JN Miami Valley Hospital
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Neurophysiol (March 31, 2004). doi:10.1152/jn.01099.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
92/6/3433    most recent
01099.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, M.
Right arrow Articles by Nurse, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, M.
Right arrow Articles by Nurse, C. A.
Submitted on November 13, 2003
Accepted on March 23, 2004

CO2/pH chemosensory signalling in co-cultures of rat carotid body receptors and petrosal neurons: role of ATP and ACh

Min Zhang and Colin A. Nurse*

* To whom correspondence should be addressed. E-mail: nursec{at}mcmaster.ca.

The neurotransmitter mechanisms that process acid hypercapnia in the mammalian carotid body (CB) are poorly understood. Using a co-culture model, containing rat CB chemoreceptor (type 1 cell) clusters and petrosal neurons (PN), we tested the hypothesis that co-released ACh and ATP was an important mechanism. Sensory transmission from type I clusters to PN in co-culture occurred at chemical synapses via co-release of ATP and ACh since: (i) isohydric hypercapnia depolarized and/or increased firing in co-cultured PN, but not in PN cultured alone; (ii) PN chemoexcitatory responses were inhibited by decreasing the extracellular Ca2+: Mg2+ ratio; (iii) partial inhibition of these responses occurred during separate perfusion of cholinergic (hexamethonium or mecamylamine) and P2X (suramin) receptor blockers, though inhibition was often complete with both blockers present; (iv) rapid chemoexcitatory responses to hypercapnia were inhibited by acetazolamide (10 µM), an inhibitor of carbonic anhydrase, localized in type I cells. Acidosis (pH = 7.0, 7.2) enhanced the ATP-induced whole-cell current in functional PN, relative to that at physiologic pH (7.4), suggesting that increased sensitivity of postsynaptic P2X receptors may contribute to acid chemotransmission. Type I cells in CB tissue sections expressed vesicular acetylcholine transporter (VAChT), a cholinergic marker, as revealed by confocal immunofluorescence. Thus, co-release of ACh and ATP is an important neurotransmitter mechanism for processing isohydric and acidic hypercapnia in the rat carotid body.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
A. V. Gourine, N. Dale, A. Korsak, E. Llaudet, F. Tian, R. Huckstepp, and K. M. Spyer
Release of ATP and glutamate in the nucleus tractus solitarii mediate pulmonary stretch receptor (Breuer-Hering) reflex pathway
J. Physiol., August 15, 2008; 586(16): 3963 - 3978.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. Buttigieg, S. Brown, M. Zhang, M. Lowe, A. C. Holloway, and C. A. Nurse
Chronic nicotine in utero selectively suppresses hypoxic sensitivity in neonatal rat adrenal chromaffin cells
FASEB J, May 1, 2008; 22(5): 1317 - 1326.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Yamauchi, J. Dostal, and K. P. Strohl
Acetazolamide protects against posthypoxic unstable breathing in the C57BL/6J mouse
J Appl Physiol, October 1, 2007; 103(4): 1263 - 1268.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. A. Braga, R. N. Soriano, A. L. Braccialli, P. M. de Paula, L. G. H. Bonagamba, J. F. R. Paton, and B. H. Machado
Involvement of L-glutamate and ATP in the neurotransmission of the sympathoexcitatory component of the chemoreflex in the commissural nucleus tractus solitarii of awake rats and in the working heart-brainstem preparation
J. Physiol., June 15, 2007; 581(3): 1129 - 1145.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Burnstock
Physiology and Pathophysiology of Purinergic Neurotransmission
Physiol Rev, April 1, 2007; 87(2): 659 - 797.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Zhang, J. Buttigieg, and C. A. Nurse
Neurotransmitter mechanisms mediating low-glucose signalling in cocultures and fresh tissue slices of rat carotid body
J. Physiol., February 1, 2007; 578(3): 735 - 750.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. A. Campanucci, M. Zhang, C. Vollmer, and C. A. Nurse
Expression of Multiple P2X Receptors by Glossopharyngeal Neurons Projecting to Rat Carotid Body O2-Chemoreceptors: Role in Nitric Oxide-Mediated Efferent Inhibition.
J. Neurosci., September 13, 2006; 26(37): 9482 - 9493.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
B. Vulesevic, B. McNeill, and S. F. Perry
Chemoreceptor plasticity and respiratory acclimation in the zebrafish Danio rerio
J. Exp. Biol., April 1, 2006; 209(7): 1261 - 1273.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
N. R Prabhakar
O2 sensing at the mammalian carotid body: why multiple O2 sensors and multiple transmitters?
Exp Physiol, January 1, 2006; 91(1): 17 - 23.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. V Gourine
On the peripheral and central chemoreception and control of breathing: an emerging role of ATP
J. Physiol., November 1, 2005; 568(3): 715 - 724.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2004 by the The American Physiological Society.