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1 Laboratory for Neuronal Circuit Dynamics, Brain Science Insitute, RIKEN, Wako-shi, Saitama, Japan; Department of Neuroscience and Rita Montalcini Centre for Brain Repair, University of Turin, Turin, Italy
2 Department of Neuroscience and Rita Montalcini Centre for Brain Repair, University of Turin, Turin, Italy
3 Laboratory for Neuronal Circuit Dynamics, Brain Science Insitute, RIKEN, Wako-shi, Saitama, Japan
* To whom correspondence should be addressed. E-mail: knopfel{at}brain.riken.go.jp.
Maturation of specific neuronal connections in the mature nervous system includes elimination of redundant synapses formed earlier during development. In the cerebellum of adult animals, each Purkinje cell (PC) is innervated by a single climbing fiber (CF). In early postnatal development each PC is innervated by multiple CFs and elimination of synapses formed by supernumerary CFs occurs until monoinnervation is established at around post-natal day 20 (P20) in mice. It is not clear whether multiple CFs, or only a single CF, translocate from the cell body of immature PCs to the developing dendrite and, in case several CFs translocate, if they share or segregate their innervation fields. To localize CF innervation fields, we imaged changes in postsynaptic sodium concentration resulting from CF-mediated postsynaptic currents. We found that more than one CF translocates from an innervation field on the cell body of the PC to the developing dendrite and that these CFs share rather than segregate their innervation fields. We concluded that both the soma and the proximal dendrite of the PC are territories of competition for the developing CFs and that the overlapping of their termination fields may be the prerequisite for a local process of elimination of all but one CF as previously demonstrated in the developing neuromuscular junction.
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