JN Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Neurophysiol (April 2, 2003). doi:10.1152/jn.01174.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
90/2/1257    most recent
01174.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Sun, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Sun, D.
Submitted on December 30, 2002
Accepted on March 31, 2003

GABA-mediated trophic effect on oligodendrocytes requires Na-K-2Cl cotransport activity

Hao Wang1, Yiping Yan1, Douglas B. Kintner1, Christian Lytle2, and Dandan Sun3*

1 Neurosurgery, University of Wisconsin Medical School, Madison, WI, USA
2 Division of Biomedical Science, University of California-Riverside, Riverside, CA, USA
3 Neurosurgery, University of Wisconsin Medical School, Madison, WI, USA; Physiology, University of Wisconsin Medical School, Madison, WI, USA

* To whom correspondence should be addressed. E-mail: sun{at}neurosurg.wisc.edu.

The Na-K-2Cl cotransporter isoform1 (NKCC1) is present in many animal cells where it plays prominent roles in regulating cell volume and maintaining intracellular Cl- concentration ([Cl-]i) above electrochemical equilibrium. We show here that NKCC1 is present and active in cultured oligodendrocytes. Expression of NKCC1 in the rat spinal cord increased during development from postnatal day 6 through 21 in parallel with that of myelin basic protein. In cultured oligodendrocytes, 39% of the total K+(86Rb+) influx represented NKCC1 activity. Activation of GABAA receptors with muscimol produced a reduction in intracellular Cl- content, cell shrinkage, and a stimulation of NKCC1 activity. Muscimol also triggered an increase in intracellular Ca2+; this increase depended on NKCC1 activity. Survival of oligodendrocytes following withdrawal of growth factors was enhanced by muscimol; this effect also required NKCC1 activity. Our results suggest that NKCC1 functions in oligodendrocytes to maintain [Cl-]i above electrochemical equilibrium, and that NKCC1 is required for GABAergic trophic effects.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
H. I. Rocha-Gonzalez, S. Mao, and F. J. Alvarez-Leefmans
Na+,K+,2Cl- Cotransport and Intracellular Chloride Regulation in Rat Primary Sensory Neurons: Thermodynamic and Kinetic Aspects
J Neurophysiol, July 1, 2008; 100(1): 169 - 184.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. F. Pedersen, M. E. O'Donnell, S. E. Anderson, and P. M. Cala
Physiology and pathophysiology of Na+/H+ exchange and Na+-K+-2Cl- cotransport in the heart, brain, and blood
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R1 - R25.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. Wouters, A. D. Laet, L. V. Donck, E. Delpire, P.-P. van Bogaert, J.-P. Timmermans, A. de Kerchove d'Exaerde, K. Smans, and J.-M. Vanderwinden
Subtractive hybridization unravels a role for the ion cotransporter NKCC1 in the murine intestinal pacemaker
Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1219 - G1227.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
B. Lenart, D. B. Kintner, G. E. Shull, and D. Sun
Na-K-Cl Cotransporter-Mediated Intracellular Na+ Accumulation Affects Ca2+ Signaling in Astrocytes in an In Vitro Ischemic Model
J. Neurosci., October 27, 2004; 24(43): 9585 - 9597.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2003 by the The American Physiological Society.