr 1982; Nowycky et al. 1981) due to activation of dendritic NMDA autoreceptors (Isaacson 1999). Our results indicate NMDA autoreceptor currents and SK currents have an overlapping time course after membrane depolarization.
This situation suggests an interaction between NMDA autoreceptor currents and SK channel currents in controlling action potential patterning. SK currents could shunt autoreceptor-mediated depolarization of dendrites without altering NMDA receptor-mediated calcium influx, the latter of which may be important for cell signaling or transmitter release. Block of NMDA receptors reduces the frequency and clustering of action potentials evoked in mitral cells (Friedman and Strowbridge 2000), suggesting that NMDA autoreceptors can increase dendritic excitability.
It was initially suggested that SK channels are tightly coupled to L-type calcium channels (Marrion and Tavalin 1998). However, it is now clear that multiple calcium channel subtypes and calcium sources can activate SK channels in certain situations (Stocker 2004). Ca2+ influx through NMDA receptors can also activate calcium-activated potassium channels. BK-type Ca2+-activated potassium channels can be directly activated by NMDA receptors in granule cells of the olfactory bulb (Isaacson and Murphy 2001). In hippocampal pyramidal cells, long somatic application of NMDA can evoke SK currents (Sah and Faber 2002). This action can be quite localized because NMDA receptor-dependent activation of SK channels in CA1 dendritic spines can reduce evoked excitatory postsynaptic potentials (EPSPs) (Ngo-Anh et al. 2005). Likewise in the amygdala SK channels, activated by Ca2+ influx through synaptically activated NMDA receptors, reduced EPSPs and the resulting LTP (Faber et al. 2005). In our experiments using somatic recording, we were only able to detect an NMDA receptor contribution to SK channel activation under some conditions (low Mg2+, brief voltage steps). However, this does not exclude local activation of SK channels by NMDA autoreceptors under physiological conditions.
SK channels and dendritic excitability
Dendritic excitability is regulated by the cell-specific complement of voltage- and ligand-gated ion channels distributed across dendritic compartments (Hausser et al. 2000). In mitral cells, action potentials attenuate in lateral dendrites, but not in apical dendrites, as a result of A-type potassium currents as well as GABAergic inhibition (Christie and Westbrook 2003; Lowe 2002; Margrie et al. 2001; Xiong and Chen 2002). Focal application of apamin onto apical dendrites of CA1 pyramidal neurons increases the duration of dendritic plateau potentials (Cai et al. 2004), suggesting that SK channels can regulate local excitability. In this case, A and SK currents have distinctive effects on plateau potentials, perhaps reflecting their different biophysical characteristics. Because mitral cell dendrites reliably transmit backpropagating action potentials that are necessary for glutamate release, SK channels likely have a dual role in regulating dendritic excitability and transmitter release.
|
GRANTS |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
|
ACKNOWLEDGMENTS |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
|
FOOTNOTES |
|---|
Address for reprint requests and other correspondence: G. L. Westbrook, Vollum Institute, Oregon Health and Science University L474, Portland OR, 97239 (E-mail: westbroo{at}ohsu.edu)
|
REFERENCES |
|---|
|
TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
|---|
Bond CT, Maylie J, and Adelman JP. SK channels in excitability pacemaking and synaptic integration. Curr Opin Neurobiol 15: 305–311, 2005.[CrossRef][ISI][Medline]
Cai X, Liang CW, Muralidharan S, Kao JP, Tang CM, and Thompson SM. Unique roles of SK and Kv4.2 potassium channels in dendritic integration. Neuron 44: 351–364, 2004.[CrossRef][ISI][Medline]
Cang J and Isaacson JS. In vivo whole-cell recording of odor-evoked synaptic transmission in the rat olfactory bulb. J Neurosci 23: 4108–4116, 2003.
Chen WR, Midtgaard J, and Shepherd GM. Forward and backward propagation of dendritic impulses and their synaptic control in mitral cells. Science 278: 463–467, 1997.
