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J Neurophysiol (June 15, 2005). doi:10.1152/jn.00414.2005
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00414.2005v1
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Submitted on April 22, 2005
Accepted on June 7, 2005

Effects of salicylate on plasma membrane mechanics

Sergey A. Ermilov1*, David R. Murdock1, Dania El-Daye1, William E. Brownell2, and Bahman Anvari1

1 Department of Bioengineering, Rice University, Houston, Texas, USA
2 Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine, Houston, Texas, USA

* To whom correspondence should be addressed. E-mail: ermilovs{at}rice.edu.

High concentrations of the amphipathic drug salicylate (Sal) block outer hair cell (OHC) electromotility resulting in reversible hearing loss. We have used optical tweezers to study the effects of Sal on the mechanics of the cell plasma membrane. Membrane tethers were formed from guinea pig OHCs and cultured human embryonic kidney (HEK) cells as controls. HEK cells are commonly used in functional expression studies of electromotility. Effective tether viscosity ({eta}eff), steady-state tethering force extrapolated to zero pulling rate (Fss(0)), and time constant for tether growth ({tau}tg) were estimated from the measurements of the instantaneous tethering force at different tether pulling rates. Average values of {eta}eff, Fss(0), and {tau}tg for the OHC lateral wall plasma membrane and control cell plasma membrane remained the same after Sal perfusion, which is consistent with the hypothesis that Sal induced reversible hearing loss appears to be more the result of its competition with essential anions and less the result of a change in plasma membrane mechanics.




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