JN AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Neurophysiol (March 14, 2007). doi:10.1152/jn.01178.2006
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Figure and Movies
Right arrow All Versions of this Article:
97/5/3751    most recent
01178.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berger, T.
Right arrow Articles by Petersen, C. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berger, T.
Right arrow Articles by Petersen, C. C.
Submitted on November 6, 2006
Accepted on March 10, 2007

Combined voltage and calcium epifluorescence imaging in vitro and in vivo reveals subthreshold and suprathreshold dynamics of mouse barrel cortex

Thomas Berger1, Aren Jacobus Borgdorff2, Sylvain Crochet2, Florian Neubauer1, Sandrine Lefort2, Bruno Fauvet2, Isabelle Ferezou2, Alan Carleton3, Hans-R. Rudolf Luscher1, and Carl CH Petersen2*

1 University of Bern, Institute of Physiology, Bern, Switzerland
2 Laboratory of Sensory Processing, Brain and Mind Institute, Lausanne, Switzerland
3 Flavor Perception Group, Brain and Mind Institute, Lausanne, Switzerland

* To whom correspondence should be addressed. E-mail: carl.petersen{at}epfl.ch.

Cortical dynamics can be imaged at high spatiotemporal resolution with voltage-sensitive dyes and calcium-sensitive dyes. We combined these two imaging techniques using epifluorescence optics together with whole-cell recordings to measure the spatiotemporal dynamics of activity in the mouse somatosensory barrel cortex in vitro and in the supragranular layers in vivo. The two optical signals reported distinct aspects of cortical function. Voltage-sensitive dye fluorescence varied linearly with membrane potential and was dominated by subthreshold postsynaptic potentials, whereas the calcium-sensitive dye signal predominantly reflected local action potential firing. Combining voltage-sensitive dyes and calcium-sensitive dyes allowed us to monitor the synaptic drive and the spiking activity of a given area at the same time in the same preparation. The spatial extent of the two dye signals was different, with voltage-sensitive dye signals spreading further than calcium-sensitive dye signals, reflecting broad subthreshold and narrow suprathreshold receptive fields. Importantly, the signals from the dyes were differentially affected by pharmacological manipulations, stimulation strength and depth of anesthesia. Combined voltage-sensitive dye and calcium-sensitive dye measurements can therefore be used to specify the temporal and spatial relationships between subthreshold and suprathreshold activity of the neocortex.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
I. R. Winship and T. H. Murphy
In Vivo Calcium Imaging Reveals Functional Rewiring of Single Somatosensory Neurons after Stroke
J. Neurosci., June 25, 2008; 28(26): 6592 - 6606.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Kuhn, W. Denk, and R. M. Bruno
In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 and 2 during wakefulness
PNAS, May 27, 2008; 105(21): 7588 - 7593.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
C. G. Schipke, B. Haas, and H. Kettenmann
Astrocytes Discriminate and Selectively Respond to the Activity of a Subpopulation of Neurons within the Barrel Cortex
Cereb Cortex, March 4, 2008; (2008) bhn009v1.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2007 by the The American Physiological Society.