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1 Neurobiology & Behavior, Cornell University, Ithaca, NY, USA
2 Neurobiology & Behavior, Cornell University, Ithaca, NY, USA; Biological Science, Columbia University, New York, NY, USA
3 Millennium Pharmaceuticals, Cambridge, MA, USA
* To whom correspondence should be addressed. E-mail: yz34{at}cornell.edu.
The transient potassium current (IA) plays an important role in shaping the firing properties of pyloric neurons in the stomatogastric ganglion (STG) of the spiny lobster, Panulirus interruptus. The shal gene encodes IA in pyloric neurons. However, when we over-expressed the lobster Shal protein by shal RNA injection into the Pyloric Dilator (PD) neuron, the increased IA had somewhat different properties from the endogenous IA. The recently cloned KChIPs (K-channel interacting proteins) can modify vertebrate Kv4 channels in cloned cell lines. When we co-expressed hKChIP1 with lobster shal in Xenopus oocytes or lobster PD neurons, they produced A-currents resembling the endogenous IA in PD neurons: compared to currents evoked by shal alone, their voltage for half inactivation was depolarized, their kinetics of inactivation were slowed, and their recovery from inactivation was accelerated. We also co-expressed shal in PD neurons with lobster frequenin, which encodes a protein belonging to the same EF-hand family of Ca2+ sensing proteins as hKChIP. Frequenin also restored most of properties of the shal-evoked currents to those of the endogenous A-currents, but the time course of recovery from inactivation was not corrected. These results suggest that lobster shal proteins normally interact with proteins in the KChIP/frequenin family to produce the transient potassium current in pyloric neurons.
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