HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 90/6/3764    most recent 00449.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by André, S. Articles by Scamps, F. Search for Related Content PubMed PubMed Citation Articles by André, S. Articles by Scamps, F.

Axotomy-Induced Expression of Calcium-Activated Chloride Current in Subpopulations of Mouse Dorsal Root Ganglion Neurons

Institut National de la Santé et de la Recherche Médicale U-583, Université Montpellier II, 34095, Montpellier Cedex 5, France

Submitted 9 May 2003; accepted in final form 10 August 2003

Whole cell patch-clamp recordings of calcium-activated chloride current [I_Cl(Ca)] were made from adult sensory neurons of naive and axotomized mouse L₄–L₆ lumbar dorsal root ganglia after 1 day of culture in vitro. A basal I_Cl(Ca) was specifically expressed in a subset of naive medium-diameter neurons (30–40 µm). Prior nerve injury, induced by sciatic nerve transection 5 days before experiments, increased both I_Cl(Ca) amplitude and its expression in medium-diameter neurons. Moreover, nerve injury also induced I_Cl(Ca) expression in a new subpopulation of neurons, the large-diameter neurons (40–50 µm). Small-diameter neurons (inferior to 30 µm) never expressed I_Cl(Ca). Regulated I_Cl(Ca) expression was strongly correlated with injury-induced regenerative growth of sensory neurons in vitro and nerve regeneration in vivo. Cell culture on a substrate not permissive for growth, D,L-polyornithine, prevented both elongation growth and I_Cl(Ca) expression in axotomized neurons. Regenerative growth and the induction of I_Cl(Ca) expression take place 2 days after injury, peak after 5 days of conditioning in vivo, slowly declining thereafter to control values. The selective expression of I_Cl(Ca) within medium- and large-diameter neurons conditioned for rapid, efficient growth suggests that these channels play a specific role in postinjury behavior of sensory neuron subpopulations such as neuropathic pain and/or axonal regeneration.

This article has been cited by other articles:

				A. D. McClellan, M. O. Kovalenko, J. A. Benes, and D. J. Schulz Spinal Cord Injury Induces Changes in Electrophysiological Properties and Ion Channel Expression of Reticulospinal Neurons in Larval Lamprey J. Neurosci., January 16, 2008; 28(3): 650 - 659. [Abstract] [Full Text] [PDF]

				S. Pieraut, V. Laurent-Matha, C. Sar, T. Hubert, I. Mechaly, C. Hilaire, M. Mersel, E. Delpire, J. Valmier, and F. Scamps NKCC1 Phosphorylation Stimulates Neurite Growth of Injured Adult Sensory Neurons J. Neurosci., June 20, 2007; 27(25): 6751 - 6759. [Abstract] [Full Text] [PDF]

				O. Sacchi, M. L. Rossi, R. Canella, and R. Fesce Synaptic and Somatic Effects of Axotomy in the Intact, Innervated Rat Sympathetic Neuron J Neurophysiol, May 1, 2006; 95(5): 2832 - 2844. [Abstract] [Full Text] [PDF]

				A.-S. Dubreuil, H. Boukhaddaoui, G. Desmadryl, C. Martinez-Salgado, R. Moshourab, G. R. Lewin, P. Carroll, J. Valmier, and F. Scamps Role of T-Type Calcium Current in Identified D-Hair Mechanoreceptor Neurons Studied In Vitro J. Neurosci., September 29, 2004; 24(39): 8480 - 8484. [Abstract] [Full Text] [PDF]

				E. J. Oh and D. Weinreich Bradykinin decreases K+ and increases Cl- conductances in vagal afferent neurones of the guinea pig J. Physiol., July 15, 2004; 558(2): 513 - 526. [Abstract] [Full Text] [PDF]