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J Neurophysiol (October 22, 2003). doi:10.1152/jn.00744.2003
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Submitted on August 1, 2003
Accepted on October 19, 2003

Median and Dorsal Raphe Neurons Are Not Electrophysiologically Identical

Sheryl G. Beck1*, Yu-Zhen Pan1, Adaure C. Akanwa1, and Lynn G. Kirby1

1 Pediatrics, University of Pennsylvania and Children's Hospital of Philadelphia, Philadelphia, PA, USA

* To whom correspondence should be addressed. E-mail: becks{at}email.chop.edu.

The dorsal (DR) and median raphe (MR) nuclei contain 5-hydroxytryptamine (serotonin, 5-HT) cell bodies that give rise to the majority of the ascending 5-HT projections to the forebrain limbic areas that control emotional behavior. In the past the electrophysiological identification of neurochemically identified 5-HT neurons has been limited. Recent technical developments have made it possible to re-examine the electrophysiological characteristics of identified 5-HT and non-5-HT containing neurons. Visualized whole cell electrophysiological techniques in combination with fluorescence immunohistochemistry for 5-HT were used. In the DR both 5-HT and non-5-HT containing neurons exhibited similar characteristics that have historically been attributed to putative 5-HT neurons. In contrast in the MR the 5-HT and non-5-HT containing neurons had very different characteristics. Interestingly, the MR 5-HT containing neurons had a shorter time constant and larger afterhyperpolarization (AHP) amplitude than DR 5-HT containing neurons. The 5-HT1A receptor-mediated response was also measured. The efficacy of the response elicited by 5-HT1A receptor-activation was greater in 5-HT containing neurons in the DR than the MR, whereas the potency was similar, implicating greater autoinhibition in the DR. Non-5-HT containing neurons in the DR were responsive to 5-HT1A receptor-activation whereas the non-5-HT containing neurons in the MR were not. These differences in the cellular characteristics and 5-HT1A receptor-mediated responses between the MR and DR neurons may be extremely important in understanding the role of these two 5-HT circuits in normal physiological processes and in the etiology and treatment of pathophysiological states.




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