| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Internal Medicine, Section of Human Physiology, University of Perugia, I-06126 Perugia 2 Department of Biotechnology and Biosciences, University of Milano-Bicocca, I-20126 Milan, Italy
Submitted 4 February 2003; accepted in final form 12 May 2003
Transcripts encoding ERG potassium channels are expressed by most neurons of the CNS. By patch-clamp whole cell recording from Purkinje neurons in slices of young (5–9 days old) mouse cerebellum we have been able to isolate a tail current [IK(ERG)] with the same characteristics as previously described for ERG channels. In zero external Ca2+ and high K+ (40 mM) the V1/2 of activation was –50.7 mV, the V1/2 of inactivation was –70.6 mV, and the deactivation rate was double exponential and voltage dependent. IK(ERG) was 93.0% blocked by WAY-123,398 (1 µM) and 78.2% by haloperidol (2 µM). The role of IK(ERG) on evoked firing was studied in adult mice, where WAY-123,398 application decreased the first spike latency, increased the firing frequency, and suppressed the frequency adaptation. However, the shape of individual action potentials was not affected. Stimulation of presynaptic climbing fibers evoked the Purkinje neuron "complex spike," composed of an initial spike and several spikelets. IK(ERG) block caused an increase of the number of spikelets of the "complex spike." These data show, for the first time, an IK(ERG) in a neuron of the CNS, the cerebellar Purkinje neuron, and indicate that such a current is involved in the control of membrane excitability, firing frequency adaptation, and in determining the effects of the climbing fiber synapse.
This article has been cited by other articles:
|
|
 |
|
  R. M. Hardman and I. D. Forsythe Ether-à-go-go-related gene K+ channels contribute to threshold excitability of mouse auditory brainstem neurons J. Physiol., June 1, 2009; 587(11): 2487 - 2497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hagendorf, D. Fluegge, C. Engelhardt, and M. Spehr Homeostatic Control of Sensory Output in Basal Vomeronasal Neurons: Activity-Dependent Expression of Ether-a-Go-Go-Related Gene Potassium Channels J. Neurosci., January 7, 2009; 29(1): 206 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pessia, I. Servettini, R. Panichi, L. Guasti, S. Grassi, A. Arcangeli, E. Wanke, and V. E. Pettorossi ERG voltage-gated K+ channels regulate excitability and discharge dynamics of the medial vestibular nucleus neurones J. Physiol., October 15, 2008; 586(20): 4877 - 4890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Fernandez, J. Sargent, F. B. Sachse, and M. C. Sanguinetti Structural Basis for Ether-a-go-go-Related Gene K+ Channel Subtype-Dependent Activation by Niflumic Acid Mol. Pharmacol., April 1, 2008; 73(4): 1159 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Canavier, S. A. Oprisan, J. C. Callaway, H. Ji, and P. D. Shepard Computational Model Predicts a Role for ERG Current in Repolarizing Plateau Potentials in Dopamine Neurons: Implications for Modulation of Neuronal Activity J Neurophysiol, November 1, 2007; 98(5): 3006 - 3022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. Shepard, C. C. Canavier, and E. S. Levitan Ether-a-go-go Related Gene Potassium Channels: What's All the Buzz About? Schizophr Bull, November 1, 2007; 33(6): 1263 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Furlan, G. Taccola, M. Grandolfo, L. Guasti, A. Arcangeli, A. Nistri, and L. Ballerini ERG Conductance Expression Modulates the Excitability of Ventral Horn GABAergic Interneurons That Control Rhythmic Oscillations in the Developing Mouse Spinal Cord J. Neurosci., January 24, 2007; 27(4): 919 - 928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Restano-Cassulini, Y. V. Korolkova, S. Diochot, G. Gurrola, L. Guasti, L. D. Possani, M. Lazdunski, E. V. Grishin, A. Arcangeli, and E. Wanke Species Diversity and Peptide Toxins Blocking Selectivity of Ether-a-go-go-Related Gene Subfamily K+ Channels in the Central Nervous System Mol. Pharmacol., May 1, 2006; 69(5): 1673 - 1683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Miranda, T. Giraldez, P. de la Pena, D. G Manso, C. Alonso-Ron, D. Gomez-Varela, P. Dominguez, and F. Barros Specificity of TRH receptor coupling to G-proteins for regulation of ERG K+ channels in GH3 rat anterior pituitary cells J. Physiol., August 1, 2005; 566(3): 717 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cherubini, G. Hofmann, S. Pillozzi, L. Guasti, O. Crociani, E. Cilia, P. Di Stefano, S. Degani, M. Balzi, M. Olivotto, et al. Human ether-a-go-go-related Gene 1 Channels Are Physically Linked to {beta}1 Integrins and Modulate Adhesion-dependent Signaling Mol. Biol. Cell, June 1, 2005; 16(6): 2972 - 2983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sturm, S. Wimmers, J. R Schwarz, and C. K Bauer Extracellular potassium effects are conserved within the rat erg K+ channel family J. Physiol., April 15, 2005; 564(2): 329 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Hirdes, M. Schweizer, K. S Schuricht, S. S Guddat, I. Wulfsen, C. K Bauer, and J. R Schwarz Fast erg K+ currents in rat embryonic serotonergic neurones J. Physiol., April 1, 2005; 564(1): 33 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Hirdes, L. F. Horowitz, and B. Hille Muscarinic modulation of erg potassium current J. Physiol., August 15, 2004; 559(1): 67 - 84. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
