JN AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 92: 1928-1936, 2004. First published May 12, 2004; doi:10.1152/jn.00273.2004
0022-3077/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A corrigendum has been published
Right arrow All Versions of this Article:
92/3/1928    most recent
00273.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Richter, T. A.
Right arrow Articles by Roper, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Richter, T. A.
Right arrow Articles by Roper, S. D.

Acid-Sensitive Two-Pore Domain Potassium (K2P) Channels in Mouse Taste Buds

Trevor A. Richter1, Gennady A. Dvoryanchikov1, Nirupa Chaudhari1,2 and Stephen D. Roper1,2

1Department of Physiology and Biophysics, and 2Neuroscience Program, University of Miami School of Medicine, Miami, Florida 33136

Submitted 19 March 2004; accepted in final form 7 May 2004

Sour (acid) taste is postulated to result from intracellular acidification that modulates one or more acid-sensitive ion channels in taste receptor cells. The identity of such channel(s) remains uncertain. Potassium channels, by regulating the excitability of taste cells, are candidates for acid transducers. Several 2-pore domain potassium leak conductance channels (K2P family) are sensitive to intracellular acidification. We examined their expression in mouse vallate and foliate taste buds using RT-PCR, and detected TWIK-1 and -2, TREK-1 and -2, and TASK-1. Of these, TWIK-1 and TASK-1 were preferentially expressed in taste cells relative to surrounding nonsensory epithelium. The related TRESK channel was not detected, whereas the acid-insensitive TASK-2 was. Using confocal imaging with pH-, Ca2+-, and voltage-sensitive dyes, we tested pharmacological agents that are diagnostic for these channels. Riluzole (500 µM), selective for TREK-1 and -2 channels, enhanced acid taste responses. In contrast, halothane (≤ ~17 mM), which acts on TREK-1 and TASK-1 channels, blocked acid taste responses. Agents diagnostic for other 2-pore domain and voltage-gated potassium channels (anandamide, 10 µM; Gd3+, 1 mM; arachidonic acid, 100 µM; quinidine, 200 µM; quinine, 100 mM; 4-AP, 10 mM; and TEA, 1 mM) did not affect acid responses. The expression of 2-pore domain channels and our pharmacological characterization suggest that a matrix of ion channels, including one or more acid-sensitive 2-pore domain K channels, could play a role in sour taste transduction. However, our results do not unambiguously identify any one channel as the acid taste transducer.

Address for reprint requests and other correspondence: S. Roper, Dept. of Physiology and Biophysics, University of Miami School of Medicine, 1600 NW 10th Ave., Miami, FL 33136 (E-mail: Roper{at}miami.edu).




This article has been cited by other articles:


Home page
 JDRHome page
Y. Ishimaru and H. Matsunami
Transient Receptor Potential (TRP) Channels and Taste Sensation
Journal of Dental Research, March 1, 2009; 88(3): 212 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
S. Kataoka, R. Yang, Y. Ishimaru, H. Matsunami, J. Sevigny, J. C. Kinnamon, and T. E. Finger
The Candidate Sour Taste Receptor, PKD2L1, Is Expressed by Type III Taste Cells in the Mouse
Chem Senses, March 1, 2008; 33(3): 243 - 254.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
P. M. Wise, J. L. Hansen, D. R. Reed, and P. A.S. Breslin
Twin Study of the Heritability of Recognition Thresholds for Sour and Salty Taste
Chem Senses, October 1, 2007; 32(8): 749 - 754.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Ishimaru, H. Inada, M. Kubota, H. Zhuang, M. Tominaga, and H. Matsunami
Transient receptor potential family members PKD1L3 and PKD2L1 form a candidate sour taste receptor
PNAS, August 15, 2006; 103(33): 12569 - 12574.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. A. DeFazio, G. Dvoryanchikov, Y. Maruyama, J. W. Kim, E. Pereira, S. D. Roper, and N. Chaudhari
Separate Populations of Receptor Cells and Presynaptic Cells in Mouse Taste Buds
J. Neurosci., April 12, 2006; 26(15): 3971 - 3980.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Maruyama, E. Pereira, R. F. Margolskee, N. Chaudhari, and S. D. Roper
Umami responses in mouse taste cells indicate more than one receptor.
J. Neurosci., February 22, 2006; 26(8): 2227 - 2234.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
V. Lyall, H. Pasley, T.-H. T. Phan, S. Mummalaneni, G. L. Heck, A. K. Vinnikova, and J. A. DeSimone
Intracellular pH Modulates Taste Receptor Cell Volume and the Phasic Part of the Chorda Tympani Response to Acids
J. Gen. Physiol., December 27, 2005; 127(1): 15 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Behav Cogn Neurosci RevHome page
A. C. Spector and S. P. Travers
The representation of taste quality in the Mammalian nervous system.
Behav Cogn Neurosci Rev, September 1, 2005; 4(3): 143 - 191.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
Y.-J. Huang, Y. Maruyama, K.-S. Lu, E. Pereira, I. Plonsky, J. E. Baur, D. Wu, and S. D. Roper
Mouse Taste Buds Use Serotonin as a Neurotransmitter
J. Neurosci., January 26, 2005; 25(4): 843 - 847.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the The American Physiological Society.