Precise control of synaptic strength is critical for maintaining accurate network activity and normal brain functions. Several major brain diseases are related to synaptic alterations in the adult brain. Detailed descriptions of the normal physiological properties of adult synapses are scarce, mainly because of the difficulties in performing whole cell patch clamp recording in brain slices from adult animals. Here we present the portrait of excitatory synapses and intrinsic properties of medium spiny neurons (MSN) of the nucleus accumbens (NAc), a central structure of the mesocorticolimbic system, from youth (P14) to adulthood (P120). We found that intrinsic neuronal excitability decreased over development. This was mainly due to an enhancement of potassium conductance and the consequent reduction in membrane resistance. The ratio between paired-pulse synaptic responses was similar in juvenile, adolescent and adult MSN, suggesting that the probability of neurotransmitter release was unaltered. AMPA receptors-mediated (AMPAR) excitatory post-synaptic currents (EPSC) decayed slower in adult MSN. In contrast, the kinetic properties and the subunit composition of NMDAR-mediated (NMDAR) EPSC in the NAc were conserved from youth to adulthood. Changes in synaptic strength were estimated from the ratio of AMPAR to NMDAR evoked and spontaneous EPSC (AMPAR/NMDAR ratio). Although both AMPAR and NMDAR EPSC decreased over development, there was an increase of the AMPAR/NMDAR ratio that was linked to changes in NMDAR EPSC. Furthermore, the distribution of AMPAR/NMDAR ratio was more heterogeneous in MSN from adults, suggesting that synaptic strength is continuously refined during life.