The nucleus of the tractus solitarius (NTS) plays an important role in the control of several autonomic reflex functions and has glutamate and GABA as main neurotransmitters. In this work we used the patch clamp technique in transverse slice preparations from rats to investigate whether the glycine binding site of the NMDA receptor is saturated or not in neurons of the subpostremal NTS. Except at hyperpolarized voltages and close to the reversal potential, glycine potentiated the NMDA responses in a concentration-dependent manner. The total charge transferred by glutamatergic currents was enhanced by glycine (500 µM) (from 28 ± 13 pC to 42 ± 18 pC at + 50 mV, n = 7, p < 0.05). Glycine increased the conductance of the postsynaptic membrane, without altering its reversal potential, both in the presence (from 2.4 ± 0.06 nS to 3.4 ± 0.09 nS; n = 7) and absence (from 3.1 ± 0.06 nS to 4.4 ± 0.10 nS; n = 8) of Mg²⁺ in the bathing solution. D-serine, in the presence of strychnine, also increased the amplitude of the NMDA component (by 68 ± 19 %, p < 0.05, n = 5). The membrane potential was hyperpolarized (16 ± 6 mV, n = 8) by glycine suggesting the presence of inhibitory glycinergic receptors. Our results indicate that the glycine site of the NMDA receptor in neurons of the subpostremal NTS is not saturated and that glycine may act as a modulator of the NMDA transmission in this nucleus.