JN Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Neurophysiol (June 30, 2004). doi:10.1152/jn.00270.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
93/1/94    most recent
00270.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wong, K. Y
Right arrow Articles by Dowling, J. E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, K. Y
Right arrow Articles by Dowling, J. E
Submitted on March 18, 2004
Accepted on June 23, 2004

Retinal Bipolar Cell Input Mechanisms in Giant Danio: II. Patch-Clamp Analysis of ON Bipolar Cells

Kwoon Y Wong1*, Ethan D Cohen1, and John E Dowling1

1 Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA

* To whom correspondence should be addressed. E-mail: Kwoon_Wong{at}brown.edu.

Glutamate receptors on giant danio retinal ON bipolar cells were studied with whole-cell patch clamping using a slice preparation. Cone-driven ON bipolars (Cb's) and mixed-input ON bipolars (Mb's) were identified morphologically. Most Cb's responded to the excitatory amino acid transporter (EAAT) substrate D-aspartate but not to the group III metabotropic glutamate receptor (mGluR) agonist L-AP4 or the AMPA/kainate receptor agonist kainate, suggesting EAATs are the primary glutamate receptors on Cb's. The EAAT inhibitor TBOA blocked all light-evoked responses of Cb's, suggesting these responses are mediated exclusively by EAATs. Conversely, all Mb's responded to D-aspartate and L-AP4 but not to kainate, indicating they have both EAATs and mGluRs (presumably mGluR6). The light responses of Mb's involve both receptors, since they could be blocked by TBOA plus CPPG (a group III mGluR antagonist) but not by either alone. Under dark-adapted conditions, the responses of Mb's to green (rod-selective) stimuli were reduced by CPPG but enhanced by TBOA. In contrast, both antagonists reduced the responses to red (cone-selective) stimuli, although TBOA was more effective. Furthermore, under photopic conditions, TBOA failed to eliminate light-evoked responses of Mb's. Thus, on Mb's, rod inputs are mediated predominantly by mGluR6, whereas cone inputs are mediated mainly by EAATs but also by mGluR6 to some extent. Finally, we explored the interactions between EAATs and mGluR6 in Mb's. Responses to D-aspartate were reduced by L-AP4, and vice versa. Therefore, mGluR6 and EAATs suppress each other, and this might underlie mutual suppression between rod and cone signals in Mb's.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
Y. Ping, H. Huang, X.-J. Zhang, and X.-L. Yang
Melatonin potentiates rod signals to ON type bipolar cells in fish retina
J. Physiol., June 1, 2008; 586(11): 2683 - 2694.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Emran, J. Rihel, A. R. Adolph, K. Y. Wong, S. Kraves, and J. E. Dowling
OFF ganglion cells cannot drive the optokinetic reflex in zebrafish
PNAS, November 27, 2007; 104(48): 19126 - 19131.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. Y. Wong, F. A. Dunn, D. M. Graham, and D. M. Berson
Synaptic influences on rat ganglion-cell photoreceptors
J. Physiol., July 1, 2007; 582(1): 279 - 296.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D.-Q. Zhang, T.-R. Zhou, and D. G. McMahon
Functional Heterogeneity of Retinal Dopaminergic Neurons Underlying Their Multiple Roles in Vision
J. Neurosci., January 17, 2007; 27(3): 692 - 699.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
E. Wersinger, Y. Schwab, J.-A. Sahel, A. Rendon, D. V. Pow, S. Picaud, and M. J. Roux
The glutamate transporter EAAT5 works as a presynaptic receptor in mouse rod bipolar cells
J. Physiol., November 15, 2006; 577(1): 221 - 234.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. I. Matsui, A. L. Egana, T. R. Sponholtz, A. R. Adolph, and J. E. Dowling
Effects of ethanol on photoreceptors and visual function in developing zebrafish.
Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4589 - 4597.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Vigh and H. von Gersdorff
Prolonged Reciprocal Signaling via NMDA and GABA Receptors at a Retinal Ribbon Synapse
J. Neurosci., December 7, 2005; 25(49): 11412 - 11423.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Y. Wong and J. E. Dowling
Retinal Bipolar Cell Input Mechanisms in Giant Danio. III. ON-OFF Bipolar Cells and Their Color-Opponent Mechanisms
J Neurophysiol, July 1, 2005; 94(1): 265 - 272.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. P. Dryja, T. L. McGee, E. L. Berson, G. A. Fishman, M. A. Sandberg, K. R. Alexander, D. J. Derlacki, and A. S. Rajagopalan
Night blindness and abnormal cone electroretinogram ON responses in patients with mutations in the GRM6 gene encoding mGluR6
PNAS, March 29, 2005; 102(13): 4884 - 4889.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2004 by the The American Physiological Society.