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This Article Full Text Full Text (PDF) All Versions of this Article: 95/4/2193    most recent 01135.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Hewitt, S. A. Articles by Bains, J. S. Search for Related Content PubMed PubMed Citation Articles by Hewitt, S. A. Articles by Bains, J. S.

Brain-Derived Neurotrophic Factor Silences GABA Synapses Onto Hypothalamic Neuroendocrine Cells Through a Postsynaptic Dynamin-Mediated Mechanism

Hotchkiss Brain Institute and Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada

Submitted 27 October 2005; accepted in final form 4 January 2006

In the paraventricular nucleus of the hypothalamus (PVN), experimental stress paradigms that suppress -aminobutyric acid (GABA) inputs to parvocellular neuroendocrine cells (PNCs) also increase the expression of brain-derived neurotrophic factor (BDNF). In the adult CNS, BDNF regulates the efficacy of GABAergic transmission, but its contributions to functional changes at inhibitory synapses in the PVN have not been investigated. Analysis of quantal transmission revealed a decrease in the frequency of miniature inhibitory postsynaptic currents (mIPSCs) in response to BDNF with no accompanying changes in their amplitude. These effects were completely blocked by prior inclusion of the TrKB receptor antagonist K252a in the patch pipette. Inclusion of a dynamin inhibitory peptide in the patch pipette also blocked the effects of BDNF, consistent with an all-or-none removal of clusters of postsynaptic GABA_A receptors. Finally, to confirm a decrease in the availability of postsynaptic GABA_A receptors, we tested the effects of BDNF on focal application of the GABA_A agonist muscimol. Postsynaptic responses to muscimol were reduced after BDNF. Collectively, these data indicate that BDNF remodels functional synaptic contacts putatively by reducing the surface expression of postsynaptic GABA_A receptors.

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