JN Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Neurophysiol (April 2, 2003). doi:10.1152/jn.00070.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
90/2/771    most recent
00070.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Myme, C. I.
Right arrow Articles by Nelson, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Myme, C. I.
Right arrow Articles by Nelson, S. B.
Submitted on January 27, 2003
Accepted on March 28, 2003

THE NMDA TO AMPA RATIO AT SYNAPSES ONTO LAYER 2/3 PYRAMIDAL NEURONS IS CONSERVED ACROSS PREFRONTAL AND VISUAL CORTICES

Chaelon I. Myme, Ken Sugino, Gina G. Turrigiano, and Sacha B. Nelson*

* To whom correspondence should be addressed. E-mail: nelson{at}brandeis.edu.

In order to better understand regulation of NMDA and AMPA receptor complements across the cortex, and to investigate NMDAR-based models of persistent activity, we compared NMDA/AMPA ratios in prefrontal (PFC) and visual cortex (VC) in rat. Whole cell voltage clamp responses were recorded in brain slices from layer 2/3 pyramidal cells of the medial PFC and VC of rats age p16-p21. Mixed mEPSCs having AMPAR and NMDAR mediated components were isolated in nominally 0 Mg++ ACSF. Averaged mEPSCs were well-fit by double exponentials. No significant differences in the NMDA/AMPA ratio (PFC: 27% ± 1%; VC: 28% ± 3%), peak mEPSC amplitude (PFC: 19.1 ± 1 pA; VC: 17.5 ± 0.7 pA), NMDAR decay kinetics (PFC: 69 ± 8 ms; VC: 67 ± 6 ms), or degree of correlation between NMDAR and AMPAR mediated mEPSC components, were found between the areas (PFC: n = 27; VC: n = 28). Recordings from older rats (p26-29) also showed no differences. EPSCs were evoked extracellularly in 2 mM Mg++ at depolarized potentials; although the average NMDA/AMPA ratio was larger than that observed for mEPSCs, the ratio was similar in the two regions. In nominally 0 Mg++ and in the presence of CNQX, spontaneous activation of NMDAR increased recording noise and produced a small tonic depolarization which was similar in both areas. We conclude that this basic property of excitatory transmission is conserved across PFC and VC synapses, and is therefore unlikely to contribute to differences in firing patterns observed in vivo in the two regions.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
S. K. Esser, S. Hill, and G. Tononi
Breakdown of Effective Connectivity During Slow Wave Sleep: Investigating the Mechanism Underlying a Cortical Gate Using Large-Scale Modeling
J Neurophysiol, October 1, 2009; 102(4): 2096 - 2111.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Polsky, B. Mel, and J. Schiller
Encoding and Decoding Bursts by NMDA Spikes in Basal Dendrites of Layer 5 Pyramidal Neurons
J. Neurosci., September 23, 2009; 29(38): 11891 - 11903.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
M. Stimberg, K. Wimmer, R. Martin, L. Schwabe, J. Marino, J. Schummers, D. C. Lyon, M. Sur, and K. Obermayer
The Operating Regime of Local Computations in Primary Visual Cortex
Cereb Cortex, September 1, 2009; 19(9): 2166 - 2180.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. David, L. P. Cacheaux, S. Ivens, E. Lapilover, U. Heinemann, D. Kaufer, and A. Friedman
Astrocytic Dysfunction in Epileptogenesis: Consequence of Altered Potassium and Glutamate Homeostasis?
J. Neurosci., August 26, 2009; 29(34): 10588 - 10599.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B.-J. Yoon, G. B. Smith, A. J. Heynen, R. L. Neve, and M. F. Bear
Essential role for a long-term depression mechanism in ocular dominance plasticity
PNAS, June 16, 2009; 106(24): 9860 - 9865.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
F. Espinosa and E. T. Kavalali
NMDA Receptor Activation by Spontaneous Glutamatergic Neurotransmission
J Neurophysiol, May 1, 2009; 101(5): 2290 - 2296.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y. Pilpel, A. Kolleker, S. Berberich, M. Ginger, A. Frick, E. Mientjes, B. A. Oostra, and P. H. Seeburg
Synaptic ionotropic glutamate receptors and plasticity are developmentally altered in the CA1 field of Fmr1 knockout mice
J. Physiol., February 15, 2009; 587(4): 787 - 804.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. N. Miller, B. W. Okaty, and S. B. Nelson
Region-Specific Spike-Frequency Acceleration in Layer 5 Pyramidal Neurons Mediated by Kv1 Subunits
J. Neurosci., December 17, 2008; 28(51): 13716 - 13726.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Wang, G. G. Stradtman III, X.-J. Wang, and W.-J. Gao
A specialized NMDA receptor function in layer 5 recurrent microcircuitry of the adult rat prefrontal cortex