Christie JM and Westbrook GL. Regulation of backpropagating action potentials in mitral cell lateral dendrites by A-type potassium currents. J Neurophysiol 89: 2466–2472, 2003.
Cingolani LA, Gymnopoulos M, Boccaccio A, Stocker M, and Pedarzani P. Developmental regulation of small-conductance Ca2+-activated K+ channel expression and function in rat Purkinje neurons. J Neurosci 22: 4456–4467, 2002.
Davies PJ, Ireland DR, and McLachlan EM. Sources of Ca2+ for different Ca(2+)-activated K+ conductances in neurones of the rat superior cervical ganglion. J Physiol 495: 353–366, 1996.[ISI][Medline]
Davison IG, Boyd JD, and Delaney KR. Dopamine inhibits mitral/tufted- granule cell synapses in the frog olfactory bulb. J Neurosci 24: 8057–8067, 2004.
Faber ES, Delaney AJ, and Sah P. SK channels regulate excitatory synaptic transmission and plasticity in the lateral amygdala. Nat Neurosci 8: 635–641, 2005.[CrossRef][ISI][Medline]
Friedman D and Strowbridge BW. Functional role of NMDA autoreceptors in olfactory mitral cells. J Neurophysiol 84: 39–50, 2000.
Gehlert DR and Gackenheimer SL. Comparison of the distribution of binding sites for the potassium channel ligands [125I]apamin, [125I]charybdotoxin and [125I]iodoglyburide in the rat brain. Neuroscience 52: 191–205, 1993.[CrossRef][ISI][Medline]
Hallworth NE, Wilson CJ, and Bevan MD. Apamin-sensitive small conductance calcium-activated potassium channels, through their selective coupling to voltage-gated calcium channels, are critical determinants of the precision, pace, and pattern of action potential generation in rat subthalamic nucleus neurons in vitro. J Neurosci 23: 7525–7542, 2003.
Hausser M, Spruston N, and Stuart GJ. Diversity and dynamics of dendritic signaling. Science 290: 739–744, 2000.
Isaacson JS. Glutamate spillover mediates excitatory transmission in the rat olfactory bulb. Neuron 23: 377–384, 1999.[CrossRef][ISI][Medline]
Isaacson JS and Murphy GJ. Glutamate-mediated extrasynaptic inhibition: direct coupling of NMDA receptors to Ca(2+)-activated K+ channels. Neuron 31: 1027–1034, 2001.[CrossRef][ISI][Medline]
Isaacson JS and Strowbridge BW. Olfactory reciprocal synapses: dendritic signaling in the CNS. Neuron 20: 749–761, 1998.[CrossRef][ISI][Medline]
Lowe G. Inhibition of backpropagating action potentials in mitral cell secondary dendrites. J Neurophysiol 88: 64–85, 2002.
Margrie TW, Sakmann B, and Urban NN. Action potential propagation in mitral cell lateral dendrites is decremental and controls recurrent and lateral inhibition in the mammalian olfactory bulb. Proc Natl Acad Sci USA 98: 319–324, 2001.
Marrion NV and Tavalin SJ. Selective activation of Ca2+-activated K+ channels by co-localized Ca2+ channels in hippocampal neurons. Nature 395: 900–905, 1998.[CrossRef][Medline]
Montague AA and Greer CA. Differential distribution of ionotropic glutamate receptor subunits in the rat olfactory bulb. J Comp Neurol 405: 233–246, 1999.[CrossRef][ISI][Medline]
Ngo-Anh TJ, Bloodgood BL, Lin M, Sabatini BL, Maylie J, and Adelman JP. SK channels and NMDA receptors form a Ca(2+)-mediated feedback loop in dendritic spines. Nat Neurosci 8: 642–649, 2005.[CrossRef][ISI][Medline]
Nicoll RA and Jahr CE. Self-excitation of olfactory bulb neurones. Nature 296: 441–444, 1982.[CrossRef][Medline]
Nowycky MC, Mori K, and Shepherd GM. GABAergic mechanisms of dendrodendritic synapses in isolated turtle olfactory bulb. J Neurophysiol 46: 639–648, 1981.
