JN Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 95: 1491-1498, 2006. First published November 23, 2005; doi:10.1152/jn.00697.2005
0022-3077/06 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
95/3/1491    most recent
00697.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (28)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, R.
Right arrow Articles by Routh, V. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, R.
Right arrow Articles by Routh, V. H.

Effects of Oleic Acid on Distinct Populations of Neurons in the Hypothalamic Arcuate Nucleus Are Dependent on Extracellular Glucose Levels

R. Wang1, C. Cruciani-Guglielmacci2, S. Migrenne2, C. Magnan2, V. E. Cotero1 and V. H. Routh1

1Department of Pharmacology and Physiology, New Jersey Medical School, Newark, New Jersey; and 2Université Paris 7, Centre National de la Recherche Scientifique Unité Mixte de Recherche 7059, Paris, France

Submitted 1 July 2005; accepted in final form 17 November 2005

Pharmacological manipulation of fatty acid metabolism in the hypothalamic arcuate nucleus (ARC) alters energy balance and glucose homeostasis. Thus, we tested the hypotheses that distinctive populations of ARC neurons are oleic acid (OA) sensors that exhibit a glucose dependency, independent of whether some of these OA sensors are also glucose-sensing neurons. We used patch-clamp recordings to investigate the effects of OA on ARC neurons in brain slices from 14- to 21-day-old Sprague–Dawley (SD) rats. Additionally, we recorded spontaneous discharge rate in ARC neurons in 8-wk-old fed and fasted SD rats in vivo. Patch-clamp studies showed that in 2.5 mM glucose 12 of 94 (13%) ARC neurons were excited by 2 µM OA (OA-excited or OAE neurons), whereas six of 94 (6%) were inhibited (OA-inhibited2.5 or OAI2.5 neurons). In contrast, in 0.1 mM glucose, OA inhibited six of 20 (30%) ARC neurons (OAI0.1 neurons); none was excited. None of the OAI0.1 neurons responded to OA in 2.5 mM glucose. Thus OAI2.5 and OAI0.1 neurons are distinct. Similarly, in seven of 20 fed rats (35%) the overall response was OAE-like, whereas in three of 20 (15%) it was OAI-like. In contrast, in fasted rats only OAI-like response were observed (three of 15; 20%). There was minimal overlap between OA-sensing neurons and glucose-sensing neurons. In conclusion, OA regulated three distinct subpopulations of ARC neurons in a glucose-dependent fashion. These data suggest that an interaction between glucose and fatty acids regulates OA sensing in ARC neurons.

Address for reprint requests and other correspondence: V. H. Routh, Department of Pharmacology and Physiology, New Jersey Medical School, 185 S. Orange Ave, PO Box 1709, Newark, NJ 07101-1709 (E-mail: routhvh{at}umdnj.edu)




This article has been cited by other articles:


Home page
 DiabetesHome page
A.-L. Colombani, L. Carneiro, A. Benani, A. Galinier, T. Jaillard, T. Duparc, G. Offer, A. Lorsignol, C. Magnan, L. Casteilla, et al.
Enhanced Hypothalamic Glucose Sensing in Obesity: Alteration of Redox Signaling
Diabetes, October 1, 2009; 58(10): 2189 - 2197.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Le Foll, B. G. Irani, C. Magnan, A. A. Dunn-Meynell, and B. E. Levin
Characteristics and mechanisms of hypothalamic neuronal fatty acid sensing
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2009; 297(3): R655 - R664.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. C. Haywood, A. J. Bree, E. C. Puente, D. Daphna-Iken, and S. J. Fisher
Central but not systemic lipid infusion augments the counterregulatory response to hypoglycemia
Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E50 - E56.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Becskei, T. A. Lutz, and T. Riediger
Diet-derived nutrients mediate the inhibition of hypothalamic NPY neurons in the arcuate nucleus of mice during refeeding
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2009; 297(1): R100 - R110.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y.-H. Jo, Y. Su, R. Gutierrez-Juarez, and S. Chua Jr.
Oleic Acid Directly Regulates POMC Neuron Excitability in the Hypothalamus
J Neurophysiol, May 1, 2009; 101(5): 2305 - 2316.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. A. Murphy, X. Fioramonti, N. Jochnowitz, K. Fakira, K. Gagen, S. Contie, A. Lorsignol, L. Penicaud, W. J. Martin, and V. H. Routh
Fasting enhances the response of arcuate neuropeptide Y-glucose-inhibited neurons to decreased extracellular glucose
Am J Physiol Cell Physiol, April 1, 2009; 296(4): C746 - C756.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. Kang, N. M. Sanders, A. A. Dunn-Meynell, L. D. Gaspers, V. H. Routh, A. P. Thomas, and B. E. Levin
Prior hypoglycemia enhances glucose responsiveness in some ventromedial hypothalamic glucosensing neurons
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R784 - R792.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Aja, L. E. Landree, A. M. Kleman, S. M. Medghalchi, A. Vadlamudi, J. M. McFadden, A. Aplasca, J. Hyun, E. Plummer, K. Daniels, et al.
Pharmacological stimulation of brain carnitine palmitoyl-transferase-1 decreases food intake and body weight
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R352 - R361.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
N. Marty, M. Dallaporta, and B. Thorens
Brain Glucose Sensing, Counterregulation, and Energy Homeostasis
Physiology, August 1, 2007; 22(4): 241 - 251.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
X. Fioramonti, S. Contie, Z. Song, V. H. Routh, A. Lorsignol, and L. Penicaud
Characterization of Glucosensing Neuron Subpopulations in the Arcuate Nucleus: Integration in Neuropeptide Y and Pro-Opio Melanocortin Networks?
Diabetes, May 1, 2007; 56(5): 1219 - 1227.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
N. M. Sanders, G. J. Taborsky Jr., C. W. Wilkinson, W. Daumen, and D. P. Figlewicz
Antecedent Hindbrain Glucoprivation Does Not Impair the Counterregulatory Response to Hypoglycemia
Diabetes, January 1, 2007; 56(1): 217 - 223.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
B. E. Levin, L. Kang, N. M. Sanders, and A. A. Dunn-Meynell
Role of Neuronal Glucosensing in the Regulation of Energy Homeostasis
Diabetes, December 1, 2006; 55(Supplement_2): S122 - S130.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. Migrenne, C. Cruciani-Guglielmacci, L. Kang, R. Wang, C. Rouch, A.-L. Lefevre, A. Ktorza, V. H. Routh, B. E. Levin, and C. Magnan
Fatty Acid Signaling in the Hypothalamus and the Neural Control of Insulin Secretion
Diabetes, December 1, 2006; 55(Supplement_2): S139 - S144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. E. Levin
Orexins: neuropeptides for all seasons and functions
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R885 - R888.
[Full Text] [PDF]

Home page
 Phil Trans R Soc BHome page
B. E Levin
Metabolic imprinting: critical impact of the perinatal environment on the regulation of energy homeostasis
Phil Trans R Soc B, July 29, 2006; 361(1471): 1107 - 1121.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2006 by the The American Physiological Society.