PNAS, October 28, 2008; 105(43): 16791 - 16796.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Z. Yang, M. Krause, G. Rao, B. L. McNaughton, and C. A. Barnes
Synaptic Commitment: Developmentally Regulated Reciprocal Changes in Hippocampal Granule Cell NMDA and AMPA Receptors Over the Lifespan
J Neurophysiol, June 1, 2008; 99(6): 2760 - 2768.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. T. Moyer, J. A. Wolf, and L. H. Finkel
Effects of Dopaminergic Modulation on the Integrative Properties of the Ventral Striatal Medium Spiny Neuron
J Neurophysiol, December 1, 2007; 98(6): 3731 - 3748.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Sarid, R. Bruno, B. Sakmann, I. Segev, and D. Feldmeyer
Modeling a layer 4-to-layer 2/3 module of a single column in rat neocortex: Interweaving in vitro and in vivo experimental observations
PNAS, October 9, 2007; 104(41): 16353 - 16358.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. N. Blythe, J. F. Atherton, and M. D. Bevan
Synaptic Activation of Dendritic AMPA and NMDA Receptors Generates Transient High-Frequency Firing in Substantia Nigra Dopamine Neurons In Vitro
J Neurophysiol, April 1, 2007; 97(4): 2837 - 2850.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Kepecs and S. Raghavachari
Gating Information by Two-State Membrane Potential Fluctuations
J Neurophysiol, April 1, 2007; 97(4): 3015 - 3023.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
N. Origlia, N. Kuczewski, E. Aztiria, D. Gautam, J. Wess, and L. Domenici
Muscarinic acetylcholine receptor knockout mice show distinct synaptic plasticity impairments in the visual cortex
J. Physiol., December 15, 2006; 577(3): 829 - 840.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
N. S. Desai, T. M. Casimiro, S. M. Gruber, and P. W. Vanderklish
Early Postnatal Plasticity in Neocortex of Fmr1 Knockout Mice
J Neurophysiol, October 1, 2006; 96(4): 1734 - 1745.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Rhodes
The properties and implications of NMDA spikes in neocortical pyramidal cells.
J. Neurosci., June 21, 2006; 26(25): 6704 - 6715.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. V. Rangan, D. Cai, and D. W. McLaughlin
Inaugural Article: Modeling the spatiotemporal cortical activity associated with the line-motion illusion in primary visual cortex
PNAS, December 27, 2005; 102(52): 18793 - 18800.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. A. Wolf, J. T. Moyer, M. T. Lazarewicz, D. Contreras, M. Benoit-Marand, P. O'Donnell, and L. H. Finkel
NMDA/AMPA Ratio Impacts State Transitions and Entrainment to Oscillations in a Computational Model of the Nucleus Accumbens Medium Spiny Projection Neuron
J. Neurosci., October 5, 2005; 25(40): 9080 - 9095.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Cai, A. V. Rangan, and D. W. McLaughlin
Architectural and synaptic mechanisms underlying coherent spontaneous activity in V1
PNAS, April 19, 2005; 102(16): 5868 - 5873.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. C. Kotak, S. Fujisawa, F. A. Lee, O. Karthikeyan, C. Aoki, and D. H. Sanes
Hearing Loss Raises Excitability in the Auditory Cortex
J. Neurosci., April 13, 2005; 25(15): 3908 - 3918.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
P. J. Sjostrom, G. G. Turrigiano, and S. B. Nelson
Endocannabinoid-Dependent Neocortical Layer-5 LTD in the Absence of Postsynaptic Spiking
J Neurophysiol, December 1, 2004; 92(6): 3338 - 3343.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. B. Mierau, R. M. Meredith, A. L. Upton, and O. Paulsen
Dissociation of experience-dependent and -independent changes in excitatory synaptic transmission during development of barrel cortex
PNAS, October 26, 2004; 101(43): 15518 - 15523.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. M. de la Prida and B. Gal
Synaptic Contributions to Focal and Widespread Spatiotemporal Dynamics in the Isolated Rat Subiculum In Vitro
J. Neurosci., June 16, 2004; 24(24): 5525 - 5536.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. W. Hendricson, J. R. Sibbald, and R. A. Morrisett
Ethanol Alters the Frequency, Amplitude, and Decay Kinetics of Sr2+-Supported, Asynchronous NMDAR mEPSCs in Rat Hippocampal Slices
J Neurophysiol, June 1, 2004; 91(6): 2568 - 2577.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Kattenstroth, E. Tantalaki, T. C. Sudhof, K. Gottmann, and M. Missler
Postsynaptic N-methyl-D-aspartate receptor function requires {alpha}-neurexins
PNAS, February 24, 2004; 101(8): 2607 - 2612.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2003 by the The American Physiological Society.