| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Applied and Engineering Physics, Cornell University, Ithaca, NY, USA
* To whom correspondence should be addressed. E-mail: www2{at}cornell.edu.
Although fluorescence microscopy has proven to be one of the most powerful tools in biology, its application to the intact animal has been limited to imaging several hundred microns below the surface. The rest of the animal has eluded investigation at the microscopic level without excising tissue or performing extensive surgery. However, the ability to image with subcellular resolution in the intact animal enables a contextual setting that may be critical for understanding proper function. Clinical applications such as disease diagnosis and optical biopsy may benefit from minimally invasive in vivo approaches. Gradient index (GRIN) lenses with needle-like dimensions can transfer high-quality images many centimeters from the object plane. Here, we show that multiphoton microscopy through GRIN lenses enables minimally invasive, subcellular resolution several millimeters in the anesthetized, intact animal, and we present in vivo images of cortical layer V and hippocampus in anesthetized Thy1-YFP line H mouse. Microangiographies from deep capillaries and blood vessels containing fluorescein-dextran and quantum dot labeled serum in wild-type mouse brain are also demonstrated.
This article has been cited by other articles:
|
|
 |
|
  T. H. Chia and M. J. Levene Microprisms for In Vivo Multilayer Cortical Imaging J Neurophysiol, August 1, 2009; 102(2): 1310 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nimmerjahn Astrocytes going live: advances and challenges J. Physiol., April 15, 2009; 587(8): 1639 - 1647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kuga, T. Hirata, I. Sakai, Y. Tanikawa, H. Y. Chiou, T. Kitanishi, N. Matsuki, and Y. Ikegaya Rapid and local autoregulation of cerebrovascular blood flow: a deep-brain imaging study in the mouse J. Physiol., February 15, 2009; 587(4): 745 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Deisseroth, G. Feng, A. K. Majewska, G. Miesenbock, A. Ting, and M. J. Schnitzer Next-Generation Optical Technologies for Illuminating Genetically Targeted Brain Circuits J. Neurosci., October 11, 2006; 26(41): 10380 - 10386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yao, D. R. Larson, H. D. Vishwasrao, W. R. Zipfel, and W. W. Webb Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution PNAS, October 4, 2005; 102(40): 14284 - 14289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zenisek Vesicle reuse revisited PNAS, May 24, 2005; 102(21): 7407 - 7408. [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Stutzmann and I. Parker Dynamic Multiphoton Imaging: A Live View from Cells to Systems Physiology, February 1, 2005; 20(1): 15 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rubart Two-Photon Microscopy of Cells and Tissue Circ. Res., December 10, 2004; 95(12): 1154 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer In Vivo Mammalian Brain Imaging Using One- and Two-Photon Fluorescence Microendoscopy J Neurophysiol, November 1, 2004; 92(5): 3121 - 3133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Brecht, M. S. Fee, O. Garaschuk, F. Helmchen, T. W. Margrie, K. Svoboda, and P. Osten Novel Approaches to Monitor and Manipulate Single Neurons In Vivo J. Neurosci., October 20, 2004; 24(42): 9223 - 9227. [Full Text] [PDF]
|
|
|
|
 |
|
 S. X. Lin and F. R. Maxfield Fluorescence imaging in living animals. Focus on "Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy" Am J Physiol Cell Physiol, August 1, 2004; 287(2): C257 - C259. [Full Text] [PDF]
|
|
|
|
|
|
A. Mizrahi, J. C. Crowley, E. Shtoyerman, and L. C. Katz High-Resolution In Vivo Imaging of Hippocampal Dendrites and Spines J. Neurosci., March 31, 2004; 24(13): 3147 - 3151. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