Obermair GJ, Kaufmann WA, Knaus HG, and Flucher BE. The small conductance Ca2+-activated K+ channel SK3 is localized in nerve terminals of excitatory synapses of cultured mouse hippocampal neurons. Eur J Neurosci 17: 721–731, 2003.[CrossRef][ISI][Medline]
Petralia RS, Wang YX, and Wenthold RJ. The NMDA receptor subunits NR2A and NR2B show histological and ultrastructural localization patterns similar to those of NR1. J Neurosci 14: 6102–6120, 1994.[Abstract]
Prakriya M and Lingle CJ. BK channel activation by brief depolarizations requires Ca2+ influx through L- and Q-type Ca2+ channels in rat chromaffin cells. J Neurophysiol 81: 2267–2278, 1999.
Sah P and Faber ES. Channels underlying neuronal calcium-activated potassium currents. Prog Neurobiol 66: 345–353, 2002.[CrossRef][ISI][Medline]
Sailer CA, Hu H, Kaufmann WA, Trieb M, Schwarzer C, Storm JF, and Knaus HG. Regional differences in distribution and functional expression of small-conductance Ca2+-activated K+ channels in rat brain. J Neurosci 22: 9698–9707, 2002.
Sailer CA, Kaufmann WA, Marksteiner J, and Knaus HG. Comparative immunohistochemical distribution of three small-conductance Ca2+-activated potassium channel subunits, SK1, SK2, and SK3 in mouse brain. Mol Cell Neurosci 26: 458–469, 2004.[CrossRef][ISI][Medline]
Schoppa NE, Kinzie JM, Sahara Y, Segerson TP, and Westbrook GL. Dendrodendritic inhibition in the olfactory bulb is driven by NMDA receptors. J Neurosci 18: 6790–6802, 1998.
Schoppa NE and Westbrook GL. AMPA autoreceptors drive correlated spiking in olfactory bulb glomeruli. Nat Neurosci 5: 1194–1202, 2002.[CrossRef][ISI][Medline]
Stocker M and Pedarzani P. Differential distribution of three Ca(2+)-activated K(+) channel subunits, SK1, SK2, and SK3, in the adult rat central nervous system. Mol Cell Neurosci 15: 476–493, 2000.[CrossRef][ISI][Medline]
Stocker M. Ca(2+)-activated K+ channels: molecular determinants and function of the SK family. Nat Rev Neurosci 5: 758–770, 2004.[CrossRef][ISI][Medline]
Stuart GJ, Dodt HU, and Sakmann B. Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pfluegers 423: 511–518, 1993.
Vergara C, Latorre R, Marrion NV, and Adelman JP. Calcium-activated potassium channels. Curr Opin Neurobiol 8: 321–329, 1998.[CrossRef][ISI][Medline]
Wolfart J, Neuhoff H, Franz O, and Roeper J. Differential expression of the small-conductance, calcium-activated potassium channel SK3 is critical for pacemaker control in dopaminergic midbrain neurons. J Neurosci 21: 3443–3456, 2001.
Womack MD, Chevez C, and Khodakhah K. Calcium-activated potassium channels are selectively coupled to P/Q-type calcium channels in cerebellar Purkinje neurons. J Neurosci 24: 8818–8822, 2004.
Xiong W and Chen WR. Dynamic gating of spike propagation in the mitral cell lateral dendrites. Neuron 34: 115–126, 2002.[CrossRef][ISI][Medline]
This article has been cited by other articles:
|
|
 |
|
  S. Liu and M. T. Shipley Multiple Conductances Cooperatively Regulate Spontaneous Bursting in Mouse Olfactory Bulb External Tufted Cells J. Neurosci., February 13, 2008; 28(7): 1625 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Teagarden, J. F. Atherton, M. D. Bevan, and C. J. Wilson Accumulation of cytoplasmic calcium, but not apamin-sensitive afterhyperpolarization current, during high frequency firing in rat subthalamic nucleus cells J. Physiol., February 1, 2008; 586(3): 817 - 833. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
