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J Neurophysiol (September 14, 2005). doi:10.1152/jn.00658.2005
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Submitted on June 24, 2005
Accepted on September 7, 2005

In vitro characterization of L-type calcium channels and their contribution to firing behaviour in invertebrate respiratory neurons

J. David Spafford1, Tyler W. Dunn1, August B. Smit1, Naweed I. Syed1, and Gerald W. Zamponi1*

1 Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada

* To whom correspondence should be addressed. E-mail: zamponi{at}ucalgary.ca.

L-type calcium channel activity has been associated with a number of cytoplasmic responses, including gene transcription and activation of calcium-dependent enzymes, yet their direct contribution to the electrical activities of neurons has remained largely unexplored. Here we report the cloning and functional characterization of a molluscan L-type calcium channel homolog, LCav1, and investigate its role in coordinating neuronal firing patterns. The LCav1 channel exhibits many hallmarks of vertebrate L-type channels in that it is high voltage activated, slowly inactivating, dihydropyridine sensitive and displays calcium dependent inactivation in recording solutions with standard EGTA concentrations. We show that despite comprising less than ~20% of the total whole cell current in identified Lymnaea respiratory network neurons, the L-type channels are essential for maintaining rhythmic action potential discharges without being involved in synaptic release. Our data therefore suggest an important role of L-type calcium channels in maintaining rhythmical pattern activity underlying breathing behaviour in Lymnaea.




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