| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
INNOVATIVE METHODOLOGY
Center for Molecular and Behavioral Neuroscience, Rutgers, The State University of New Jersey, Newark, New Jersey 07102
Submitted 8 December 2003; accepted in final form 6 February 2004
Most neuronal interactions in the cortex occur within local circuits. Because principal cells and GABAergic interneurons contribute differently to cortical operations, their experimental identification and separation is of utmost important. We used 64-site two-dimensional silicon probes for high-density recording of local neurons in layer 5 of the somatosensory and prefrontal cortices of the rat. Multiple-site monitoring of units allowed for the determination of their two-dimensional spatial position in the brain. Of the 
60,000 cell pairs recorded, 0.2% showed robust short-term interactions. Units with significant, short-latency (<3 ms) peaks following their action potentials in their cross-correlograms were characterized as putative excitatory (pyramidal) cells. Units with significant suppression of spiking of their partners were regarded as putative GABAergic interneurons. A portion of the putative interneurons was reciprocally connected with pyramidal cells. Neurons physiologically identified as inhibitory and excitatory cells were used as templates for classification of all recorded neurons. Of the several parameters tested, the duration of the unfiltered (1 Hz to 5 kHz) spike provided the most reliable clustering of the population. High-density parallel recordings of neuronal activity, determination of their physical location and their classification into pyramidal and interneuron classes provide the necessary tools for local circuit analysis.
This article has been cited by other articles:
|
|
 |
|
  A. P. Weible, D. C. Rowland, R. Pang, and C. Kentros Neural Correlates of Novel Object and Novel Location Recognition Behavior in the Mouse Anterior Cingulate Cortex J Neurophysiol, October 1, 2009; 102(4): 2055 - 2068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-h. Liu, P. Li, Y.-t. Li, Y. J. Sun, Y. Yanagawa, K. Obata, L. I. Zhang, and H. W. Tao Visual Receptive Field Structure of Cortical Inhibitory Neurons Revealed by Two-Photon Imaging Guided Recording J. Neurosci., August 26, 2009; 29(34): 10520 - 10532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ostojic, N. Brunel, and V. Hakim How Connectivity, Background Activity, and Synaptic Properties Shape the Cross-Correlation between Spike Trains J. Neurosci., August 19, 2009; 29(33): 10234 - 10253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hartwich, T. Pollak, and T. Klausberger Distinct Firing Patterns of Identified Basket and Dendrite-Targeting Interneurons in the Prefrontal Cortex during Hippocampal Theta and Local Spindle Oscillations J. Neurosci., July 29, 2009; 29(30): 9563 - 9574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Du, I. H. Riedel-Kruse, J. C. Nawroth, M. L. Roukes, G. Laurent, and S. C. Masmanidis High-Resolution Three-Dimensional Extracellular Recording of Neuronal Activity With Microfabricated Electrode Arrays J Neurophysiol, March 1, 2009; 101(3): 1671 - 1678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Montgomery, M. I. Betancur, and G. Buzsaki Behavior-Dependent Coordination of Multiple Theta Dipoles in the Hippocampus J. Neurosci., February 4, 2009; 29(5): 1381 - 1394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Cohen, P. Pouget, R. P. Heitz, G. F. Woodman, and J. D. Schall Biophysical Support for Functionally Distinct Cell Types in the Frontal Eye Field J Neurophysiol, February 1, 2009; 101(2): 912 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Issa and X. Wang Sensory Responses during Sleep in Primate Primary and Secondary Auditory Cortex J. Neurosci., December 31, 2008; 28(53): 14467 - 14480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Mallet, A. Pogosyan, L. F. Marton, J. P. Bolam, P. Brown, and P. J. Magill Parkinsonian Beta Oscillations in the External Globus Pallidus and Their Relationship with Subthalamic Nucleus Activity J. Neurosci., December 24, 2008; 28(52): 14245 - 14258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Kipke, W. Shain, G. Buzsaki, E. Fetz, J. M. Henderson, J. F. Hetke, and G. Schalk Advanced Neurotechnologies for Chronic Neural Interfaces: New Horizons and Clinical Opportunities J. Neurosci., November 12, 2008; 28(46): 11830 - 11838. [Full Text] [PDF]
