A key element of walking is the coordinated interplay of multiple limbs to achieve a stable locomotor pattern adapted to the environment. We investigated intersegmental coordination of walking in the stick insect, Carausius morosus by examining the influence a single stepping leg has on the motoneural activity of the other hemiganglia, and potential changes with walking direction. We used a reduced single leg walking preparation with only one intact front, middle or hind leg. The intact leg performed stepping movements on a treadmill, hence providing intersegmental signals about its stepping to the other hemiganglia. The activity of coxal motoneurons was recorded extracellularly in all other segments. Stepping sequences of all legs in either walking direction were accompanied by an increase in coxal motoneuron (MN) activity of all other segments which was modulated and slightly in phase with stance of the walking leg. In addition, forward stepping of the front leg and, to a lesser extent, backward stepping of the hind leg, elicited alternating activity in mesothoracic coxal MNs. Forward and backward stepping of the middle leg did not elicit alternating activity in coxal MNs in any other hemiganglia, indicating that the influence of middle leg stepping is qualitatively different from that of forward front and backward hind leg stepping. Our results indicate that in an insect walking system individual segments differ with respect to their intersegmental influences and cannot be treated as similar within the chain of segmental walking pattern generators. Consequences for current concepts on intersegmental coordination are discussed.