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1 Neurology , Yale University School of Medicine, New Haven, Connecticut, United States; Center for Neuroscience and Regeneration Research, Yale University School of Medicine, New Haven, Connecticut, United States; Rehabilitation Research Center, VA Connecticut Healthcare System, West Haven, Connecticut, United States
2 West Haven, Connecticut, United States; Neurology , Yale University School of Medicine, New Haven, Connecticut, United States; Dept. Neurology Yale Univ Sch Med, West Haven, Connecticut, 06516, United States; Center for Neuroscience and Regeneration Research, Yale University School of Medicine, New Haven, Connecticut, United States; Rehabilitation Research Center, VA Connecticut Healthcare System, West Haven, Connecticut, United States
3 Neurology, Yale University School of Medicine, LCI 707, New Haven, Connecticut, 06510, United States; Center for Neuroscience and Regeneration Research, Yale University School of Medicine, New Haven, Connecticut, United States; Rehabilitation Research Center, VA Connecticut Healthcare System, West Haven, Connecticut, United States
* To whom correspondence should be addressed. E-mail: stephen.waxman{at}yale.edu.
Nociceptive dorsal root ganglion (DRG) neurons can be a classified into non-peptidergic IB4+ and peptidergic IB4- subtypes, which terminate in different layers in dorsal horn and transmit pain along different ascending pathways, and display different firing properties. Voltage-gated, tetrodotoxin-resistant (TTX-R) Nav1.8 channels are expressed in both IB4+ and IB4- cells and produce most of the current underlying the depolarizing phase of action potential (AP). Slow inactivation of TTX-R channels has been shown to regulate repetitive DRG neuron firing behavior. We show in this study that use-dependent reduction of Nav1.8 current in IB4+ neurons is significantly stronger than in IB4- neurons, although voltage-dependence of activation and steady-state inactivation are not different. The time constant for entry of Nav1.8 into slow inactivation in IB4+ neurons is significantly faster and more Nav1.8 enter the slow inactivation state than in IB4- neurons. In addition, recovery from slow inactivation of Nav1.8 in IB4+ neurons is slower than in IB4- neurons. Using current-clamp recording, we demonstrate a significantly higher current threshold for generation of APs and a longer latency to onset of firing in IB4+, compared with IB4- neurons. In response to a ramp stimulus, IB4+ neurons produce fewer APs and display stronger adaptation, with faster decline of AP peak than IB4- neurons. Our data suggest that differential use-dependent reduction of Nav1.8 current in these two DRG subpopulations, which results from their different rate of entry into and recovery from the slow inactivation state, contributes to functional differences between these two neuronal populations.
This article has been cited by other articles:
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  A. Hudmon, J.-S. Choi, L. Tyrrell, J. A. Black, A. M. Rush, S. G. Waxman, and S. D. Dib-Hajj Phosphorylation of Sodium Channel Nav1.8 by p38 Mitogen-Activated Protein Kinase Increases Current Density in Dorsal Root Ganglion Neurons J. Neurosci., March 19, 2008; 28(12): 3190 - 3201. [Abstract] [Full Text] [PDF]
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 A. M. Rush, T. R. Cummins, and S. G. Waxman Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons J. Physiol., February 15, 2007; 579(1): 1 - 14. [Abstract] [Full Text] [PDF]
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