|
|
|
|
|
|
H. Merchant, T. Naselaris, and A. P. Georgopoulos Dynamic Sculpting of Directional Tuning in the Primate Motor Cortex during Three-Dimensional Reaching J. Neurosci., September 10, 2008; 28(37): 9164 - 9172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Diester and A. Nieder Complementary Contributions of Prefrontal Neuron Classes in Abstract Numerical Categorization J. Neurosci., July 30, 2008; 28(31): 7737 - 7747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Niell and M. P. Stryker Highly Selective Receptive Fields in Mouse Visual Cortex J. Neurosci., July 23, 2008; 28(30): 7520 - 7536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dejean, C. E. Gross, B. Bioulac, and T. Boraud Dynamic Changes in the Cortex-Basal Ganglia Network After Dopamine Depletion in the Rat J Neurophysiol, July 1, 2008; 100(1): 385 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Montgomery, A. Sirota, and G. Buzsaki Theta and Gamma Coordination of Hippocampal Networks during Waking and Rapid Eye Movement Sleep J. Neurosci., June 25, 2008; 28(26): 6731 - 6741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I. Buia and P. H. Tiesinga Role of Interneuron Diversity in the Cortical Microcircuit for Attention J Neurophysiol, May 1, 2008; 99(5): 2158 - 2182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Atencio and C. E. Schreiner Spectrotemporal Processing Differences between Auditory Cortical Fast-Spiking and Regular-Spiking Neurons J. Neurosci., April 9, 2008; 28(15): 3897 - 3910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tang, D. Jackson, J. Hobbs, W. Chen, J. L. Smith, H. Patel, A. Prieto, D. Petrusca, M. I. Grivich, A. Sher, et al. A Maximum Entropy Model Applied to Spatial and Temporal Correlations from Cortical Networks In Vitro J. Neurosci., January 9, 2008; 28(2): 505 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zoccolan, M. Kouh, T. Poggio, and J. J. DiCarlo Trade-Off between Object Selectivity and Tolerance in Monkey Inferotemporal Cortex J. Neurosci., November 7, 2007; 27(45): 12292 - 12307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Cowen and B. L. McNaughton Selective Delay Activity in the Medial Prefrontal Cortex of the Rat: Contribution of Sensorimotor Information and Contingency J Neurophysiol, July 1, 2007; 98(1): 303 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Luczak, P. Bartho, S. L. Marguet, G. Buzsaki, and K. D. Harris Sequential structure of neocortical spontaneous activity in vivo PNAS, January 2, 2007; 104(1): 347 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Maurer, S. L. Cowen, S. N. Burke, C. A. Barnes, and B. L. McNaughton Phase Precession in Hippocampal Interneurons Showing Strong Functional Coupling to Individual Pyramidal Cells J. Neurosci., December 27, 2006; 26(52): 13485 - 13492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Likhtik, J. G. Pelletier, A. T. Popescu, and D. Pare Identification of Basolateral Amygdala Projection Cells and Interneurons Using Extracellular Recordings J Neurophysiol, December 1, 2006; 96(6): 3257 - 3265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Devilbiss, M. E. Page, and B. D. Waterhouse Locus Ceruleus Regulates Sensory Encoding by Neurons and Networks in Waking Animals J. Neurosci., September 27, 2006; 26(39): 9860 - 9872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Blanche, M. A. Spacek, J. F. Hetke, and N. V. Swindale Polytrodes: High-Density Silicon Electrode Arrays for Large-Scale Multiunit Recording J Neurophysiol, May 1, 2005; 93(5): 2987 - 3000. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
