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EDITORIAL FOCUS
One popular idea is that elevated serotonin associated with SSRI treatment downregulates expression of 5-HT1A receptors that mediate autoinhibitory feedback on serotonergic terminals and cell body regions. This is quite reasonable, as numerous G-protein-coupled receptors have been shown to undergo desensitization-induced internalization (Claing et al. 2002
). Indeed, depressed patients undergoing SSRI treatment show lower 5-HT1A levels than similarly depressed individuals that have not begun therapeutic treatment (Parsey et al. 2006). In rodent models, there is also strong support for similar effects of prolonged SSRI exposure, but most previous investigations used biochemical assays or radioligand binding to assess changes in receptor function or expression, methods that lack cellular resolution (e.g. Blier et al. 1998; Elena Castro et al. 2003; Pejchal et al. 2002). Another important feature of these earlier studies is that they did not examine the effects of SSRI administration in animal models of depression.
In this issue of the Journal of Neurophysiology (p. 196–204), Cornellisse and colleagues report intriguing observations on the effects of SSRI treatment using cellular assays of 5-HT1A receptor responses in serotonergic neurons of the dorsal raphe nucleus (DRN). These studies involved electrophysiological recordings from identified serotonergic neurons in brain slices taken from control rats, and from rats subjected to social stress, as a model of anhedonia. These socially stressed animals exhibited diminished anticipation of sucrose reward, as reported previously (Von Frijtag et al. 2000), but the authors found no evidence for reduced 5-HT1A receptor expression in DRN. This is consistent with the notably mixed reports on the link between 5-HT1A expression levels and depression in humans (without SSRI treatment), where some studies report enhanced receptor expression in depressed subjects (Stockmeier et al. 1998) and others report decreased expression (Arango et al. 2001).
The most exciting observation from Cornelisse et al. was that only animals chronically treated with the SSRI fluoxetine showed a decreased 5HT1A response but not through receptor downregulation. Decreased responses to either 5HT1A or GABAB agonists were seen in DRN neurons from fluoxetine-treated animals. Both these receptor classes inhibit excitability through G proteins that activate inwardly rectifying K+ channels (GIRK) to hyperpolarize the membrane potential and inhibit activity (Bayliss et al. 1997; Innis and Aghajanian 1987; Innis et al. 1988; Williams et al. 1988). Both these receptor classes were inhibited in a similar manner. Perhaps surprisingly, the effects occurred in both control and socially stressed animals that were subjected to SSRI treatment and was thus independent of the depressed state of the animals.
These findings suggest a divergence in the mechanisms underlying the etiology of depression and effective pharmacological treatment. The lack of evidence for changes in 5-HT1A expression in anhedonic animals suggests that this receptor is not principally involved in the depressive phenotype. In contrast, treatment of depression with SSRIs does indeed affect signaling via this receptor class by altering the expression of GIRK channels in serotonergic neurons that couple activation of 5-HT1A and GABAB receptors to decreases in excitability. By downregulating GIRK rather than one specific receptor, fluoxetine parsimoniously reduces the inhibitory inputs from a number of metabotropic receptors and increases the excitability of dorsal raphe neurons, which in turn presumably increases release of serotonin, the modern panacea. Whether this study has identified novel therapeutic targets, remains to be established, but it does suggest an intriguing area for further investigation.
Department of Anesthesia and Critical Care, Committee on Neurobiology, Committee on Cell Physiology, University of Chicago, Chicago, Illinois
Address reprint requests and other correspondence to: Dept. of Anesthesia and Critical Care, Committee on Neurobiology, Committee on Cell Physiology, University of Chicago, 5841 S. Maryland Ave., MC4028, Chicago, IL 60637 (E-mail:dmcgehee{at}uchicago.edu)
REFERENCES
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Stockmeier CA, Shapiro LA, Dilley GE, Kolli TN, Friedman L, Rajkowska G. Increase in serotonin-1A autoreceptors in the midbrain of suicide victims with major depression-postmortem evidence for decreased serotonin activity. J Neurosci 18: 7394–7401, 1998.
Von Frijtag JC, Reijmers LG, Van der Harst JE, Leus IE, Van den Bos R, Spruijt BM. Defeat followed by individual housing results in long-term impaired reward- and cognition-related behaviours in rats. Behav Brain Res 117: 137–146, 2000.[CrossRef][Web of Science][Medline]
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