| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Biomedical Sciences, University of Maryland Dental School, Baltimore, MD, USA; Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD, USA; Program in Neuroscience, University of Maryland, Baltimore, Baltimore, MD, USA
* To whom correspondence should be addressed. E-mail: msg001{at}dental.umaryland.edu.
The present study was designed to test the hypotheses that pain syndromes associated with specific body regions reflect unique properties of sensory neurons innervating these regions and/or unique responses of these afferents to tissue damage. Acutely dissociated adult rat DRG neurons retrogradely labeled from either the colon or the glabrous skin of the hindpaw were studied by whole-cell patch clamp recording in current clamp mode. Two populations of colonic afferent neurons were studied: pelvic afferents (arising from L6, S1 and S2 DRG = LS DRG) and hypogastric/lumbar colonic afferents (arising from T13, L1 and L2 DRG = TL DRG). Passive and active electrophysiological properties were studied before and after application prostaglandin E2 (PGE2). We observed marked differences between cutaneous and colonic sensory neurons with respect to baseline passive and active electrophysiological properties as well as both the magnitude and pattern of PGE2-induced changes in excitability, passive and active properties. There were also significant differences between TL and LS neurons with respect to baseline and PGE2-induced changes in several passive and active electrophysiological properties. Our results suggest that differences between cutaneous and colonic neurons reflect differences in pattern and/or density of ionic currents present in the plasma membrane. More interestingly, the ionic currents underlying the PGE2-induced sensitization of cutaneous neurons appeared to differ from those underlying the sensitization of colonic neurons. The implication of this observation is that it may be possible, in fact necessary, to treat pain arising from specific body regions with unique therapeutic interventions.
This article has been cited by other articles:
|
|
 |
|
  W. E. B. Sipe, S. M. Brierley, C. M. Martin, B. D. Phillis, F. B. Cruz, E. F. Grady, W. Liedtke, D. M. Cohen, S. Vanner, L. A. Blackshaw, et al. Transient receptor potential vanilloid 4 mediates protease activated receptor 2-induced sensitization of colonic afferent nerves and visceral hyperalgesia Am J Physiol Gastrointest Liver Physiol, May 1, 2008; 294(5): G1288 - G1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Dang, K. Lamb, M. Cohen, K. Bielefeldt, and G. F. Gebhart Cyclophosphamide-Induced Bladder Inflammation Sensitizes and Enhances P2X Receptor Function in Rat Bladder Sensory Neurons J Neurophysiol, January 1, 2008; 99(1): 49 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sugiura, K. Bielefeldt, and G. F. Gebhart Mouse colon sensory neurons detect extracellular acidosis via TRPV1 Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1768 - C1774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. Winnard, N. Dmitrieva, and K. J. Berkley Cross-organ interactions between reproductive, gastrointestinal, and urinary tracts: modulation by estrous stage and involvement of the hypogastric nerve Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1592 - R1601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-G. Lu, X. Zhang, and M. S. Gold Intracellular calcium regulation among subpopulations of rat dorsal root ganglion neurons J. Physiol., November 15, 2006; 577(1): 169 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ma, K. W. Greenquist, and R. H. LaMotte Inflammatory Mediators Enhance the Excitability of Chronically Compressed Dorsal Root Ganglion Neurons J Neurophysiol, April 1, 2006; 95(4): 2098 - 2107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wang, B. Tang, and R. J. Traub Differential Processing of Noxious Colonic Input by Thoracolumbar and Lumbosacral Dorsal Horn Neurons in the Rat J Neurophysiol, December 1, 2005; 94(6): 3788 - 3794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Dang, K. Bielefeldt, and G. F. Gebhart Differential Responses of Bladder Lumbosacral and Thoracolumbar Dorsal Root Ganglion Neurons to Purinergic Agonists, Protons, and Capsaicin J. Neurosci., April 13, 2005; 25(15): 3973 - 3984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sugiura, K. Dang, K. Lamb, K. Bielefeldt, and G. F. Gebhart Acid-Sensing Properties in Rat Gastric Sensory Neurons from Normal and Ulcerated Stomach J. Neurosci., March 9, 2005; 25(10): 2617 - 2627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Flake, D. B. Bonebreak, and M. S. Gold Estrogen and Inflammation Increase the Excitability of Rat Temporomandibular Joint Afferent Neurons J Neurophysiol, March 1, 2005; 93(3): 1585 - 1597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Beyak, N. Ramji, K. M. Krol, M. D. Kawaja, and S. J. Vanner Two TTX-resistant Na+ currents in mouse colonic dorsal root ganglia neurons and their role in colitis-induced hyperexcitability Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G845 - G855. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
