JN AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Neurophysiol (January 25, 2006). doi:10.1152/jn.01352.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
95/5/2878    most recent
01352.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by King, J. T.
Right arrow Articles by McCobb, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by King, J. T.
Right arrow Articles by McCobb, D. P.
Submitted on December 22, 2005
Accepted on January 24, 2006

{beta}2 and {beta}4 Subunits of BK Channels Confer Differential Sensitivity to Acute Modulation by Steroid Hormones

Jonathan T. King1, Peter V. Lovell1, Mark Rishniw2, Michael I. Kotlikoff2, Mary Lou Zeeman3, and David P. McCobb1*

1 Neurobiology and Behavior, Cornell University, Ithaca, NY, USA
2 Biomedical Sciences, Cornell University, Ithaca, NY, USA
3 Applied Mathematics, University of Texas at San Antonio, San Antonio, TX, USA

* To whom correspondence should be addressed. E-mail: dpm9{at}cornell.edu.

Membrane-associated receptors for rapid, steroidal neuromodulation remain elusive. Estradiol has been reported to facilitate activation of voltage- and calcium-dependent "BK" potassium channels encoded by Slo, if associated with {beta}1 subunits. We demonstrate here that 1) multiple members of the {beta} family confer sensitivity to multiple steroids on BK channels, 2) that {beta} subunits differentiate between steroids, and 3) that different {beta}s have distinct relative preferences for particular steroids. Expressed in HEK 293 cells, inside-out patches with channels composed of Slo-{alpha} alone showed no steroid sensitivity. Cells expressing {alpha}{beta}4 exhibited potent, rapid, reversible, and dose-dependent potentiation by corticosterone (CORT, a glucocorticoid), and were potentiated to a lesser degree by other sex and stress steroids. In contrast, {alpha}{beta}2 channels were potentiated more strongly by dehydroepiandrosterone (DHEA, an enigmatic, stress-related, adrenal androgen), and to a lesser extent by CORT, estradiol, testosterone, and DHEA-S. Cholesterol had no effect on any BK channel compositions tested. Conductance-voltage plots of channels composed of a plus {beta}2 or {beta}4 subunits were shifted in the negative direction by steroids, indicating greater activation at negative voltages. Thus our results argue that the variety of Slo-{beta} subunit coexpression patterns occurring in vivo expands the repertoire of Slo channel gating in yet another dimension not fully appreciated, rendering BK gating responsive to dynamic fluctuations in a multiple of steroid hormones.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Tong and R. K. Duncan
Tamoxifen inhibits BK channels in chick cochlea without alterations in voltage-dependent activation
Am J Physiol Cell Physiol, July 1, 2009; 297(1): C75 - C85.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
V. G. Romanenko, K. S. Roser, J. E. Melvin, and T. Begenisich
The role of cell cholesterol and the cytoskeleton in the interaction between IK1 and maxi-K channels
Am J Physiol Cell Physiol, April 1, 2009; 296(4): C878 - C888.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
S. Hou, S. H. Heinemann, and T. Hoshi
Modulation of BKCa Channel Gating by Endogenous Signaling Molecules
Physiology, February 1, 2009; 24(1): 26 - 35.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. N. Bukiya, J. McMillan, A. L. Parrill, and A. M. Dopico
Structural determinants of monohydroxylated bile acids to activate {beta}1 subunit-containing BK channels
J. Lipid Res., November 1, 2008; 49(11): 2441 - 2451.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. J. Brunton, M. Sausbier, G. Wietzorrek, U. Sausbier, H.-G. Knaus, J. A. Russell, P. Ruth, and M. J. Shipston
Hypothalamic-Pituitary-Adrenal Axis Hyporesponsiveness to Restraint Stress in Mice Deficient for Large-Conductance Calcium- and Voltage-Activated Potassium (BK) Channels
Endocrinology, November 1, 2007; 148(11): 5496 - 5506.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. P. Torres, F. J. Morera, I. Carvacho, and R. Latorre
A Marriage of Convenience: beta-Subunits and Voltage-dependent K+ Channels
J. Biol. Chem., August 24, 2007; 282(34): 24485 - 24489.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. N. Bukiya, J. Liu, L. Toro, and A. M. Dopico
beta1 (KCNMB1) Subunits Mediate Lithocholate Activation of Large-Conductance Ca2+-Activated K+ Channels and Dilation in Small, Resistance-Size Arteries
Mol. Pharmacol., August 1, 2007; 72(2): 359 - 369.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. Coiret, A.-S. Borowiec, P. Mariot, H. Ouadid-Ahidouch, and F. Matifat
The Antiestrogen Tamoxifen Activates BK Channels and Stimulates Proliferation of MCF-7 Breast Cancer Cells
Mol. Pharmacol., March 1, 2007; 71(3): 843 - 851.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
P. Chameau, Y. Qin, S. Spijker, G. Smit, and M. Joels
Glucocorticoids Specifically Enhance L-Type Calcium Current Amplitude and Affect Calcium Channel Subunit Expression in the Mouse Hippocampus
J Neurophysiol, January 1, 2007; 97(1): 5 - 14.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2006 by the The American Physiological Society.